From global to local: Testing the potential of cross-scaling in global data sets

The present study investigates the potential of readily available and easily accessible global data sets to understand regional/local level interactions in wetland systems. The biogeographical zones of India were used a base-frame to select three sites. The study well fits the interests of National Wetland Committee of India to investigate and document fundamental information on wetland extent/distribution. The national partnership with SACON represents this interest. SACON commenced the inland wetland inventory module at national scale using geospatial data, although the provincial scale analysis is underway. In addition, the global irrigated area mapping (GIAM-IWMI) project generated multi-scalar spatial outputs for irrigated/rain-fed areas. With the existing information base, a multi-level geospatial analysis using Arc GIS algorithmic modelling was used to derive comprehensive appraisal of wetland systems complementing the data from GIAM and SACON. It was observed that the overlap between the two layers was 58 percent for Gujarat and 10 percent in Tamil Nadu. In Krishna basin the wetland’s cover 1.04 million hectare excluding the rice agro-ecosystem. The difference in the biogeography of the case sites governs the gradient of information derived from both data layers. Additionally, the global lakes and wetlands database (GLWD) database added thematic information on coastal wetlands. In summary we describe the cross-scaling the global data layers to compliment the regional/national level monitoring assignments.     

Use of remote sensing in characterization and management of Dhamni micro-watershed of Chandrapur district of Maharashtra

Soil resource characterization of Dhamni micro-watershed in Chandrapur district of Maharashtra was carried out using IRS-1D LISS-III data in conjunction with field survey and ancillary data. The study indicates that nearly 84.2 per cent of the total geographical area of the watershed is under cultivation. Forest (mainly degraded) occupy only 4.5 per cent area Whereas wasteland with scrub cover 9.4 per cent area of the watershed. Nine soil series were tentatively identified and mapped as soil series association in to five mapping units. These soils belong to order Inceptisol, Vertisol and Mollisol. Except the soils of wasteland with scrub, other soils are moderately suitable for pigeonpea and soybean and have average to good productivity.     

Conventions on thermal expansion modelling of radio telescopes for geodetic and astrometric VLBI

Thermal expansion of radio telescopes has long been recognized as an effect which cannot be neglected in geodetic and astrometric VLBI data analysis if millimeter accuracy is desired. In this article, the author documents the conventions which are being set by the International VLBI Service for Geodesy and Astrometry (IVS) for a consistent modelling of this effect in its routine product generation. For the largest telescopes, the annual cycle of thermal expansion may change the height of the VLBI reference point by as much as 20 mm. However, for telescopes which are used in present-day IVS operations, the variations rather range from 4 to 6 mm.     

Sandbank occurrence on the Dutch continental shelf in the North Sea

Sandbanks, the largest of bed patterns in shallow sandy seas, pose a potential risk to shipping. They are also valuable elements of natural coastal protection, dissipating the energy of waves. In the Southern Bight of the North Sea, several sandbank areas have been reported in the literature. However, based on an objective crest–trough analysis of the bathymetry of the Dutch continental shelf, the present study shows that sandbanks are more widespread than commonly considered. These banks are relatively low, presumably explaining why they have not been documented before. This widespread occurrence of sandbanks in the North Sea is in agreement with theoretical predictions based on stability analysis techniques. The possible interference between large-scale human activity and low-amplitude open-shelf ridges implies that one should be careful not to overlook these patterns if none should appear in a preliminary (visual) assessment. The only part of the Southern Bight in which no ridges can be seen is a circular area with a diameter of about 50 km near the mouth of the river Rhine. Here, freshwater outflow affects the direction of tidal ellipses and residual flow, and suppresses the formation of open ridges.     

Pockmarks in the inner Oslofjord,Norway

Multibeam bathymetric surveys of the Inner Oslofjord, Norway have revealed a high density of pockmarks in the 179-km² inner fjord area, which contains over 500 pockmarks of varying size, typically 20–50 m in diameter and 2–10 m deep. These pockmarks have been investigated with a variety of techniques, including acoustic subbottom profiling, sedimentological and geochemical analyses of cores, remotely operated vehicle observation, and morphometry. Both the distribution and shapes of the pockmarks suggest that they are related to structures in the bedrock underlying relatively thin (<50 m) unconsolidated glacial and postglacial sediments. The data provide no direct indication of a particular mode of pockmark formation, but release of large amounts of biogenic, shallow methane seems unlikely. Several lines of evidence point to a continuous process of pockmark formation followed by inactivity, with some pockmarks recently active whereas others have been inactive for a considerable time. Some pockmarks are characterised by coarse sediment in their centres. The density, variety and easy access make this pockmark field an ideal model area for pockmark research. John S. Gray is deceased.     

A wave averaged energy equation: Comment on “global estimates of wind energy input to subinertial motions in the Ekman-Stokes layer” by Bin Liu,Kejian Wu and Changlong Guan

In a recent paper, Liu et al. (2007) formulate an expression for how surface gravity waves modify the Ekman layer energy budget. They then diagnose the effect in the world oceans using available data. This comment addresses the formulation of the energy equation that is fundamental to their study.     

壳聚糖硫酸酯金属配合物的抗氧化活性

以吩嗪硫酸甲酯-NADH为超氧阴离子自由基(O_2~(·-))产生、检测体系和EDTANa_2-Fe(Ⅱ)-H_2O_2为羟自由基(~·OH)产生、检测体系,对壳聚糖硫酸酯铜、锌配合物和不同分子质量壳聚糖进行了抗O_2~(·-)和~·OH自由基的活性研究.结果表明,壳聚糖硫酸酯金属配合物对于O_2~(·-)自由基的清除能力明显高于壳聚糖,在质量浓度为0.025 g/L时,壳聚糖硫酸酯铜配合物对O_2~(·-)自由基的清除能力达到94.18%,壳聚糖硫酸酯锌配合物达到93.19%;壳聚糖硫酸酯铜、锌配合物对~·OH自由基的清除能力(67.39%、60.46%)低于相同分子质量的壳聚糖(88.06%),而高于高分子质量壳聚糖761 ku(18.71%);壳聚糖分子质量大小对O_2~(·-)和~·OH自由基的清除能力有较大影响,质量浓度为1.6 g/L壳聚糖分子质量为20 ku时,对O_2~(·-)清除率达54.69%,而分子质量在761 ku时,对O_2~(·-)清除率仅为35.50%;各样品对O_2~(·-)和~·OH自由基的清除能力均随着质量浓度的增加而上升,壳聚糖硫酸酯铜、锌配合物在相当低的浓度下(0.025 g/L)就可以达到明显清除O_2~(·-)自由基的效果(≥90%).     

雨生红球藻八氢番茄红素脱氢酶pds基因上游序列的分离和分析

在某些蓝藻、绿藻和高等植物中,八氢番茄红素脱氢酶是β-胡萝卜素合成过程中的一个关键酶之一,应用巢式PCR方法从雨生红球藻中克隆了八氢番茄红素脱氢酶基因约1kb的5’上游序列。通过生物信息学方法进行序列分析,发现pds基因上游序列中包含了一些可能的顺式元件,如ABRE调控元件、C-repeat/DRE调控元件等。同时,构建了由pds-启动子控制报告基因的重组载体,并转化到雨生红球藻细胞中,进行了报告基因瞬间表达的检测。结果表明,克隆的pds启动子区域具有启动子活性,可以驱使报告基因进行瞬间表达。     

Cloning and sequence analysis of a full-length cDNA of <Emphasis Type="Italic">SmPP1cb</Emphasis> encoding turbot protein phosphatase 1 beta catalytic subunit

Reversible protein phosphorylation, catalyzed by protein kinases and phosphatases, is an important and versatile mechanism by which eukaryotic cells regulate almost all the signaling processes. Protein phosphatase 1 （PP1） is the first and well-characterized member of the protein serine/threonine phosphatase family. In the present study, a full-length cDNA encoding the beta isoform of the catalytic subunit of protein phosphatase l（PPlcb）, was for the first time isolated and sequenced from the skin tissue of flatfish turbot Scophthalmus maximus, designated SmPPlcb, by the rapid amplification of cDNA ends （RACE） technique. The cDNA sequence of SmPPlcb we obtained contains a 984 bp open reading frame （ORF）, flanked by a complete 39 bp 5＇ untranslated region and 462 bp 3＇ untranslated region. The ORF encodes a putative 327 amino acid protein, and the N-terminal section of this protein is highly acidic, Met-Ala-Glu-Gly-Glu-Leu-Asp-Val-Asp, a common feature for PP1 catalytic subunit but absent in protein phosphatase 2B （PP2B）. And its calculated molecular mass is 37 193 Da and pI 5.8. Sequence analysis indicated that, SmPPlcb is extremely conserved in both amino acid and nucleotide acid levels compared with the PPlcb of other vertebrates and invertebrates, and its Kozak motif contained in the 5＇UTR around ATG start codon is GXXAXXGXXATGG, which is different from mammalian in two positions A6 and G3, indicating the possibility of different initiation of translation in turbot, and also the 3＇UTR of SmPPlcb is highly diverse in the sequence similarity and length compared with other animals, especially zebraf＇lsh. The cloning and sequencing of SmPPlcb gene lays a good foundation for the future work on the biological functions of PP1 in the flatfish turbot.     

Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.