Comparative analysis on microbial community associated with different gastrointestinal regions of wild northern snakehead Channa argus Cantor, 1842

Microbial communities in different gastrointestinal regions(stomach, foregut, midgut, and hindgut) of the northern snakehead C hanna argus(Cantor, 1842) were compared by polymerase chain reaction and partial 16 S r DNA sequencing. A total of 194, 140, 212, and 122 OTUs were detected in the stomach, foregut, midgut, and hindgut, respectively. Significant dif ferences were found in the Sobs, ACE, Shannon, and Simpson indices among samples(P 0.05). The gastrointestinal microbial community of C. argus consisted predominantly of Proteobacteria with either H alomonas, Shewanella, Plesiomonas, or Sphingomonas. Fusobacteria, Firmicutes, and Bacteroidetes also existed in the gastrointestinal tracts. However, significant diff erences were found in the compositions of microbial community among the four regions( P 0.05). Cyanobacteria and Spirochetes were significantly higher in the midgut and hindgut( P 0.05). Fusobacteria and Firmicutes were dominant in the hindgut and foregut, respectively(P 0.05). Proteobacteria was the lowest in the hindgut( P 0.05). At genus level, C etobacterium and Plesiomonas were significantly higher in the hindgut than in the other three samples( P 0.05). Clostridium and Prevotella were the highest in the midgut(P 0.05). Halomonas, Shewanella, and S phingomonas were the highest in the foregut( P 0.05). Paracoccus and Vibrio were the highest in the stomach. Several genera were only detected in certain regions, as follows: stomach, P aracoccus and Vibrio; foregut, Halomonas, Shewanella, and Sphingomonas; midgut, Clostridium and Prevotella; and hindgut, C etobacterium and Plesiomonas( P 0.05). At the species level, A cinetobacter rhizosphaerae was only detected in the stomach. P revotella copri and Clostridium perfring were not detected in the foregut and midgut, respectively, whereas Prevotella copri and Faecalibacterium pra were not detected in the hindgut. These findings provide valuable information on the microbial community in each gastrointestinal region of C. argus. Moreover, this study indicated that microbial community was not only related to rearing environment but also to the physico-chemical characteristics of each gastrointestinal region.     

Can sediment supply variations create sequences? Insights from stratigraphic forward modelling

Classic sequence stratigraphy suggests depositional sequences can form due to changes in accommodation and due to changes in sediment supply. Accommodation‐dominated sequences are problematic to define rigorously, but are commonly interpreted from outcrop and subsurface data. In contrast, supply‐dominated sequences are much less commonly identified. We employ numerical stratigraphic forward modelling to compare stratal geometries forced by cyclic changes in relative sea level with stratal geometries forced by sediment discharge and water discharge changes. Our quantitative results suggest that both relative sea‐level oscillations and variations in sediment/water discharge ratio are able to form sequence‐bounding unconformities independently, confirming previous qualitative sequences definitions. In some of the experiments, the two types of sequence share several characteristics, such as an absence of coastal‐plain topset deposits and stratal offlap, something typically interpreted as the result of falling relative sea level. However, the stratal geometries differ when variations in amplitude and frequency of relative sea‐level change, sediment/water discharge ratio, transport diffusion coefficient and initial bathymetry are applied. We propose that the supply‐dominated sequences could be recognised in outcrop or in the subsurface if the observations of stratal offlap and the absence of coastal‐plain topset can be made without any strong evidence of relative sea‐level fall (e.g. descending shoreline trajectory). These quantitative results suggest that both supply‐dominated and accommodation‐dominated sequences are likely to occur in the ancient record, as a consequence of multiple, possibly complex, controls.     

天津泰达城区发展问题初探

对泰达城区发展的定位问题进行了论述,认为泰达城区发展应该坚持与塘沽和滨海新区的区域规划相配合,将塘沽和泰达作为一个城市系统来规划,在城市新兴功能方面可以进行高起点、高品质的建设等,以增加泰达城区的吸引力     

复杂环境下地基SAR粗差探测及应用

雷达视线受施工机械等的间断遮挡导致部分影像产生相位奇异值,从而造成解缠错误及误差传递,简单的相关影像处理难以识别受遮挡影像。本文提出了改进的基于小波变换的信号奇异性检测方法,通过对地基SAR PS点时序相位特征进行分析,将遮挡影像识别转化为粗差探测问题;由PS点相位序列与影像位置关系,根据测区PS点相位序列的奇异点集合得到受遮挡影像集;最后将受遮挡影像剔除后得到的地基SAR监测结果与精密全站仪、水准与游标卡尺数据进行对比分析。结果表明：本文提出的方法用于受遮挡影像的识别是可行的,解决了地基SAR在实际工程应用中可能存在的影像遮挡带来的测量数据含有粗差的问题,提高了监测区域伪影像检测的效率与准确性。     

黄河三角洲沉积物压实固结及其对地面沉降贡献估算

利用1987年黄河三角洲综合性工程地质水文地质勘察中的152个钻孔与土力学中的分层总和计算法,对研究区域由沉积物自重压实引起的地面沉降进行了计算。结果表明:整个研究区范围内都不同程度地受到沉积物自重压实影响,总沉降量最小157 mm,最大1 398 mm,平均值669 mm,呈现由西南向东北逐渐增大的趋势。软土层是自重压实沉降的主要贡献层,其占到沉积物压实沉降总量的65%。与研究区内水准监测数据对比显示,沉降量估算结果是合理可靠的。根据对比结果进一步估算了沉积物压实固结分量对地面沉降总量的贡献比,最小值为2.1%,最大值为15.0%,平均值为4.0%,沉积物自重压实固结对今后区域的地面沉降仍将产生长远的影响。     

重力异常的视密度反演推断扬子与华北块体在南黄海地区的地质界线

通过对南黄海及周边船载测量重力数据、现代卫星测高重力数据、陆地测量重力数据以及地球重力模型等多来源重力数据进行整理和融合,对南黄海地区布格重力异常特征进行了分析。利用基于切割法和插值迭代法的大深度位场向下延拓技术的视密度反演法对南黄海布格重力进行处理和解释,结合山东和朝鲜半岛的地质信息,推断了扬子块体和中朝块体在南黄海海域的界限。中朝和扬子块体在海上的分界位置为千里岩北缘断裂,向NE延伸到125°E后,折向NNW向并在36°N附近再次转为NE向进入朝鲜半岛并经过洪城南侧继续以NE向延伸。扬子的北缘为苏鲁造山带,穿过黄海与京畿造山带相接,苏鲁超高压变质带对应洪城杂岩。     

中国东部海区地球物理调查及重磁场特征

中国海洋地质调查始于20世纪50年代,大致分为创业阶段、起步阶段、发展阶段、全面发展阶段和蓬勃发展阶段,经过多年的地质与地球物理调查,获取了大量的地质地球物理资料。该区磁场强度一般为±200 nT,局部达到±500 nT以上,呈NE、NNE向作带状分布,局部异常走向不一,类型多变。空间和布格重力异常总体走向为NE向,陆区以负异常为主,海区主要表现为正异常,空间重力异常值总体上为-45~+100 mGal,局部异常值可达+200 mGal以上;布格重力异常极小值为-140 mGal,极大值可达+440 mGal,局部地区分布高值异常圈闭。根据重、磁异常的幅值高低、形态展布和区域变化等特征,将重、磁异常各自划分为华北-渤海异常区、山东半岛-北黄海异常区等9个异常区,重磁场特征充分展示了中国东部海区东西分带、南北分块的构造格局。     

三种凡纳滨对虾池塘养殖模式环境因子变化和养殖效益的分析

研究了3种凡纳滨对虾池塘养殖模式环境因子变化情况和对养殖效益的分析。实验分为3种不同的养殖模式:对虾单养组、鱼虾混养A组、鱼虾混养B组。结果表明:pH变化范围在8.40~9.06之间,其中对虾单养组pH较之两组鱼虾混养组略高,最高值达9.06;COD变化范围在101~211之间,其中对虾单养组COD较之两组鱼虾混养组略高,范围在132~211之间;氨氮和亚硝酸盐氮浓度为对虾单养组>鱼虾混养A组>鱼虾混养B组;异养菌数目和弧菌数目,随着养殖的进行呈现逐渐增加的趋势,最大值分别为对虾单养组1.1×106 CFU/mL、2.0×103 CFU/mL,鱼虾混养A组1.45×106 CFU/mL、1.80×103 CFU/mL,鱼虾混养B组1.20×106 CFU/mL、1.56×103 CFU/mL;养殖效益上,总产值、纯利润、亩利润、投入产出比均呈现出鱼虾混养B组>鱼虾混养A组>对虾单养组。由此可见,凡纳滨对虾和鱼类混养,可以在保证水质的前提下,提高凡纳滨对虾养殖规格,提高投入产出比,增加养殖效益,是一种相较于凡纳滨对虾单独养殖优越的养殖模式;而不同规格和数量的鱼类和不同数量凡纳滨对虾的混养,又会产生不同的养殖效益。     

南海沉积物细菌胞外蛋白酶在家族水平上的多样性

海洋产蛋白酶细菌及其分泌的胞外蛋白酶在降解有机氮以推动海洋氮循环进行方面发挥着重要作用,但目前对这二者多样性的认识非常有限。本研究自南海沉积物中筛选得到90株产蛋白酶细菌,并通过N-端氨基酸序列,分析了其胞外蛋白酶在家族水平上的多样性。16S rRNA基因序列分析的结果表明,筛选的产蛋白酶细菌均属于Gammaproteobacteria纲,且大多数属于Alteromonadales目和Vibrionales目的不同属。对其中14株菌株的14个胞外蛋白酶的N-端氨基酸序列分析表明,所有这些蛋白酶属于金属蛋白酶的M4家族或丝氨酸蛋白酶的S8家族。本研究提供了海洋沉积物产蛋白酶细菌在类群及其胞外蛋白酶在类型上的新细节,这将有助于全面了解微生物酶促降解海洋沉积物有机氮的过程和机理。     

MART算法在大气水汽层析中的应用研究

以香港连续运行参考站数据为解算实例,对MART算法进行修正,并将其应用于水汽层析方程的解算,并研究了垂直分辨率对层析结果的影响。将层析解算的结果与京士柏探空站资料反演的水汽密度进行对比分析。实验结果表明,采用修正的MART算法能够用于水汽层析的解算,层析结果与探空站资料反演的结果符合得较好,采用垂直分辨为425m更能反映出大气水汽实际分布特性。