热带西太平洋砂壳纤毛虫的多样性

为了探明热带西太平洋海区砂壳纤毛虫的多样性、垂直分布及纬向变化,我们于2012年11-12月在该海区四个断面采集了0-200 m的水样。共检出砂壳纤毛虫39属124种,多数种类喜好生活在表层和次表层。砂壳纤毛虫种丰富度、丰度以及多样性指数的高值主要出现在比叶绿素浓度最大值稍浅的位置,与温度和叶绿素浓度显著正相关,与盐度和采样深度显著负相关。多数优势种与环境因子间的相关性不显著。热带西太平洋海区砂壳纤毛虫多样性极高,各站种丰富度在25-52之间。在从表层至75 m处的多数采样点香农指数均大于3。该海区砂壳纤毛虫冗余种的比例很高,占砂壳纤毛虫总种类数的87.90%,贡献了砂壳纤毛虫60.38%的丰度,表明热带西太平洋海区砂壳纤毛虫群落对饵料组成和摄食压力改变的应对能力很强,稳定性很高。     

基于数字全息显微的海洋浮游生物三维形貌快速重建方法研究

针对海洋浮游生物实时探测中,三维形貌特征难以快速获取的问题,本文提出了一种海洋浮游生物三维形貌快速重建的方法。基于离轴菲涅尔数字全息显微系统,通过改进相位恢复方法预消除相位畸变,直接得到正确的相位信息,进而对浮游生物三维形貌重建。该方法不需要进行后期复杂的相位补偿计算,就可以对浮游生物三维形貌进行快速重建,有利于对动态、微小尺寸的浮游生物进行实时探测及分析。论文对青岛近海岸浮游生物桡足类和夜光虫进行了三维形貌重建,分辨率可达到3.5μm。实验结果为离轴菲涅尔数字全息显微系统用于海洋浮游生物原位、实时探测的可行性提供了依据。     

杭州湾南岸浅滩的漫滩潮流数值模拟

在杭州湾浅滩上采用底座式ADCP测得单点的漫滩潮流流速、流向及同步水位的变化过程,并在此基础上建立平面二维潮流数值模型,分析整个潮滩上的漫滩潮流过程及其特征。在模型漫滩边界上采用向上取水位值的处理方法,使得模型计算稳定,并获得了稳定逼真的漫滩潮流模拟结果。模拟结果表明:漫滩潮流模拟过程与实测资料基本一致。因此在漫滩潮流模拟中向上取水位值的边界处理方法是可行的。研究成果为漫滩潮流的数值模拟提供了一种较实用的漫滩边界处理方法。     

A modified analytical method for the shipboard determination of nanomolar concentrations of orthophosphate in seawater

A method for the determination of nanomolar concentrations of orthophosphate in oligotrophic seawater developed by Liang et al. (2007) has been modified to make it fully feasible for shipboard application and for faster sample throughput with minimized sample volume. The technique is based on the flow injection method with solid phase extraction on a Sep-Pak C18 cartridge and colorimetric detector. The Schlieren effect was minimized by rinsing the cartridge sequentially with 5 mL water and 2 mL 95% ethanol solution. With three micro pumps in parallel, savings of up to 80% in amount of reagents and 25% volume of seawater samples could be achieved in comparison to the previous method. Variation of stopped flow time and sample loading time gave 3 different standard curves, which corresponded to 3 linear ranges within 3.4 and 515 nM. The modified method permits the analysis of samples over a wide range of concentrations, and has been successfully applied to shipboard determination of trace orthophosphate in more than 200 seawater samples during a one-month cruise in the South China Sea. For seawater at concentrations of 20.6, 82.5, 206.2 nM orthophosphate, the relative standard deviations (RSD) (n = 6), determined daily for 6 days on board ship were 4.45%, 4.73% and 6.75%, respectively. Five seawater samples collected in the Station SEATS (South East Asia Time Series Station at 18°N, 116°E) were analyzed using the present method both on board and in a land-based laboratory, as well as with the magnesium hydroxide-induced coprecipitation (MAGIC) method, and showed no significant difference according to the statistical t-test.     

BTEX异常对海域油气藏指示作用的研究

常规的油气化探方法一般是应用烷烃类作为油气勘探的基本指标,但是烷烃类易受人类活动和生物活动的干扰,使常规的化探技术在油气勘探中一直受到限制。人们开始考虑采用直接的生物标记化合物来预测油气藏,如芳烃系列中的苯系化合物苯(benzene)、甲苯(toluene)、乙苯(ethylbenzene)、二甲苯(xylem),简称BTEX。国内外研究表明,BTEX基本不受人类活动和生物活动的干扰,对油气藏具有良好的指示作用。依据南海某区的实测资料,采用自主研发的油气化探可视化评价软件进行数据处理,剥离背景异常,圈出有效的BTEX异常,并与靶区地层剖面进行了对比,结果证实了BTEX对海域油气藏具有良好的指示作用。     

金属电极在含油模拟海水中电化学行为的研究

采用电化学阻抗和恒电位阶跃技术研究了金属铂和镍电极在含油氯化钠溶液中的电化学行为特征,测量了不同含油量体系中电化学信号的变化,并建立了相应的等效电路.实验结果表明,同样条件下多孔电极比平面电极的电化学信号响应显著,泡沫镍电极与铂黑电极相比对含油量的响应则更为明显.     

海洋水色遥感图像伪彩化方法的研究

分析了国内外有关信息伪彩化应用的研究现状后认为:海洋水色遥感图像的伪彩化应该是基于任务的,也只有基于任务的伪彩化才能保证伪彩化结果的准确性。基于任务的伪彩化要求必须从伪彩化相关的3个对象(信息、色彩与用户)所具有的特点出发,确定有针对性的伪彩化实现方案。在综合考虑了伪彩化3个对象的特点后,提出海洋水色遥感图像的伪彩化可以分3种方案进行伪彩化设计,每种方案都有自己的针对性、使用范围及局限性。方案1是针对对海洋信息作定量分析而提出来的,主要用于揭示海洋信息中的高频快变成分,同时兼顾揭示信息中的低频成分,被分析信息的波动幅度不大。方案2是针对对海洋信息作定性分析而提出的,不强求对信息量的准确量化,更多关心的是海洋信息相互间的关系,因此要求色标具有很高的信息表现力。方案3是方案1及方案2的补充,方案1具有定量能力,但总色差较小,对信息的解析能力有限,方案2虽然有较大的总色差,具有较好的解析能力,但只有定性能力,方案3将在保持局部区域的定量特点外,扩大整体的总色差。在伪彩化或可视化设计中,主要是关心数据信息中最引人注意的部分也即突出最重要的部分,在大多数情况下,用户对数据信息的低端及高端更感兴趣,这也是方案3中双反色调色板设计及使用的基础。3种方案的针对性设计将方便伪彩化在水色遥感信息分析中的准确、快捷使用。文章最后通过例子证明,在对海洋水色遥感信息的分析和研究中,选择3个方案中的1种或几种作适当的调整,海洋水色遥感信息可以得到较好的诠释。     

海洋环境对猎雷声呐探测识别的影响及对策建议

猎雷是各海军强国重点发展方向,猎雷作战中首要的就是探测识别水雷。从传播损失、混响、 目标强度以及海流、潮汐等多个方面分析了海洋环境对猎雷声呐探测识别的影响,并从猎雷作战角度提出了有关参数设置、作战方式、数据处理等对策建议,可为部队探测识别水雷等目标提供有效参考。     

Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis

In crustaceans, the male sexual dif ferentiation and maintenance are specially regulated by androgenic gland(AG). However, little is known about the genes involved in the regulation process.RNA-Seq was performed on AG with ejaculatory duct(AG_ED) and ejaculatory duct(ED) as control in Eriocheir sinensis, one of the most important economic and ?shery crabs with typically sex dimorphism. A total of 925 unigenes were identi?ed as dif ferentially expressed genes(DEGs) and the expression of nine genes randomly selected was con?rmed by qRT-PCR. 667 unigenes were up-regulated in AG_ED, being supposed to be AG preferential genes. Among them, the full length of i nsulin-like androgenic gland factor( IAG) cDNA named as Es-IAG was obtained as a logo gene of AG, which together with the genes i nsulin-like receptor( INR), and s ingle insulin binding domain protein( SIBD), might constitute the sex regulation pathway. Several sex related genes were identi?ed, and their function will have to be investigated. Also,the identi?cation of j uvenile hormone epoxide hydrolase 1( JHEH1), ecdysteroid 22-hydroxylase( DIB) and e cdysone receptor( ECR) preliminarily clari?ed the molecular regulation mechanism of eyestalk-AG-testis axis, which plays important roles in molting and reproduction. The results will enhance our understanding for the molecular basis of the AG involved in male sex regulation in crabs.     

台湾海峡冬、夏季氮通量的数值模拟研究

本文建立了一个气候态驱动的台湾海峡物理-生态耦合模型（ROMS-NPZD）。与遥感观测数据的比较表明,模型能够较好地模拟出冬、夏季台湾海峡主要的温度和叶绿素分布特征。模型揭示了夏季台湾海峡营养盐输运的东、西通道,与南海次表层水的入侵通道一致;冬季,海峡中的营养盐来源于闽浙沿岸水和通过澎湖水道入侵的南海次表层水。模拟结果表明:夏季,通过海峡流入东海的氮主要为有机氮;冬季,闽浙沿岸流为海峡和南海北部陆架提供了丰富的营养盐,不仅如此,南海次表层水进入海峡的营养盐通量与夏季相当。