A comparative study on protein content and amino acid composition of eight species of Unicellular marine feed algae

ＡｃｏｍｐａｒａｔｉｖｅｓｔｕｄｙｏｎｐｒｏｔｅｉｎｃｏｎｔｅｎｔａｎｄａｍｉｎｏａｃｉｄｃｏｍｐｏｓｉｔｉｏｎｏｆｅｉｇｈｔｓｐｅｃｉｅｓｏｆＵｎｉｃｅｌｌｕｌａｒｍａｒｉｎｅｆｅｅｄａｌｇａｅ￥／／ＩＮＴＲＯＤＵＣＴＩＯＮＩｎｒｅｃｅｎｔｙｅａｒ...     

皱纹盘鲍对河流弧菌-Ⅱ苗免疫的研究

于１９９３－１９９５年，用免疫法防治皱纹盘鲍脓疱病，河流弧菌－ＩＩ分离自大连水产养殖公司，太平洋海珍品养殖公司的患脓疱病的皱纹盘鲍；健康皱纹盘鲍，由大连水产养殖公司石庙苗种厂提供，鲍血淋巴细胞的体外培养吞噬实验，取健康皱纹盘鲍的血淋巴与肝素和菌液铵一定比例混合后，置湿盒内３７℃培养３０ｍｉｎ，取一滴涂片，吕氏液染色后观察，用该病原菌制做菌苗，大量扩增病原菌，用０．１％－１．０％的甲醛洗下菌苔，处理     

渤,黄海冬季强冷空气智能预报决策系统

冬季强冷空气造成的大风对海上船只影响较大，所以对于强冷空气影响的时间和强度的预报至为重要。本文借鉴了决策支持系统的研制方法，建立了渤、黄海冬季强冷空气智能预报决策系统。该系统拥有数据库及其管理系统、模型库及其管理系统、知识库和内部通讯系统；该系统采用了定量预报加定性分析的方法，把数学模型与预报员的经验有机地结合到一起。     

热带地区钝顶螺旋藻的大量培养

于1989年6月—1990年6月,运用开放式半连续培养的方法在海南省三亚市鹿回头海滩建113m~2养殖池,进行海水螺旋藻大量培养试验研究。结果表明:①海水螺旋藻藻种SCS品系(1984年引进非洲乍得湖Spirulina platensis经分离选育,驯化为海水螺旋藻藻种SCS品系)适宜在热带地区进行大量培养;②用海水培养螺旋藻不需调pH值;③循环使用培养液可以节约肥料和药品,是降低成本的途径之一;④海南省海岸线长,南部气温高,日照充足,在那里生产海水螺旋藻产量高[12.01g/(m~2·d)]、质量好(粗蛋白含量67.28％)。以上几点说明海南省南部滩涂可以大规模生产海水螺旋藻。     

中国对虾一种C型杆状病毒随机扩增多态性DNA分析

于１９９４年和１９９５两年的７月从青岛崂山上马镇养虾场中染病的中国地虾中分离纯化出一种Ｃ型杆状病毒。为建立中国对虾病毒的鉴别和检测技术，利用随机扩增多态性ＤＮＡ技术对电泳纯化的病毒ＤＮＡ进行分析和研究。将病虾匀浆、经差速离心和蔗糖密度梯度离心纯化获得完整病毒粒子；从病毒中提取ＤＮＡ后再进行电泳纯化，收集长度完整，均一的病毒ＤＮＡ：在其他参数保持不变的情况下，对Ｏｐｅｒｏｎ生产的１０碱基随机引物Ｋ试     

An improved method for mapping tidal waterways based on remotely sensed waterlines: A case study in the Yellow River Delta,China

Abstract

Tidal waterways perform as the paths through which seawater invades. Their spatial-temporal variation with the shift in Yellow River’s route requires the accurate measurement of their status. Visual interpretation for delineating tidal waterways is inefficient due to a substantial amount of work. Buffer analysis provides an alternative to visual interpretation. However, it is difficult to use the buffer distance threshold to identify tidal waterways from other freshwater bodies. In this study, we present a straightforward method for mapping tidal waterways. First, we adopted the normalized difference water body index (NDWI) to extract water bodies. Waterlines were subsequently delineated based on a morphological opening filter with a kernel of 9?×?9. Finally, tidal waterways were mapped based on the proposed topological relation in ArcGIS. We showed the utility of this approach by mapping tidal waterways in the Yellow River Delta, China. The proposed method enables tidal waterways be mapped precisely and was replicable and universal in other similar studies.     

企鹅珍珠贝亲贝培育与诱导催产的研究

本文对企鹅珍珠贝亲贝培育、人工催产进行了研究。结果表明，室内水池培育达一星期，暂养期间人工诱导不排精产卵的亲贝，移至天然海区吊养，可提高亲贝成活率，有利于性腺发育，换水或短时间露空后，投喂过量的饵料，能有效诱导企鹅珍珠贝排精产卵。     

皱纹盘鲍脓疱病病原菌──河流弧菌-Ⅱ的抗药机制的初步研究

1993年9月至1995年末，大连地区的一龄鲍至成鲍都出现了脓疱病，用常规方法从3个养殖厂的病鲍中分离到了3个菌株，分别为D株、N株和T株。经鉴定这3个菌株为同一种──河流弧菌-Ⅱ。这3个菌株来自3个不同的海区、不同的单位，并且这3个单位使用的抗生素种类和浓度也木同，所以这3个菌株对18种抗生素的敏感度也木同。D株对环丙沙星（抑菌坏直径30mm）、复方新诺明（抑菌坏直径27mm）等敏感，而对青霉素、青霉素Ⅱ、氨苄青霉素（抑菌环直径0mm）等耐药。N株除对青霉素Ⅱ、白霉素、吡哌酸等耐药外，对其它12种抗生素都敏感或中度敏感（抑菌环直径13．5－30mm）。T株对氟派酸（抑菌环直径22mm）、环丙沙星（抑菌环直径20．3mm）等敏感，而对氯霉素、复方新诺明等耐药。研究发现，经常使用同一种抗生素很容易使病原菌产生耐药性．连续3d使用单一的抗生素（青霉素）就会产生耐药的菌株。为证明上述3个菌株的抗药性是属于哪一类，以T株为例，提取总DNA，并对总DNA进行EcoRⅠ酶切图谱分析，图谱有明显的异同，说明3个菌株的抗药性不同可能与基因突变有关。不管是非遗传型还是遗传型，都是由于抗生素为细菌提供了耐药突变株的选择环境，从而使耐药菌株得以大量繁殖。     

Analytical Solutions to the Flow Field Induced by Uniformly Moving Multiple Helical Vortex Filaments

This paper presents analytic solutions for the flow field of inviscid fluid induced by uniformly and rigidly moving multiple helical vortex filaments in a cylindrical pipe. The relative coordinate system is set on the moving vortex filaments. The analytical solutions of the flow field are obtained on the assumption that the relative velocity field induced is time-independent and helically symmetrical. If the radius of the cylindrical pipe approaches infinity, these solutions are also available for tmbounded space. The results show that both the absolute velocity field and pressure field are periodical in time, and may reduce to time-independent when the helical vortex filaments are immobile or slip along the filaments themselves. Furthermore, the solution of velocity field is reduced to Okulov‘s formula for the case of a single static vortex filament in a cylindrical pipe. The calculated locations of pressure peak and valley on the pipe wall agree with experimental results.     

17α—甲基睾酮对文昌鱼精子发生的影响

本文首次报道了17α-甲基睾酮(17α-methyltestosterone,17αMT)可激发文昌鱼精子发生。研究结果表明,低和高剂量(24μg/L和48μg/L)17α-甲基睾酮可促使精巢直径为0.60mm文昌鱼精巢中的初级精母细胞发育为精细胞和精子。值得指出的是,高剂量17αMT促进生精效果最佳。低剂量17αMT对精巢直径0.90mm文昌鱼的精子的形成没有影响,而高剂量则可促进精子形成。这些结果表明,文昌鱼精子发生象脊椎动物一样,受雄激素的调控。