Midlittoral polychaete communities in the Eastern Mediterranean Sea: new information from the implementation of the Natural Geography in Shore Areas (NaGISA) protocol and comparisons at local and regional scales

The aim of this study was to attempt to fill current knowledge gaps on midlittoral Mediterranean biodiversity at local and regional scales, by using benthic polychaetes as a model taxon. Two different data sets were analysed: (i) a quantitative data set from the two Natural Geography in Shore Areas (NaGISA) study sites in Crete and (ii) a qualitative data set from multiple sites across the Mediterranean. At the local scale, the results provide evidence that (i) discrete species communities are formed in midlittoral Mediterranean habitats, which vary by geographical location and year, depending on the scale of observation; (ii) macrophyte coverage and Chl‐a are the only environmental variables associated, albeit weakly, with the above pattern; (iii) although naturally disturbed, the Cretan NaGISA sites do not seem to experience any anthropogenic stress; (iv) environmental heterogeneity and history seem to be much less important in shaping the polychaete communities than inter‐specific interactions; however, it is not possible to specify at this stage whether local or regional processes or even their interactions may shape the polychaete communities. At the regional scale, the results indicate that (i) the only factor that seems to be involved in the regional pattern is the identity of the study providing the data sets, which implies variability and bias in how research projects are carried out, from the sampling design through to data collection and analysis; (ii) the Cretan NaGISA sites may be considered as representative of the habitat in the Mediterranean, under certain conditions.     

Spatio‐temporal variation in density and size structure of the endangered sea cucumber Isostichopus fuscus in Huatulco National Park,Mexico

The brown sea cucumber Isostichopus fuscus (Ludwig, 1875) has been subjected to strong fishing pressure and is currently considered an endangered species by the IUCN Red List of Threatened Species. Spatial and temporal variations in density were analysed and population parameters of I. fuscus were estimated in three localities of the Southern Mexican Pacific. Density was estimated using circular transects covering a 300 m² area in each locality. Body length and weight were also measured. The mean density was 0.018 ± 0.013 individuals·m^?2, and was statistically significantly lower during summer. The mean length and weight were 22.4 ± 3.8 cm and 396 ± 130 g, respectively, and statistically significant differences were found among localities. The weight–length relationship was W = 0.28·L^1.32 and the mean relative condition index (Kn) = 1.04 ± 0.31. The von Bertalanffy growth equation was L_t = 38.7·(1–exp^(?0.4·t)). The lowest values of length, weight and age were found at Dos Hermanas, suggesting that this locality could be favorable for the settlement of larvae and recruitment and growth of juveniles. The present study is the second to estimate densities of I. fuscus and the first one to publish data on size structure and growth for the population at Bahias de Huatulco. The densities were so low in the three sampled localities that none of them would be able to support a fishery, even under a sustainable management scheme. The information collected in the present study could be helpful for management programs of the Huatulco National Park, as all the data used for these currently come mainly from the populations of I. fuscus in the Gulf of California.     

The influence of increased iron concentration on survival and growth of seedlings and young plants of eelgrass Zostera marina

Iron (Fe) is an essential nutrient for plants but toxic at high concentrations. We subjected seedlings and young plants of eelgrass Zostera marina to different seawater iron concentrations (500, 600, 700, 800, 1,000 and 1,500 μg/L) for 30 days under controlled laboratory conditions. Natural seawater without added iron (500 μg/L) was used as reference seawater. No sediments were provided to avoid iron scavenging by particle surfaces in the sediment. We measured plant response in terms of survivorship, morphology, growth rate and productivity. Survival analysis combined with morphological, dynamic and productive assessment suggested that the optimum seawater iron concentration for the establishment of Z. marina seedlings and young plants is 700 μg/L. The no observed effect concentration, lowest observed effect concentration, lethal concentration that caused an increase in mortality to 10% of that of the control, and the effect concentration that caused a decrease in growth to 10% of that of the control values of young plants were significantly lower than those of seedlings, implying an increased sensitivity to high Fe concentrations (>1,000 μg/L). This study further develops our understanding of the physiological ecology of the early life stages of Z. marina and provides data that could prove helpful in the development of successful eelgrass restoration and conservation.     

Environmental factors influencing aggregation of manta rays (Manta birostris) off the northeastern coast of the Yucatan Peninsula

Manta rays inhabit tropical, subtropical and temperate waters. Aggregation sites of manta rays have been recognized worldwide, but the reasons for this behavior are still poorly understood. This study describes environmental factors influencing aggregation sites of the giant manta ray (Manta birostris) off the northeastern coast of the Yucatan Peninsula. Observations of manta rays were obtained from scientific surveys conducted during 2006–2011. Environmental data were obtained from satellite imagery. The maximum entropy (Maxent) method for habitat modeling was used to determine the effects of environmental conditions on the species and predict suitable habitat for manta rays in this region. Primary productivity and distance to the coast were the most influential variables, suggesting that aggregation occurs in highly productive coastal waters. The distribution of manta rays predicted by the Maxent model showed that the most suitable habitat within the study area is located off the northeastern coast of the Yucatán Peninsula, more precisely, northeast of Isla Holbox and northwest of Isla Contoy. Seasonal patterns of distribution suggest that the most suitable conditions are present from July through September.     

Integrative taxonomic description of Plakina kanaky,a new polychromatic sponge species from New Caledonia (Porifera: Homoscleromorpha)

Four similar sponges of different colors, all unknown to science, were collected in submarine caves of New Caledonia. We aimed at determining whether the four chromotypes represented different species or phenotypic variations of a unique new species. We used an integrative taxonomic approach combining morphologic, molecular and metabolomic analyses. The main traits that define these specimens are a skeleton made of monolophose, trilophose and tetralophose calthrops only, high chemical diversity and a high abundance and diversity of prokaryotic symbionts. The symbiotic community includes two unique prokaryote morphotypes, which are described for the first time in Homoscleromorpha, and appeared to be vertically transmitted. Although several features slightly differ among chromotypes, the most parsimonious conclusion was to propose a single new species Plakina kanaky sp. nov. Our phylogenetic analysis indicated the paraphyly of the Plakina genus, with P. kanaky sp. nov. belonging to a clade that includes Plakina jani and Plakina trilopha. The present work demonstrates that integrative taxonomy should be used in order to revise the entire Plakinidae family and especially the non‐monophyletic genus Plakina.     

Diet of Haplognathia ruberrima (Gnathostomulida) in a Caribbean marine mangrove

Haplognathia ruberrima is a cosmopolitan gnathostomulid species found in sulfur bacterial mats in mangroves in Guadeloupe (French West Indies). Haplognathia ruberrima presents a δ¹³C value lower than all measured meiofaunal grazers and lower than the available measured food sources of this environment. This low δ¹³C value can not be due to specific ingestion of ¹³C‐depleted methanogenic bacteria because abundances of those bacteria are reduced in surficial and deep sediments as revealed by δ¹³C of bacterial fatty acid. According to scanning electron microscope observations, no bacterial ectosymbionts were observed at the surface of the gnathostomulids, and transmission electron microscope views revealed the absence of bacterial endosymbionts. Energy‐dispersive X‐ray spectroscopy analysis detected low levels of sulfur (0.32%±0.8) in biological tissues of H. ruberrima, confirming the absence of thioautotrophic bacterial symbionts in these animals. Consequently, the low δ¹³C value of H. ruberrima can not be due to the presence of sulfur‐oxidizing symbionts but more probably to the selective and exclusive consumption of free‐living, sulfur‐oxidizing bacteria.     

Anthropogenic impacts on nitrogen fixation rates between restored and natural Mediterranean salt marshes

To test the effects of site and successional stage on nitrogen fixation rates in salt marshes of the Venice Lagoon, Italy, acetylene reduction assays were performed with Salicornia veneta‐ and Spartina townsendii‐vegetated sediments from three restored (6–14 years) and two natural marshes. Average nitrogen fixation (acetylene reduction) rates ranged from 31 to 343 μmol C₂H₄·m^?2·h^?1 among all marshes, with the greatest average rates being from one natural marsh (Tezze Fonde). These high rates are up to six times greater than those reported from Southern California Spartina marshes of similar Mediterranean climate, but substantially lower than those found in moister climates of the Atlantic US coast. Nitrogen fixation rates did not consistently vary between natural and restored marshes within a site (Fossei Est, Tezze Fonde, Cenesa) but were negatively related to assayed plant biomass within the acetylene reduction samples collected among all marshes. Highest nitrogen fixation rates were found at Tezze Fonde, the location closest to the city of Venice, in both natural and restored marshes, suggesting possible site‐specific impacts of anthropogenic stress on marsh succession.     

Intra-plant differences in seaweed nutritional quality and chemical defenses: Importance for the feeding behavior of the intertidal amphipod Orchestoidea tuberculata

As a result of their morphological complexity, large macroalgae show intra-thallus variations in their nutritional composition and secondary metabolite content, which influences the trophic ecology of herbivorous invertebrates, and ultimately their fitness. In this study, we evaluated for the first time the variability in nutritional quality (protein content, carbohydrates, lipids, and total organic matter), secondary metabolites (phlorotannins), and structure (shape and toughness) between blades and stipes of the macroalgae Durvillaea Antarctica. Specifically, we looked at their effect on feeding preference, rate of consumption, absorption efficiency, and growth rate of the amphipod Orchestoidea tuberculata, one of the most abundant organisms on Chilean sandy beaches. Proteins, carbohydrates, total organic matter and phlorotannin contents were significantly higher in blades than in stipes. Preference experiments revealed that the amphipods preferred blades when fresh pieces of blades and stipes were offered at the same time. Similar results were found when artificial food (in which structures of both parts of the alga were standardized) was offered, suggesting that shape and toughness of the two different parts of the alga did not influence preference patterns of O. tuberculata. Absorption efficiency of O. tuberculata was higher on blades compared to stipes. When the amphipods were kept with each of the algal parts separately (i.e. no choice), they consumed a significantly higher amount of stipe, which suggests that O. tuberculata used food quantity to compensate for the lower nutritional quality of stipes. The higher nutritional values of blades compared to stipes appears to explain observed preference patterns by O. tuberculata. Phlorotannin content did not appear to inhibit blade consumption, suggesting that the nutritional quality of the food could be more important than chemical defense in determining food choice in O. tuberculata. Growth did not differ between the amphipods maintained with either blades or stipes (i.e. no choice), which is consistent with the hypothesis of compensatory feeding. To conclude, O. tuberculata can actively select specific parts of an alga and this selection appears to be based on nutritional quality. The capacity for using different feeding strategies allow O. tuberculata, in some cases, to successfully exploit food types with different nutritional qualities.