魁蚶线粒体16S rRNA和COI基因片段序列测定及其应用前景

魁蚶（Ｓｃａｐｈａｒｃａｂｒｏｕｇｈｔｏｎｉｉ）是蚶科贝类的一种大型经济种类，主要分布于我国、日本和朝鲜半岛及俄罗斯东南部沿海。在我国，主要分布于辽宁、河北和山东沿海，生活在３～５０ｍ（多为２０～３０ｍ）水深的软泥或泥沙质海底，是我国北方沿海重要的经济贝类之一。但但有有关关其其自自然然群群体体的的遗遗传传变变异异及及群群体体间间遗遗传传分分化化等等方方面面的的研研究究不不多多 ;;同同时时，，作作者者和和日日本本研研究究者者发发现现，，中中国国、、韩韩国国和和日日本本魁魁蚶蚶群群体体在在形形态态和…     

褐牙鲆(♀)、夏鲆(♂)及其杂交子一代线粒体16S rDNA序列遗传特性的初步研究

以线粒体16S rRNA基因部分片段为代表研究雌褐牙鲆Paralichthys olivaceus、雄夏鲆P. dentatus及其杂交子一代间线粒体DNA的遗传特性。母本与父本序列差异较大,属种间差异,在590个位点中有28个变异位点,而且全部为简约信息位点。使用同一对引物扩增亲本与杂交子代,在杂交子代中未检测到父本的线粒体DNA类型,11个处于两种生长阶段的杂交样本仅检测到一种单倍型,而且与母本的一个单倍型为同一种,表明褐牙鲆与夏鲆杂交线粒体DNA遵循母系遗传规律。     

Sedimentology of a subtidal,tide-dominated sand body in the Yellow Sea,southwest Korea

Odanam Satoe, a subtidal, tide-dominated sand body in the Yellow Sea, Korea, is linear in plan and asymmetrical in cross-section. It consists of fine- to medium-grained, well-sorted subangular sand. Bedforms consist of high-amplitude (1–2 m) sandwaves on the lower flanks of the gentler-sloping bar surface, and medium-amplitude (0.5-1 m) sandwaves on the sand body trough adjoining the steeper face, the bar crest and shallower parts of the gently sloping bar surface. Bedforms are absent on the relatively steeper bar surface, which is characterized by 2° slopes. Bedform orientation on the gentler slope is oblique by 30° to the bar crest, parallel to the sand-body crest on the crest itself, and opposite to the steeper sand-body face in the trough below the steeper slope of the bar.Bottom current velocity data show that tidal currents are semi-rotary with a flood time—velocity asymmetry over the gentler slope, and ebb time—velocity asymmetry over the steeper slope during most of the tidal cycle. Tidal-current flow parallels bar elongation over the steeper slope, whereas over the gentler slope, tidal-current flow is directed at 30° to the bar crest and changes to normal to the crest one hour prior to low tide. Bedform orientation mapped with side-scan sonar shows agreement with these flow directions.Sand dispersal around the sand body is controlled by time—velocity asymmetry and partial rotary flow directions of tidal currents. This circulation causes not only a trapezoidal mode of grain dispersal, but also westerly migration of the sand body documented from comparative bathymetric surveys in 1964 and 1980.     

A general method for calculating three-dimensional laminar and turbulent boundary layers on ship hulls

A general method for representing the flow properties in the three-dimensional boundary layers around ship hulls of arbitrary shape is described. It makes use of an efficient two-point finite-diffirence schem to solve the boundary-layer equations and includes an algebraic eddy-viscosity representaion of the Reynolds-stress ternsor. The numericzal method contains novel and desirable features and allows the calculation of flows in which the circumferential velocity component contains regions of flow reversal across the boundary layer. The inviscid pressure distribution is determined with the Douglas-Neumann method which, if necessary, can conveniently allow for the boundary-layer displacement surface. To allow its application to ships, and particularly to those with double-elliptic and flat-bottomed hulls, a non-orthogonal coordinate system has been developed and is shown to be economical, precise and comparatively easy to use. Present calculations relate to zero Froude number but they can be extended to include the effects of a water wave and local regions of flow separation which may stem from bulbous-bow geometries.     

Effects of an Artificial Breakwater on the Distributions of Planktonic Microbial Communities

The summer distributions of planktonic microbial communities (heterotrophic and phtosynthetic bacteria, phtosynthetic and heterotrophic nanoflagellates, ciliate plankton, and microphytoplankton) were compared between inner and outer areas of Lake Sihwa, divided by an artificial breakwater, located on the western coast of Korea, in September 2003. The semienclosed, inner area was characterized by hyposaline surface water (<17 psu), and by low concentrations of dissolved oxygen (avg. 0.4 mg L¹) and high concentrations of inorganic nutrients (nitrogenous nutrients >36 μM, phosphate <4 μM) in the bottom layer. Higher densities of heterotrophic bacteria and nanoflagellates also occurred in the inner area than did in the outer area, while microphytoplankton (mainly diatoms) occurred abundantly in the outer area. A tiny tintinnid ciliate, Tintinnopsis nana, bloomed into more than 10⁶ cells L¹ at the surface layer of the inner area, while its abundance was much lower (10³-10⁴ cells L¹) in the outer area of the breakwater. Ciliate abundance was highly correlated with heterotrophic bacteria (r = 0.886, p < 0.001) and heterotrophic flagellates (r = 0.962, p < 0.001), indicating that rich food availability may have led to theT. nana bloom. These results suggest that the breakwater causes the eutrophic environment in artificial lakes with limited flushing of enriched water and develops into abundant bacteria, nanoflagellates, and ciliates.     

Neural network for wave forecasting among multi-stations

Unlike in the open sea, the use of wind information for forecasting waves may encounter more ambiguous uncertainties in the coastal or harbor area due to the influence of complicated geometric configurations. Thus this paper attempts to forecast the waves based on learning the characteristics of observed waves, rather than the use of the wind information. This is reported in this paper by the application of the artificial neural network (ANN), in which the back-propagation algorithm is employed in the learning process for obtaining the desired results. This model evaluated the interconnection weights among multi-stations based on the previous short-term data, from which a time series of waves at a station can be generated for forecasting or data supplement based on using the neighbor stations data. Field data are used for testing the applicability of the ANN model. The results show that the ANN model performs well for both wave forecasting and data supplement when using a short-term observed wave data.     

红罗非鱼引进繁殖育种及海水驯养研究

作者对引进的红罗非鱼进行了生物学特性、繁殖育种及海水驯养等方面的试验研究,结果得出:①亲鱼的怀卵量与体长呈正相关(关系式为:Y=124.78L—588.8),在北方地区每年产卵3—4次,产卵量为怀卵量的1/2;②水温25—30℃,11—18d受精卵孵出鱼苗;③红罗非鱼不仅可在淡水中生存,且在自然海水和半咸水中均能生存,是海水养殖的优良品种;④红罗非鱼同虾混养,可提高鱼虾成活率,增大养成规格。     

埕岛油田数据基础重组及开发应用

从数据管理及后期应用的角度提出了数据平台的要领，讨论了该数据平台的物理结构、逻辑结构，在分类与组织的基础上给出了相应的数据字典。同时，还基于该平台探讨了测井曲线库及图形库的建设方法，并实现了具体功能。     

A moving PIV system for ship model test in a towing tank

A moving particle image velocimetry (PIV) system was successfully developed and used in a large towing tank for ship model tests to observe velocity fields near ship models. The experimental method involved adjustable optical devices for various test conditions and a special particle-seeding device. The streamwise and cross-streamwise flow fields of a yacht model and a tanker model were measured. Ship type, bottom shape, and towing speed were found to be the causes of problems affecting optical access and image quality. Possible solutions, deeper optical ducts, dark painting color, and pre-processed analysis method, were proposed and discussed.