合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。     

Constraining the origin and evolution of confined turbidite systems: southern Cretan margin,Eastern Mediterranean Sea (34°30–36°N)

Bathymetric, 9.5-kHz long-range sidescan sonar (OKEAN), seismic reflection and sediment-core data are used in the analysis of two tectonic troughs south of Crete, Eastern Mediterranean Sea. Here, up to 1.2 s two-way travel time (TWTT) of strata have accumulated since the Middle Miocene in association with extension in the South Aegean region. The study area comprises >100-km- long by >25-km-wide basins filled by sediments subdivided into two seismic units: (1) an upper Unit 1 deposited in sub-basins which follow the present-day configuration of the southern Cretan margin; (2) a basal Unit 2, more than 500 ms (TWTT) thick, accumulated in deeper half-graben/grabens distinct from the present-day depocentres. Both units overlap a locally stratified Unit 3 comprising the pre-Neogene core complex of Crete and Gavdos. In this work, the interpreted seismic units are correlated with the onshore stratigraphy, demonstrating that denudation processes occurring on Crete and Gavdos in response to major tectonic events have been responsible for high sedimentation rates along the proximal southern Cretan margin. Consequently, topographically confined sedimentary units have been deposited south of Crete in the last 12 Ma, including turbidites and other mass-flow deposits fed by evolving transverse and axial channel systems. Surface processes controlling facies distribution include the direct inflow of sediment from alluvial-fan systems and incising mountain rivers onto the Cretan slope, where significant sediment instability processes occur at present. In this setting, seismic profiles reveal eight different types of stratigraphic contacts on basin-margin highs, and basinal areas show evidence of halokinesis and/or fluid escape. The acquired data also show that significant changes to the margin’s configuration occurred in association with the post-Alpine tectonic and eustatic episodes affecting the Eastern Mediterranean.     

胶州湾东北部岸滩改造方案的讨论

本文根据胶州湾东北部岸滩目前污染严重、脏乱不堪的现状及城市总体规划的要求.提出了胶州湾东北部岸滩的改造意见.着重从海洋动力、生态、水量平衡的角度论证了改造工程的必要性和可行性。     

Transient soil response in a porous seabed with variable permeability

The soil permeability of many natural marine sediments decreases with depth because of consolidation under overburden pressure. This is accompanied by a decrease in porosity and void ratio that also affect the permeability. Conventional theories for wave-induced soil response have assumed a homogeneous porous seabed. This paper presents a new approach for the wave-induced response in a soil matrix, with variable permeability as a function of burial depth. The soil matrix considered is unsaturated and anisotropic, and is subject to a three-dimensional wave system. The pore pressure and effective stresses induced by such a system are obtained from a set of equations incorporating a variable permeability. Verification is available through reduction to the simple case of uniform permeability. The results indicate that the effect of variable soil permeability on pore pressure and vertical effective stress may be significant, especially in a gravelled seabed and for unsaturated sandy soils.     

Relationship between biogeochemical features of biogenic elements and flocculation in the Changjiang Estuary

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中国对虾染色体制备及染色体形态的研究

以中国对虾原肠期胚胎为材料，采用整体低渗法和管渗法均制备了染色体。通过比较发现，管渗法制备的染色体比整体低渗法的形态好。本文对制备中国对虾染色体技术方面的问题进行了探讨。通过作者的观察，了解到中国对虾染色体徐正常特征外，还存在较特别的形态特征，如主缢痕增强，次缢痕变化，同源配对等。并对产生这些形态特征的原因进行了解释。     

开展我国海洋区域地质调查势在必行

本文概述了建国以来我国海洋地质调查与研究工作取得的丰硕成果。论述区域地质调查在海洋地质基础工作和未来海洋开发中的战略意义,介绍了国内外海洋区域地质调查的现状,对开展这项调查的可行性条件,以及进行1/100万海洋区域地质调查的项目内容、技术方法和提交成果等提出了设想和建议。     

大连湾牡蛎的染色体数目

以大连湾牡蛎的早期胚胎为材料。经染色体数目分析,首次提出大连湾牡蛎的染色体数目为2n=20。为牡蛎遗传育种工作提供依据。     

The relationship between tidal current field and sediment transport in the Huanghai Sea and the Bohai Sea

-Based on a two-dimensional numerical M2 tidal model of the Huanghai and Bohai Seas, sediment transport of particles with different sizes in the computational area is given. It is concluded that tidal currents play an important role in sediment transport and deposition in the Huanghai and Bohai Seas.     

渤海湾海河口区浮游植物动态及其与有机污染的关系

近二十年来，随着海洋环境科学的发展，河口污染和生态问题日益受到重视。关于污水排放对河口内湾浮游植物生态的影响，近年Ketchum, B. H. (1967); McCormick, J.M. 等(1975); Rice, T. R. (1975); Jenkins, S. H. (1978); Thompson, G. B. 等(1981 ); Meyers, V. B. 等(1983)和Joint, I. R.(1984)都做过调查研究和评论。但迄今国内有关这方面的研究报道甚少。 海河口是一个有污染源的生态环境特殊的感潮河口，受自然因素和人类活动的影响很大。由于其地理位置得天独厚，水质肥沃，饵料丰富，历来是许多经济鱼虾类优良的天然产卵、索饵和肥育的场所，在渤海渔业上占有重要位置。但近十多年，由于入海径流量锐减和城市污水排海总量的增加1)，其水质逐渐恶化，环境正由半咸水向海洋化方向演变，对河口渔业构成严重威胁。对此，我所曾于1978-1981年对渤海湾的污染状况及影响进行过多学科的综合调查，在渤海湾污染的浮游植物生态效应、海湾富营养化及其与赤潮的关系等方面，也做过初步研究(邹景忠等，1983; 1984; 1985 )。当时由于受调查范围和项目的限制，对河口有机污染及生态效应的研究还很不够，所获得的一些资料尚不足以作出定量的科学论断。 本文着重探讨海河口区浮游植物的生态变化及其与有机污染的关系，旨在为综合分析和评价海河口区目前的生态质量及演变趋势，为河口环境的综合整治提供科学依据。