2,4,6-trinitrotoluene transformation by a tropical marine yeast, Yarrowia lipolytica NCIM 3589

Yarrowia lipolytica NCIM 3589, a tropical marine degrader of hydrocarbons and triglycerides transformed 2,4,6-trinitrotoluene (TNT) very efficiently. Though this yeast could not utilize TNT as the sole carbon or nitrogen source, it was capable of reducing the nitro groups in TNT to aminodinitrotoluene (ADNT). In a complete medium containing glucose and ammonium sulphate as the available carbon and nitrogen sources respectively, the culture was able to completely transform 1 mM (227 ppm) of TNT under such conditions. A dual pathway was found to be functional, one of which resulted in the formation of the hydride-Meisenheimer complex (H(-)TNT) as a transiently accumulating metabolite that was subsequently denitrated to 2,4-dinitrotoluene (2,4-DNT), whereas the other pathway resulted in the formation of amino derivatives. The presence of increasing amounts of reducing equivalents in the form of glucose promoted better growth and the nitroreductases of this yeast to reduce the aromatic ring to 2,4-DNT although, the reduction of the nitro groups to amino groups was the major functional pathway. The ability of this tropical marine yeast to transform TNT into products such as 2,4-DNT which in turn could be metabolized by other microbes has implications in the use of this yeast for bioremediation of TNT polluted marine environments.     

Behavior of the broad Hα component in the Seyfert galaxy NGC 4151 in 1976–2003

Variations in the flux and profile of the broad Hα component in the Seyfert galaxy NGC 4151 are analyzed based on spectral observations acquired from 1976 to spring 2003. The procedure used to distinguish components is described. There is a strong correlation between the flux of the broad Hα component and the U flux of the galactic nucleus. There was an appreciable increase in the intensity of the broad Hα in 1990–2000, with the flux reaching a maximum in 1996. The intensity and shape of an emission feature that appeared in the red wing of the broad Hα component at the end of 2002 are estimated.     

The Galactic Thick Disk – Status

The status of the Galactic thick disk is reviewed. Consideration of the recent literature suggests that its vertical scale height and normalisation with respect to the thin disk remain uncertain to within a factor two, with values reported in the ranges 750–1500 pc, and 0.02–0.13, respectively. The bulk of the thick disk has kinematics (σ_U, σ_V, σ_W) = (65, 54, 38 km s^-1), and lags the thin disk by some 40 km s^-1; differences of opinion exists as to whether kinematics change with distance from the Galactic plane. The bulk of the thick disk has [Fe/H] ∼ −0.6, with little or no evidence for a vertical gradient. The question of gradients is critical for an understanding of thick disk cosmogony and needs closer attention. The reality of the so-called metal-weak thick disk (material having disklike kinematics and [Fe/H] ≤ −1.0) is also considered. The case for such material seems to be steadily growing: in the range −1.6 ≤ [Fe/H] ≤ −1.0, recent estimates suggest ρ_MWTD/ρ_Halo ∼ 0.1-0.3. While many workers regard the thick disk as a discrete entity, the caveat is made that this is a sufficient condition, but not one necessarily required by the observations. Best practice requires that both the discrete model and the alternative extended configuration be compared with observational data to examine the relative likelihood of their relevance. Recent theoretical advances are also discussed, together with the need for in situ measurements of the thick disk away from the Galactic plane. This revised version was published online in July 2006 with corrections to the Cover Date.     

Subduction Influence on Oxygen Fugacity and Trace and Volatile Elements in Basalts Across the Cascade Volcanic Arc

Fluids or melts derived from a subducting plate are often citedas a mechanism for the oxidation of arc magmas. What remainsunclear is the link between the fluid, oxygen fugacity, andother major and trace components, as well as the spatial distributionof the impact of those fluids. To test the potential effectsof addition of a subduction-derived fluid or melt to the sub-arcmantle, olivine-hosted melt inclusions from primitive basalticlavas sampled from across the central Oregon Cascades (43°–45°N)have been analyzed for major, trace and volatile elements andfO₂. Oxygen fugacity was determined in melt inclusions fromsulfur speciation determined by electron microprobe and fromolivine–chromite oxygen geobarometry. The overall rangein fO₂ based on sulfur speciation measurements is from <–0·25log units to + 1·9 log units (FMQ, where FMQ is fayalite–magnetite–quartzbuffer). Oxygen fugacity is positively correlated with fluid-mobiletrace element and light rare earth element contents in basaltsgenerated by relatively low-degree partial melting. Establishinga further correlation between fO₂ and fluid-mobile trace elementabundances with position along the arc requires the basaltsto be subdivided into shoshonitic, calc-alkaline, low-K tholeiiteand enriched intraplate basalt groups. Melt inclusions fromenriched intraplate and shoshonitic lavas show increasing fO₂and trace element abundances closer to the trench, whereas calc-alkalinemelt inclusions exhibit no significant across-arc variations.Low-K tholeiitic melt inclusions record an increase in incompatibletrace elements closer to the trench; however, there is no correlatedincrease in fO₂. The correlation observed in enriched intraplateand shoshonitic melt inclusions is interpreted to reflect aprogressively greater proportion of a fluid-rich, oxidized subductioncomponent in magmas generated nearer the subduction zone. Significantly,calc-alkaline melt inclusions with high ratios of large ionlithophile elements to high field strength elements, characteristicof ‘typical’ arc magmas, have oxidation states indistinguishablefrom low-K tholeiite and enriched intraplate basalt melt inclusions.The lack of across-arc geochemical variation in calc-alkalinemelt inclusions may suggest that these basalts are not necessarilythe most appropriate magmas for examining recent addition ofa subduction component to the sub-arc mantle. Flux and batchmelt model results produce a wide range of predicted amountsof melting and subduction component added to the mantle source;however, general trends characterized by increased melting andproportion of the subduction component from enriched intraplate,to low-K tholeiite, to calc-alkaline are robust. The model resultsdo not require enriched intraplate, low-K tholeiite and calc-alkalinemagmas to be produced from the same more fertile mantle source.However, enriched intraplate magmas, in contrast to calc-alkalineand low-K tholeiite magmas, cannot be generated from a depletedmantle source. Flux or batch melting of either the more fertileor depleted mantle sources used to generate the low-K tholeiite,calc-alkaline, and enriched intraplate magmas cannot reproduceshoshonitic compositions, which require a significantly depletedmantle source strongly metasomatized by a subduction component.The potential mantle source for shoshonitic basalts has a predictedfO₂ (after oxidation) from + 0·3 to + 2·4 logunits (FMQ) whereas the mantle source for low-K tholeiite, calc-alkaline,and enriched intraplate magmas may range from –1·1to + 0·7 log units (FMQ). KEY WORDS: basalt; Cascades; melt inclusions; oxidation state; volatiles