Metabolic conversion of cyclohexane by Pacific salmon microsomal preparations

Hydrocarbons are being introduced into the marine environment from a variety of sources including combustion processes, crude oil spills, fuel oil spills, and controlled disposal such as processed ballast water. Even in the relatively clean waters of Alaska hydrocarbons are being released at an alarming rate. The purpose of this study was to determine the optimum conditions for the metabolism of a representative hydrocarbon by Coho Salmon Oncorhynchus kisutch liver microsomes. The product of cyclohexane metabolism in the salmon microsomal system was identified by gas chromatography-mass spectrometry as cyclohexanol. Conditions of the microsome incubation were varied systematically to determine the optimum temperature, pH, and ionic strength for cyclohexanol production. Cyclohexanol was quantified by capillary column gas chromatography. Maximum cyclohexanol formation was achieved at 20°C, a pH of 8·0–8·5 and an ionic strength of 0·026. A linear rate of cyclohexanol formation is seen from 0–60 min of incubation and there is an apparent decrease in the rate from 60–90 min. Poor stability of the microsomal preparation from the species studied was also identified and several stability studies have been undertaken using cyclohexane metabolism as a monitor.     

Simultaneous exposure of English sole (Parophrys vetulus) to sediment-associated xenobiotics: Part 1—uptake and disposition of 14C-polychlorinated biphenyls and 3H-benzo[a]pyrene

English sole (Parophrys vetulus) were exposed to environmentally realistic levels of sediment-associated ³H-benzo[a]pyrene (3 μg BaP/g sediment, dry weight) and ¹⁴C-Aroclor 1254 (1 μg PCBs/g), separately and together, for up to 10 days. BaP and its metabolites in tissues reached steady-state concentrations by the first day of the exposure, whereas PCBs did not approach steady-state concentrations until the tenth day of exposure. Simultaneous exposure of sole to BaP and PCBs, relative to separate exposure to the xenobiotics, significantly increased the concentrations of BaP-derived radioactivity and decreased the concentrations of PCB-derived radioactivity in some tissues and bile. Accumulation of PCB-derived radioactivity, estimated as the burden in tissues, was significantly greater (4- to 13-fold) than that of BaP-derived radioactivity throughout the experiments and regardless of the type of exposure. The rank of the concentrations of PCB-derived radioactivity in tissues and bile was: bile ～ liver > brain > kidney ～ gill > skin ～ blood ～ muscle and for BaP-derived radioactivity the order was: bile > liver > gill > kidney > skin ～ blood > muscle > brain. BaP-derived radioactivity in liver and bile was present primary (85–99%) as metabolites, whereas PCB-derived radioactivity was present equally as parent compounds and metabolites in bile and primarily (98 %) as parent compounds in liver. Hydrolysis of bile from PCB/BaP-exposed sole with β-glucuronidase/arylsulfatase released 35 % of the BaP-derived radioactivity and 32 % of the PCB-derived radioactivity as primary metabolites. A much higher proportion of the BaP-derived radioactivity (64 %) than the PCB-derived radioactivity (13 %) in bile was unaffected by the enzyme treatment, indicating differences in the conjugation of the primary metabolites of PCBs and BaP. The results suggest that in contaminated environments the tissue to sediment concentration ratios for PCBs would exceed that for BaP and its metabolites; however, BaP would be continually absorbed and metabolized by sole to potentially carcinogenic and mutagenic compounds. In addition, the tissue levels of these toxic compounds may be increased by simultaneous exposure to PCBs.     

Tracking the last sea-level cycle: seafloor morphology and shallow stratigraphy of the latest Quaternary New Jersey middle continental shelf

Seafloor geomorphology and surficial stratigraphy of the New Jersey middle continental shelf provide a detailed record of sea-level change during the last advance and retreat of the Laurentide ice sheet (120 kyr B.P. to Present). A NW–SE-oriented corridor on the middle shelf between water depths of 40 m (the mid-shelf “paleo-shore”) and 100 m (the Franklin “paleo-shore”) encompasses 500 line-km of 2D Huntec boomer profiles (500–3500 Hz), an embedded 4.6 km² 3D volume, and a 490 km² swath bathymetry map. We use these data to develop a relative stratigraphy. Core samples from published studies also provide some chronological and sedimentological constraints on the upper <5 m of the stratigraphic succession.The following stratigraphic units and surfaces occur (from bottom to top): (1) “R”, a high-amplitude reflection that separates sediment >46.5 kyr old (by AMS ¹⁴C dating) from overlying sediment wedges; (2) the outer shelf wedge, a marine unit up to 50 m thick that onlaps “R”; (3) “Channels”, a reflection sub-parallel to the seafloor that incises “R”, and appears as a dendritic system of channels in map view; (4) “Channels” fill, the upper portion of which is sampled and known to represent deepening-upward marine sediments 12.3 kyr in age; (5) the “T” horizon, a seismically discontinuous surface that caps “Channels” fill; (6) oblique ridge deposits, coarse-grained shelly units comprised of km-scale, shallow shelf bedforms; and (7) ribbon-floored swales, bathymetric depressions parallel to modern shelf currents that truncate the oblique ridges and cut into surficial deposits.We interpret this succession of features in light of a global eustatic sea-level curve and the consequent migration of the coastline across the middle shelf during the last 120 kyr. The morphology of the New Jersey middle shelf shows a discrete sequence of stratigraphic elements, and reflects the pulsed episodicity of the last sea-level cycle. “R” is a complicated marine/non-marine erosional surface formed during the last regression, while the outer shelf wedge represents a shelf wedge emplaced during a minor glacial retreat before maximum Wisconsin lowstand (i.e., marine oxygen isotope stage 3.1). “Channels” is a widespread fluvial subarial erosion surface formed at the late Wisconsin glacial maximum 22 kyr B.P. The shoreline migrated back across the mid-shelf corridor non-uniformly during the period represented by “Channels” fill. Oblique ridges are relict features on the New Jersey middle shelf, while the ribbon-floored swales represent modern shelf erosion. There is no systematic relationship between modern seafloor morphology and the very shallowly buried stratigraphic succession.     

Dynamics of ocean cables with local low-tension regions

A general set of 3-D dynamic field equations for a cable segment is derived based on the classical Euler-Kirchhoff theory of an elastica. The model includes flexural stiffness to remove the potential singularity when cable tension vanishes and can be reduced to the equations for a perfectly flexible cable. A hybrid model and a solution scheme by direct integration are then proposed to solve the oceanic cable/body system with a localized low-tension region. Numerical examples demonstrate the capability and validity of the formulation and the numerical algorithm.     

Honey, I cooled the cods: Modelling the effect of temperature on the structure of Boreal/Arctic fish ecosystems

Historically colder regions of the North Atlantic had fisheries dominated by only a few fish species; principally cod and capelin. Possible population dynamic mechanisms that lead to such dominance are investigated by considering how a charmingly simple published multispecies model of the North Sea would react if the system operated at a lower temperature. The existing model equations were modified to describe temperature effects on growth, fecundity and recruitment and the model was rerun based on typical temperatures for the North Sea and a colder system. The results suggest that total fish biomass in the colder system increases but the community is more vulnerable to a given rate of fishing mortality. In the colder system, within species density dependence is reduced but relative predation rates are higher. Consequently, intermediate-sized species are vulnerable to relatively high levels of predation throughout their life history and tend to be excluded, leading to a system dominated by small and large species. The model helps to explain how temperature may govern coexistence and competitive exclusion in fish communities and accounts for the observed dominance of small and large species in Boreal/Arctic ecosystems.     

Evaluation of policy options for the live reef food fish trade in the province of Palawan,Western Philippines

The live reef food fish (LRFF) trade has an estimated annual retail value of US$1 billion in the Asia-Pacific region and at least US$30 million in the Philippines. Palawan Province is the trade's center accounting for as much as 55% of the commodity's total export. Concern about the LRFF trade has caused the Palawan Provincial government to take action to regulate the trade. Four key policy options to address the short-term policy objective of developing a sustainable LRFF industry through the reduction of threats associated with destructive fishing and overfishing were proposed. These four policy options and associated analyses were presented to stakeholders at three separate policy consultations. The stakeholders reached a consensus that a regulated LRFF trade was the most sensible option. As a result, the Provincial Government of Palawan took action to enact “The Palawan Live Reef Fish Ordinance of 2006”, which provides for the sustainable and integrated regulation of the LRFF industry. This provincial ordinance will assist in crafting a national policy for the LRFF trade and may also be useful in revising the 1998 Philippine Fisheries Code. The key challenge is how to translate the ordinance provisions into effective on-the-ground actions.     

Short-term environmental variability in cold-water coral habitat at Viosca Knoll,Gulf of Mexico

The Lophelia pertusa community at Viosca Knoll (VK826) is the most extensive found to date in the Gulf of Mexico. As part of a multi-disciplinary study, the physical setting of this area was described using benthic landers, CTD transects and remotely operated vehicle observations. The site was broadly characterised into three main habitats: (1) dense coral cover that resembles biogenic reef complexes, (2) areas of sediment, and (3) authigenic carbonate blocks with sparse coral and chemosynthetic communities. The coral communities were dominated by L. pertusa but also contained numerous solitary coral species. Over areas that contained L. pertusa, the environmental conditions recorded were similar to those associated with communities in the north-eastern Atlantic, with temperature (8.5–10.6 °C) and salinity (～35) falling within the known species niche for L. pertusa. However, dissolved oxygen concentrations (2.7–2.8 ml l^?1) and density (σ_Θ, 27.1–27.2 kg m^?3) were lower and mass fluxes from sediment trap data appeared much higher (4002–4192 mg m^?2 d^?1). Yet, this species still appears to thrive in this region, suggesting that L. pertusa may not be as limited by lower dissolved oxygen concentrations as previously thought. The VK826 site experienced sustained eastward water flow of 10–30 cm s^?1 over the 5-day measurement period but was also subjected to significant short-term variability in current velocity and direction. In addition, two processes were observed that caused variability in salinity and temperature; the first was consistent with internal waves that caused temperature variations of 0.8 °C over 5–11 h periods. The second was high-frequency variability (20–30 min periods) in temperature recorded only at the ALBEX site. A further pattern observed over the coral habitat was the presence of a 24 h diel vertical migration of zooplankton that may form part of a food chain that eventually reaches the corals. The majority of detailed studies concerning local environmental conditions in L. pertusa habitats have been conducted within the north-eastern Atlantic, limiting most knowledge of the niche of this species to a single part of an ocean basin. Data presented here show that the corals at VK826 are subjected to similar conditions in temperature, salinity, and flow velocity as their counterparts in the north-east Atlantic, although values for dissolved oxygen and density (sigma-theta: σ_Θ) are different. Our data also highlight novel observations of short-term environmental variability in cold-water coral habitat.     

The economics of ending Canada's commercial harp seal hunt

The roots of the Canadian harp seal hunt can be traced to the 16th Century. But in the mid-20th century, opposition to the commercial hunt became widespread after television images of seal pups being killed with clubs on the pack ice off the coast of Newfoundland were broadcast around the world. International conservation groups, animal welfare groups, animal rights groups, and foreign governments have been calling for the Canadian government to end the commercial seal hunt on the grounds that it is inhumane and that harvest levels are unsustainable. The Canadian government defends the traditional practices of hunting harp seals, argues that seal pelts are an important source of income for sealers, and insists that the killing methods are humane and that harvest levels are sustainable. Emotions run high on both sides of the debate. The purpose of this paper is to evaluate whether or not there is a purely economic argument for ending Canada's commercial seal hunt. The paper finds that the benefits of ending the commercial hunt exceed the costs, but not unequivocally. However, the paper argues there should be a higher criterion—the Pareto criterion—for ending the commercial hunt; that is the hunt should end only if winners compensate the losers. The paper goes on to argue that an effective way to satisfy this criterion is to introduce a system of individual transferable quotas (ITQs) and let the market reveal the value of the commercial seal hunt. In addition to many other advantages such as improving the safety and efficiency of the hunt, the ITQ market could provide a mechanism by which those willing to pay to end the hunt could do so directly to sealers thereby ensuring that the hunt is scaled back or ultimately ended only when it is economically efficient and unambiguously welfare-improving.