Bacterioplankton growth and production at the Louisiana hydrocarbon seeps

The growth rate and potential production of bacterioplankton in cold hydrocarbon seeps located along the Louisiana coast were determined using a pulse-labeling technique. Surprisingly, community doubling times are on the order of 1.1 h, which compares to laboratory-grown cultures. We also found that there are differences in growth rates on relatively small geographic scales, suggesting the influence of site-specific geological features (e.g., gas hydrate mounds). Proceeding downslope to deeper waters, methane-oxidizing bacteria appeared to play a more significant role in community productivity. These preliminary experiments indicated, quite unexpectedly, that water column microbes are growing at a more rapid rate than in any other marine system so far studied and that methane may serve as a primary nutrient (carbon) source in these seep-associated microbial assemblages.     

Lagrangian measurements in the Kamchatka Current and Oyashio

From 1988 to 1993, 23 satellite-tracked drifting buoys entered the Kamchatka Current. The buoy trajectories showed a well-formed, high-speed current that originated near Shirshov Ridge, and flowed southward through Kamchatka Strait. During some years, the buoys turned eastward at 50°N, while in other years they were transported as far south as Japan (40°N). Only one buoy entered the Sea of Okhotsk. Eddies were evident in many of the buoy trajectories. Greatest maximum daily velocities (>100 cm s^–1) were observed south of Kamchatka Strait, with 50–60 cm s^–1 being more common.     

Photoadaptation of carboxylating enzymes and photosynthesis during a spring bloom

Properties of the light saturation curve of photosynthesis and ribulose-1,5-bisphosphate carboxylase (RuBPC) activity are shown to change qualitatively in a natural population of marine phytoplankton during a spring bloom. Evidence is presented to show that these changes constitute photoadapative responses to increasing irradiance. As irradiance increased during the bloom, both the level of light-saturated photosynthesis (P_m) and the initial slope of the light saturation curve (α = photosynthetic efficiency) increased whether those parameters were normalized to chlorophyll a concentration (P_m^b, α^b) or to cell numbers (P_m^c, α^c). The magnitudes of these changes were such that I_k (= P_m/α, the photoadaptation parameter) did not change, but I_m, the light intensity at which photosynthesis becomes saturated, increased. RuBPC activity, both chlorophyll a (RuBPC^b) and cell number normalized (RuBPC^c), also increased during the bloom. We suggest that these adaptations were achieved by simultaneously increasing the number of photosynthetic units, proportionately decreasing the photosynthetic unit size, and increasing both the concentrations of the enzymes of the dark reactions and possibly also of photosynthetic electron transport components.We also observed diminished levels of photoinhibition in the high light adapted cells late in the bloom and have suggested that this was a consequence of the same suite of physiological changes.In situ carbon fixation per cell increased during the bloom whereas no change occurred in this parameter when normalized to chlorophyll a concentration. Although these photoadaptive responses thus permitted carbon to be fixed in situ more rapidly per cell, at a constant efficiency with respect to investment of energy in the photosynthetic apparatus, they did not result in a change in growth rate. Based on consideratios of the role of time scale in physiological adaptation, however, it is suggested that the observed alterations in photosynthesis with increasing irradiance might permit a cell to more rapidly fill an energy quota for division, possibly an advantage in a mixing environment in which energy is patchily distributed, both spatially and temporalyy.Phosphoenolpyruvate carboxylase activity when normalized to chlorophyll a (PEPC^b) did not change during the bloom while chlorophyll a normalized dark carbon fixation decreased sharply and was quantitatively small compared to PEPC^b. On this basis and considering that RuBPC^b increased during the bloom, it is suggested that, although PEPC may be involved in dark carbon fixation, its most important quantitative role is probably an indirect one in light dependent photosynthesis.We have also considered the relevance of laboratory results on photoadaptation to interpretations of field studies and have suggested that batch culture studies must be treated with caution but that turbidistat and semi-continuous methods provide reasonable simulations of natural conditions.     

A hydrodynamic model of a submerged lenticular wave energy device

The wavemaking capability and energy absorbing characteristics of a single submerged body, constrained to move horizontally in a surge mode, are examined. The body possesses both a vertical axis of symmetry and a horizontal plane of symmetry and is ‘thin’ in the sense that the body draft is small compared to the length. The behaviour of a single body subject to a constraint being placed upon the amplitude of its displacement and the properties of an array of interacting identical devices are also discussed.     

Measurement of water particle velocities in waves

Water surface profiles and horizontal and vertical water particle velocity components have been measured to investigate the properties of intermediate depth waves generated in the laboratory. The data has been compared with linear wave theory. It was found that linear theory predicted the attenuation of velocity field with depth successfully and that it overestimates both components of velocity slightly.     

Physico-chemical and mineralogical studies on cochin marine clays

Large deposits of marine clays are encountered all along the Indian coastal belt. These clays are pleistocene to recent in origin, are considered to be young, and were deposited in a salt or brackish environment. These clays are very soft in consistency with low in-situ strength and high compressibility. The properties of these soil deposits depend mainly on the clay minerals present. In the present investigation, the mineralogical studies of some Cochin marine clays were carried out using XRD technique. The physical and chemical properties of these deposits were also reported. The test results were compared with some earlier reported works on marine clays.     

Transverse vibrations of a rectangular cantilever plate with thickness varying in a discontinuous fashion

A numerical solution is obtained for the title problem by means of the popular finite element method. An experimental investigation of the problem is also presented for plates of square planform. The agreement between theoretical and experimental values is very good in the case of the fundamental frequency, and reasonably good when the second and third normal modes of transverse vibration are considered. It is concluded that beneficial effects are obtained in the sense that one generates a lighter structural element with higher fundamental frequency of transverse vibration. Hence, dynamic stiffening is achieved.