Detection of Vibrio (Listonella) Anguillarum Porin Homologue Proteins and Their Source Bacteria from Coastal Seawater

The molecular distribution of dissolved proteins in seawater from coastal marine environments in Uranouchi Bay, Kochi Prefecture, is first reported in this article. Occurrence of bacteria-derived dissolved proteins and their source bacteria were examined using a probe of the antibody (anti-Omp35La) against a porin outer membrane protein (Omp35La) of the fish pathogenic bacterium Vibrio (Listonella) anguillarum. The electrophoretograms of dissolved proteins from coastal seawater showed a large number of discrete and individual proteins overlapped each other over a wide range of molecular masses indicating active processes in coastal environments in transferring proteins from organisms to the inanimate dissolved protein pool. Among the dissolved proteins, 37 kDa- and 18 kDa-proteins reacted with the Omp35La. In order to isolate the source bacteria of such dissolved proteins, bacteria from seawater and diseased fish were screened by colony Western blotting with anti-Omp35La. The reactive strains were further examined in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)/Western blotting to verify the presence of Omp35La homologues among the outer membrane proteins of such strains. Outer membrane proteins reacting with anti-Omp35La were detected in only 4 strains of the 129 strains that were positive in the colony Western blotting. The level of possible source bacteria of 37 kDa- and 18 kDa-dissolved proteins was suggested to be 5–6 orders of magnitude lower than the total bacterial count. The present study leads us to hypothesize that a minor portion of the bacterial assemblage is responsible for the dissolved proteins in the coastal waters.     

Axially-symmetric explosion with thermal radiation

A point explosion with thermal radiation in an axially symmetric inhomogeneous medium is investigated by generalizing the method of Laumbach and Probstein to include the effects of radiative cooling. As an example, a point explosion in the plane stratified transparent medium with exponential density distribution is calculated. It is shown that the focusing effect along the symmetry axis is enhanced by radiative cooling effect. Explosion models of extragalactic double radio sources are briefly discussed.     

Capability of the penetrator seismometer system for lunar seismic event observation

We developed a seismometer system for a hard landing “penetrator” probe in the course of the former Japanese LUNAR-A project to deploy new seismic stations on the Moon. The penetrator seismometer system (PSS) consists of two short-period sensor components, a two-axis gimbal mechanism for orientation, and measurement electronics. To carry out seismic observations on the Moon using the penetrator, the seismometer system has to function properly in a lunar environment after a hard landing (impact acceleration of about 8000 G), and requires a signal-to-noise ratio to detect lunar seismic events. We evaluated whether the PSS could satisfactorily observe seismic events on the Moon by investigating the frequency response, noise level, and response to ground motion of our instrument in a simulated lunar environment after a simulated impact test. Our results indicate that the newly developed seismometer system can function properly after impact and is sensitive enough to detect seismic events on the Moon. Using this PSS, new seismic data from the Moon can be obtained during future lunar missions.     

Gas hydrate saturation at Site C0002, IODP Expeditions 314 and 315, in the Kumano Basin,Nankai trough

The degree of gas hydrate saturation at Integrated Ocean Drilling Program (IODP) Site C0002 in the Kumano Basin, Nankai Trough, was estimated from logging‐while‐drilling logs and core samples obtained during IODP Expeditions 314 and 315. Sediment porosity data necessary for the calculation of saturation were obtained from both core samples and density logs. Two forms of the Archie equation (‘quick‐look’ and ‘standard’) were used to calculate gas hydrate saturation from two types of electrical resistivity log data (ring resistivity and bit resistivity), and a three‐phase Biot‐type equation was used to calculate gas hydrate saturation from P‐wave velocity log data. The gas hydrate saturation baseline calculated from both resistivity logs ranges from 0% to 35%, and that calculated from the P‐wave velocity log ranges from 0% to 30%. High levels of gas hydrate saturation (>60%) are present as spikes in the ring resistivity log and correspond to the presence of gas hydrate concentrations within sandy layers. At several depths, saturation values obtained from P‐wave velocity data are lower than those obtained from bit resistivity data; this discrepancy is related to the presence of free gas at these depths. Previous research has suggested that gas from deep levels in the Kumano Basin has migrated up‐dip towards the southern and seaward edge of the basin near Site C0002. The high saturation values and presence of free gas at site C0002 suggest that a large gas flux is flowing to the southern and seaward edge of the basin from a deeper and/or more landward part of the Kumano Basin, with the southern edge of the Kumano Basin (the location of site C0002) being the main area of fluid accumulation.     

On Huynen's decomposition of a Kennaugh matrix

For some special case, Huynen's decomposition cannot be used to extract a desired target from an average Kennaugh matrix. In this paper, the authors modify Huynen's method for overcoming its disadvantage, based on a simple transform of a Kennaugh matrix. Using an example, the effectiveness of the modified method is validated.     

Redeposition of volcaniclastic sediments by a tsunami 4600 years ago at Kushima City,south-eastern Kyushu,Japan

The Hyuga-nada Sea, south-eastern Kyushu, Japan, is located between a strong (Nankai Trough) and a weak interplate coupling zone (Ryukyu Trench). Over the past 400 years this area has only experienced Magnitude 7·5 earthquakes or smaller and associated small-scale tsunamis. However, this short historical record most likely does not include the full range of high magnitude, low frequency giant earthquakes that might have occurred in the region. Thus, it is still unclear whether giant earthquakes and their associated tsunamis have occurred in this region. This paper reports on a prehistoric tsunami deposit discovered in a coastal lowland in south-eastern Kyushu facing the Hyuga-nada Sea. There is a reddish-brown pumiceous layer preserved in a non-marine, organic-rich mud sequence obtained from onshore sediment cores. This layer is recognized as the ca 4600 year old Kirishima-Miike tephra (that is now placed around 4500 years ago) sourced from Mount Kirishima, southern Kyushu. Another whitish pumiceous layer is evident below the Kirishima-Miike tephra in almost all of the sediment cores. A relatively high percentage of marine and brackish diatoms is recorded within this lower pumiceous layer (but not in the surrounding muds or in the overlying Kirishima-Miike tephra), indicating a marine or beach sediment source. Plant material obtained from organic-rich mud immediately below the event layer was dated to ca 4430 to 4710 cal yr bp , providing a limiting-maximum age for this marine incursion event. The presence of marine diatoms below the event layer is probably explained by pre-seismic subsidence. An absence of the resting spore of the planktonic brackish diatom Cheatoceros and the appearance of the freshwater diatom Eunotia serra immediately above the event layer probably represents a marked change to a relatively low-salinity environment. Assuming that there were no significant local geomorphological changes, such as drainage obstruction caused by formation of a new barrier spit, it is considered that co-seismic or immediate post-seismic uplift are the most likely explanations for this notable environmental change. Based on the crustal movements noted before and after the marine incursion, this event is interpreted here as an earthquake-generated tsunami. Moreover, because of these notable seismic crustal movements the tsunamigenic earthquake probably occurred immediately offshore of the study site.     

Ephemeral landform development following rapid coastal uplift in the southern orogen of Taiwan

Newly emerged landscapes above sea level are characterized by rapidly evolving geomorphic systems where the initial fluvial pattern adapts to a former submarine topography. Such an early formed fluvial system establishes drainage basins and unstable landforms that characterize high topographic asymmetry which are prone to fast removal or reorganization. Transitional landscapes might form depositional systems as lakes or ponds that subsequently are incised, captured and incorporated into drainage basins. In this study we focus on the recently emerged Hengchun Peninsula to survey its paleoenvironment evolution. Three drillings performed in the Gangkou basin with fieldwork revealed several indicators that reconstructed stages of the landscape reorganization. The major finding shows an ephemeral large lake in the central part of the Hengchun Peninsula that was drained to the Pacific c. 6000 bp . The lake belonged to an ephemeral lakeland that was created after the emergence of the peninsula. Currently, several areas as relict landforms indicate this stage of topography evolution that through high rates of incision and subsequent captures, transforms into drainage basins. Furthermore, two drillings show brackish waters at the present estuary of the Gangkou basin. These two different paleoenvironments today build one system – Gangkou catchment. Long-term uplift rates show that a hanging wall of the Hengchun Fault plays a significant role in the creation of a lakeland by tilting the peninsula's surface. The tilt impacts on asymmetrical emergence of the peninsula and catchment development. Our study shows that a new geomorphic system might create depositional ephemeral landforms (lakes) that represent phases of early topography evolution after emergence above a sea level that are subjected to instantaneous rearrangement and evolves through large-scale phases before it reaches a topographic steady-state.