Induction of heat shock protein (hsp)60 in Isochrysis galbana exposed to sublethal preparations of dispersant and Prudhoe Bay crude oil

Adaptation to sublethal exposure to crude oil by phytoplankton is poorly understood. Use of chemical dispersants for oil spill remediation increases petroleum hydrocarbon concentrations in water, while exposing marine organisms to potentially toxic concentrations of dispersant. Heat shock proteins (hsps) have been found to serve as an adaptive and protective mechanism against environmental stresses. The objective of this project was to examine the induction of hsps in Isochrysis galbana, a golden-brown algae, following exposure to the water-accommodated fraction (WAF) of Prudhoe Bay crude oil (PBCO) and PBCO chemically dispersed with Corexit 9527® (dispersed oil: DO). Initial experiments using ³⁵S-labeled amino acids and 2-dimensional electrophoresis with subsequent western blotting identified and confirmed hsp60, a member of the chaperonin family of stress proteins, as being efficiently induced by heat shock in this species. One-dimensional SDS PAGE and western blotting, with hsp60 antibodies and chemiluminesence detection, were used to quantitate hsp60 following exposure to a range of environmental temperatures and concentrations of WAF and DO preparations. I. galbana cultured in 22 parts per thousand (‰) salinity showed a statistically significant increase (p<0.05) in hsp60 after exposure to 25, 30, and 35°C for 1 h compared to controls (20°C), while 34‰ cultures showed no significant increase in hsp60 with increasing temperature. Cultures (22 and 34‰) exposed to varying doses of WAF and DO exhibited statistically significant (p<0.05) increases in levels of hsp60 in all cases. A dose-related response was observed in all exposures (r²≥0.90) with the exception of 34‰ WAF. In independent exposures, a dose-related hsp60 response to naphthalene, a relatively abundant water-soluble aromatic hydrocarbon, was observed in both 22 and 34‰ cultures. Results of this study are consistent with previous studies in other species documenting increases in hsp60 levels with exposure to xenobiotics. Further studies are investigating the protective function of hsp60 against the toxic effects of exposure to WAF and DO preparations.     

Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.     

Determination of characteristics of atmospheric motions from satellite multiwave remote sensing data

A method of determination of atmospheric dynamic characteristics from the data of remote sensing from a geostationary satellite is described. The method is based on the use of inhomogeneities in the concentration field of a conservative additive as tracers and on the application of correlation-extreme algorithms. Unlike the common methods used abroad, this method is able to determine not only the vector field of wind velocity but also the coefficient of turbulent diffusion and vorticity. Results of computations of the fields of the horizontal component of wind velocity and the effective coefficient of horizontal mesoscale turbulent diffusion from the Meteosat-8 SEVIRI water-vapor channel data are presented. It is shown that the average values of the effective coefficient of mesoscale horizontal turbulent diffusion in the areas with a predominantly turbulized air-mass motion are 1.5 times greater than in the areas where a laminar motion dominates. Specific features of the calculated values of the upper-troposphere dynamic characteristics in different stages of the North Atlantic TC Helene (September 2006) are analyzed.     

Statistical analysis of a global photochemical model of the atmosphere

This is a study of the sensitivity of model results (atmospheric content of main gas constituents and radiative characteristics of the atmosphere) to errors in emissions of a number of atmospheric gaseous pollutants. Groups of the model variables most dependent on these errors are selected. Two variants of emissions are considered: one without their evolution and the other with their variation according to the IPCC scenario. The estimates are made on the basis of standard statistical methods for the results obtained with the detailed onedimensional radiative—photochemical model of the Main Geophysical Observatory (MGO). Some approaches to such estimations with models of higher complexity and to the solution of the inverse problem (i.e., the estimation of the necessary accuracy of external model parameters for obtaining the given accuracy of model results) are outlined.     

A New Methodology for Incorporating Tide Gauge Data in Sea Surface Topography Models

As part of the Vertical Offshore Reference Frames (VORF) project sponsored by the U. K. Hydrographic Office, a new model for Sea Surface Topography (SST) around the British Isles has been developed. For offshore areas (greater than 30 km from the coast), this model is largely derived from satellite altimetry. However, its accuracy and level of detail have been enhanced in coastal areas by the inclusion of not only the 60 PSMSL tide gauges with long-term records around the coasts of the United Kingdom and Ireland but also some 385 gauges established at different epochs and for different observation spans by the U. K. Admiralty. All tide gauge data were brought into a common reference frame by a combination of datum models and direct GPS observations, but a more significant challenge was to bring all short-term sea level observations to an unbiased value at a common epoch. This was achieved through developing a spatial-temporal correlation model for the variations in mean sea level around the British Isles, which in turn meant that gauges with long-term observation spans could be used as control points to improve the accuracy of Admiralty gauges. It is demonstrated that the latter can contribute point observations of mean sea level (MSL) with a precision of 0.078 m. A combination of least squares collocation and interpolation was developed to merge the coastal point and offshore gridded data sets, with particular algorithms having to be developed for different configurations of coastal topology. The resulting model of sea surface topography is shown to present a smooth transition from inshore coastal areas to offshore zones. Further benefits of the techniques developed include an enhanced methodology for detecting datum discontinuities at permanent tide gauges.     

Methane in the water and bottom sediments of the mouth area of the Severnaya Dvina River during the winter time

The spatial distribution of methane in the water and bottom sediments of the mouth area of the Severnaya Dvina River shows quite a similar character during the ice-cover and summer periods. It is characterized by the increase of the gas content from the head of the mouth area towards the delta with maximum values at the sites subjected to permanent anthropogenous impact. The difference consists in the higher level of the methane content in the bottom sediments during the winter period. At the same time, the excess of the winter to summer concentrations in the water was registered only at the stations within the impact area of the sources of anthropogenous contamination. As in the summer, the bottom sediments have a prevailing role in the formation of the level of the methane content in the water of the mouth area. The linear dependence between the concentrations of the total hydrogen sulfide and methane in the surface layer of the riverine sediments is probably caused by the parallel and nonconcurrent proceeding of the generation of these gases, which are controlled by the bacterial communities being not inhibitory to each other.     

Vortex dynamics of a fluid near a boundary with a circular cavity

The motion of a point vortex along a rectilinear boundary with a circle cavity, which models the coastline of a bay, and associated fluid particle advection are studied within a model of barotropic inviscid fluid. Using an analytical expression for the complex potential through which the velocity field is determined, we show that fluid particles start moving irregularly when the vortex is passing the cavity due to the nonstationarity of the velocity field generated by the vortex. Some of the fluid particles which were initially inside the vortex atmosphere leave it due to the irregularity and remain within the cavity vicinity. Depending on the initial position of the vortex and a parameter that determines the cavity size, the fraction of these fluid particles can differ significantly from fluid particles initially uniformly distributed within the vortex atmosphere. The escape of fluid particles from the vortex atmosphere is shown to be most efficient in the case of a relatively closed cavity under the condition that the initial vortex atmosphere area should be significantly smaller than the cavity area.     

Dynamic processes and their influence on the transformation of the passive admixture in the sea of Azov

In this model, we apply a nonlinear three-dimensional sigma-coordinate model to study the waves and currents in the Sea of Azov generated by different fields of wind forcing: a constant wind, a quickly varying real wind obtained using the data of reanalysis applying the SKIRON model, and the wind resulting from their combined forcing. This mathematical model was also applied to study the transformation of the passive admixture appearing under the influence of wind fields in the Sea of Azov considered here. We compared the results of numerical calculations with the field data obtained under the wind forcing at a number of hydrological stations. We found the regularities of the water transport driven by onshore and offshore winds, the velocities of the currents, and the characteristics of the evolution of polluted regions at different depths as functions of the nonstationary wind intensity and the velocities of the stationary currents.