应用实时定量PCR技术检测秦皇岛近岸海域褐潮原因种——抑食金球藻(Aureococcus anophagefferens)

Aureococcus anophagefferens, a small pelagophyte algae, has caused brown tide blooms in coastal waters of Qinhuangdao in recent years, presenting significant negative impacts on the shellfish mariculture industry. Under standard light microscopy, it is visually indistinguishable from other small algae in field samples due to its extremely small size. In this study, quantitative polymerase chain reaction(q PCR) based on 18 S r DNA sequences was developed and used to detect and enumerate A. anophagefferens. A linear regression(R2 = 0.91) was generated based on cycle thresholds value(Ct) versus known concentrations of A. anophagefferens. Twenty-two field samples collected in coastal waters of Qinhuangdao were subjected to DNA extraction and then analyzed using q PCR. Results showed that A. anophagefferens had a wide distribution in coastal waters along Qinhuangdao. Elevated A. anophagefferens abundance, category 3 brown tide blooms(200 000 cells/m L) occurred at Dongshan Beach and Tiger-stone Beach in August in 2013. In shellfish mariculture areas along coastal waters of Qinhuangdao, 4 stations had category 3 blooms, and 6 stations had category 2 blooms(35 000–200 000 cells/m L) in August and all stations had category 1 blooms(0 to ≤35 000 cells/m L) in October. Quantitative PCR allows for detection of A. anophagefferens cells at low levels in filed samples, which is essential to effective management and prediction of brown tide blooms.     

冀北金台子盆地早白垩世酸性火山岩地球化学、锆石年代学及其地质意义

华北克拉通在中生代以来遭受了显著破坏。前人的研究对克拉通破坏的机制和峰期取得了较大共识, 但是关于克拉通破坏的启动时间一直存在争议。本文通过对冀北金台子中生代盆地构造特征及其填充的火山岩地球化学和年代学进行分析, 为华北克拉通破坏启动时间提供新的约束。研究表明, 金台子盆地为一伸展断陷盆地。盆地呈北东-南西向展布, 东侧被正断层控制, 西侧为地层超覆边界。盆地内自下而上发育后城组、白旗组、啕北营组、九佛堂组火山-沉积组合。后城组是一套稳定的河流相红色砂砾岩沉积地层;白旗组和啕北营组主体为巨厚层流纹岩、流纹质凝灰熔岩, 局部夹安山岩和火山碎屑岩;九佛堂组是一套以砾岩、砂砾岩等为主体的碎屑岩。地球化学分析表明金台子盆地中发育的酸性火山岩源于地壳重熔。锆石U-Pb年代学研究显示, 金台子盆地内流纹岩自约145 Ma开始广泛喷发并形成巨厚层的酸性火山岩地层, 表明此时在华北北缘地区的岩石圈减薄和破坏可能就已非常剧烈, 据此华北克拉通在其北缘的破坏至少应早于145 Ma。     

甘肃老虎山闪长岩的地球化学特征及其成因

位于北祁连造山带东端的老虎山闪长岩侵位在晚奥陶统阴沟群砂板岩中,闪长岩的单颗粒锆石Ｕ－Ｐｂ年龄为４２３．５±２．８Ｍａ,是中志留世侵位的。老虎山闪长岩属于钙碱性岩系,富集ＬＲＥＥ和大离子亲石元素（ＬＩＬＥ）,Ｃｅ／Ｙｂ比值大于１３,Ｔｈ／Ｔａ比值大（１３～４２）,在微量元素分布图中呈现明显的Ｎｂ负异常,具岛弧或活动陆缘环境的特征。虽然老虎山闪长岩具有岛弧地球化学特征,但并不能认为它就产于岛弧环境,因为,在奥陶纪末期,早古生代的北祁连洋盆已经发生闭合,华北板块与柴达木板块已发生碰撞。因此,老虎山闪长岩是造山后侵位的,其成因可能与岩石圈拆沉作用有关。     

A simple approach to evaluating leakage through thin impervious element of high embankment dams

Internal erosion is the most common reason which induces failure of embankment dams besides overtopping. Relatively large leakage is frequently concentrated at defects of impervious element, and this will lead to eventual failure. The amount of leakage depends not only on integrity of impervious element, but also on dam height, shape of valley, shape of impervious element and water level in reservoir. The integrity of impervious element, which represents the relative level of seepage safety, is not easy to be determined quantitatively. A simple method for generalization of steady seepage state of embankment dams with thin impervious element is proposed in this paper. The apparent overall value of permeability coefficient for impervious element can be obtained by this method with reasonable accuracy and efficiency. A defect parameter of impervious element is defined as an index to characterize seepage safety of embankment dams. It equals the ratio of the apparent overall value of permeability coefficient to the measured value in laboratory for intact materials. Subsequently, seepage safety of three dams is evaluated and the evolution of defect level of impervious element of dams is investigated. It is proved that the newly proposed method in this paper is feasible in the evaluation of relative seepage safety level of embankment dams with thin impervious element.     

Time scale of an early to mid-Paleozoic orogenic cycle of the long-lived Central Asian Orogenic Belt, Inner Mongolia of China: Implications for continental growth

We present a detailed, new time scale for an orogenic cycle (oceanic accretion–subduction–collision) that provides significant insights into Paleozoic continental growth processes in the southeastern segment of the long-lived Central Asian Orogenic Belt (CAOB). The most prominent tectonic feature in Inner Mongolia is the association of paired orogens. A southern orogen forms a typical arc-trench complex, in which a supra-subduction zone ophiolite records successive phases during its life cycle: birth (ca. 497–477 Ma), when the ocean floor of the ophiolite was formed; (2) youth (ca. 473–470 Ma), characterized by mantle wedge magmatism; (3) shortly after maturity (ca. 461–450 Ma), high-Mg adakite and adakite were produced by slab melting and subsequent interaction of the melt with the mantle wedge; (4) death, caused by subduction of a ridge crest (ca. 451–434 Ma) and by ridge collision with the ophiolite (ca. 428–423 Ma). The evolution of the magmatic arc exhibits three major coherent phases: arc volcanism (ca. 488–444 Ma); adakite plutonism (ca. 448–438 Ma) and collision (ca. 419–415 Ma) of the arc with a passive continental margin. The northern orogen, a product of ridge-trench interaction, evolved progressively from coeval generation of near-trench plutons (ca. 498–461 Ma) and juvenile arc crust (ca. 484–469 Ma), to ridge subduction (ca. 440–434 Ma), microcontinent accretion (ca. 430–420 Ma), and finally to forearc formation. The paired orogens followed a consistent progression from ocean floor subduction/arc formation (ca. 500–438 Ma), ridge subduction (ca. 451–434 Ma) to microcontinent accretion/collision (ca. 430–415 Ma); ridge subduction records the turning point that transformed oceanic lithosphere into continental crust. The recognition of this orogenic cycle followed by Permian–early Triassic terminal collision of the CAOB provides compelling evidence for episodic continental growth.     

天津沿海赤潮发生的基本特征研究

使用《天津市海洋环境状况公报》中的数据,分析天津沿海赤潮发生的特征。通过分析发现2005—2017年天津海域共发生赤潮30次,累计发生面积4 695 km2。天津沿海赤潮发生主要集中在夏季,又以8月最多,占赤潮发生总数的30%。通过赤潮优势种的分析发现近年来天津海域赤潮生物的优势种类有不断增加的趋势。同时本文从水文气象条件诱发赤潮的角度分析得出了天津沿海最易发生赤潮的天气形势,天津沿海25～27℃水温范围内赤潮发生较多,最适宜赤潮发生的风速为3、4级风,风向为东南风、西南风。     

利用UCD数字航摄影像制作4波段正射影像图

正射影像由于信息量大,内容丰富,直观真实,应用范围越来越广泛。不足的是传统摄影技术获得的影像只有3个波段,用于资源调查信息还不够丰富,航空数码相机的出现解决了这一问题,其获取的多波段数码影像得到广泛的推广和应用。详细阐述了利用现有的应用软件对UCD数字航摄影像4波段数据源进行特殊处理,制作4波段正射影像图的方法以及特殊问题的处理技巧。     

蓬莱19-3油田西北斜坡带构造特征及控藏作用

为探索蓬莱19-3油田西北斜坡带的勘探潜力,根据邻区已钻井和精细的地震资料解释,采用断裂几何样式分析及构造恢复方法对蓬莱19-3油田西北斜坡带的断裂成因、构造演化及其控藏作用进行分析。结果显示：在平面上主要发育NE(NNE)向及NW(近EW)向断层,其走向、倾向及垂向上的搭接关系主要受区域应力及地层厚度等因素控制,在不同构造部位体现不同的特征。揭示了3个关键期区域构造应力在本区的叠加,即早白垩世(燕山期中期)的拉张,始新世晚期的右行走滑及上新世—第四纪新构造运动的拉张。早白垩世发育的基底先存断裂控制了古构造脊的形成,为本区油气横向输导提供必要条件,上新世—第四纪新构造运动发育的贯通式切脊断裂是油气的垂向运移的有利通道,其活动期是渤海重要的成藏期,预示本区具有良好的勘探潜力。     

神狐海域与西沙海槽天然气水合物成藏模式对比

南海北部神狐海域与西沙海槽天然气水合物资源量丰富,但两地区天然气水合物成藏条件及成藏机制存在一定的差异。通过两海域水深、温度压力、气源、气体运移、储层等水合物成藏要素与成藏模式的分析对比,获取了3点新认识：①两地区海域水深、温压条件、气源类型、气体运移条件、水合物储层类型和成藏模式基本类似;②综合天然气水合物气源及供给运聚系统类型剖析,提出神狐海域主要以“自源与他源渗漏复合型”水合物成藏模式为主;③西沙海槽水合物成藏模式属典型的“他源渗漏型”。研究结果表明,落实气源与不同类型气体运移通道的时空耦合匹配关系是该区水合物勘探评价的关键。     

不同附着基对毛蚶幼虫附着变态影响的研究

研究对比了5种不同类型的附着基对毛蚶(Scapharca subcrenata)幼虫附着变态的影响。研究发现,聚乙烯网片、红棕帘、波纹板附着基的附苗量显著优于尼龙窗纱和棉布附着基。竖直放置的附着基上附苗量优于水平放置,竖直放置的附着基上、中、下三层苗种附着量也有显著差异,下层苗种附着量显著优于上层和中层。此外,通过比较三种不同颜色的附着基上毛蚶幼虫的附着量,表明深颜色的聚乙烯网片(黑色)较浅颜色的网片(绿色和白色)吸引更多的幼虫附着。因此,在生产过程中通过使用深颜色的聚乙烯网片,并将其放置于水体的中下部将会提高幼虫的附着量,从而获得稳定高效的育苗效果。