Initiation of primary cell culture from amphioxus <Emphasis Type="Italic">Branchiostoma belcheri tsingtauense</Emphasis>

Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C. Supported by Doctoral Initial Fund of Ludong University (No.43304)     

对秦岭造山带几个重大问题的认识

详细讨论了秦岭一大别造山带的几个重大问题，对它的发展阶段进行了新的划分，对其形成发表了新的观点：认为秦岭造山带、华北板块、扬子板块是中生代以来，在抽拉－逆冲岩片构造体制作用下形成的。对秦岭一大别造山带的表层构造特征以及它和深层构造的关系、花岗岩的成因类型、断层与界面的区分及成矿作用等都提出了和以往不同的认识。     

APPLICATION OF THE CLUSTERING METHOD IN ANALYSING SHALLOW WATER MASSES AND MODIFIED WATER MASSES IN THE HUANGHAI SEA AND EAST CHINA SEA

The existing various T-S diagram methods of analysing water masses have a good effect on the ocean water mass analysis, but they have many limitations in analysing water masses in the shallow sea. The main point is that it is difficult to determine the core of original water type in the shallow sea area, so a large error may be carried into the determination of the boundaries of water mass. For example, when water masses are analyzed with the conventional analysis method of concentration mixture, we usually obtain a very     

准噶尔盆地玛湖凹陷西斜坡三叠系百口泉组扇三角洲优质储集层预测

针对准噶尔盆地玛湖凹陷西斜坡风南地区三叠系百口泉组扇三角洲砂岩物性空间变化大、优质储集层(孔隙度大于7.4%,渗透率大于0.05×10^-3μm²)预测难的问题,在沉积岩石学、地震沉积学以及地震反演和解释理论指导下,综合利用测井、岩心和三维地震等资料开展了高精度层序地层划分、沉积微相描述和优质储集层地震反演研究。建立了风南井区四级层序地层格架,明确了扇三角洲多期水进水退的充填过程,指出SSQ3和SSQ5是优质储集层的发育层系;识别出扇三角洲平原分流河道、河道间和扇三角洲前缘水下分流水道、河口坝、席状砂等沉积微相,指出扇三角洲平原是优质储集层发育相带;通过应用高分辨层序地层纵向边界和沉积相横向边界约束,进行分层相控叠后地震波阻抗反演,提升储集层预测精度,在SSQ3和SSQ5预测5个优质储集层发育区,提出3口井的井位建议,钻探均获工业油流。     

Antiangiogenic activity of low-temperature lysozyme from a marine bacterium <Emphasis Type="Italic">in vivo</Emphasis> and <Emphasis Type="Italic">in vitro</Emphasis>

We extracted marine low-temperature lysozyme (MLTL), a novel lysozyme, from a marine microorganism through fermentation. Our previous study suggested that a low molecular weight (16 kDa) may exert anti-tumor activity through antiangiogenesis. In this study, we extracted a high weight (39 kDa) and investigated its antiangiogenic activity in vivo and in vitro. Using zebrafish embryos as an in vivo study model, we found that treatment with MLTL significantly inhibited the growth of subintestinal vessels (SIVs) in a dose-dependent manner and that 400 μg/ml MLTL was sufficient to block the growth of SIVs. An in vitro study conducted using human umbilical vein endothelial cells (HUVECs) revealed that MLTL suppressed the proliferation, migration and tube formation of HUVECs in a dose-dependent manner. Interestingly, assays by flow cytometry and DNA electrophoresis indicated that MLTL was able to induce apoptosis of HUVECs. Moreover, further study demonstrated that the disruption of intracellular Ca²⁺ homeostasis may play an important role in MLTL induced apoptosis of HUVECs. Taken together, the results of this study demonstrate for the first time that MLTL inhibits angiogenesis through its pleiotropic effects on vascular endothelial cells and induces apoptosis through regulation of cellular Ca²⁺ levels. The results of this study also revealed a possible mechanism underlying the antiangiogenic effect of MLTL and suggested that MLTL may be a promising new antiangiogenic agent for use in cancer therapy.     

Instruments, Detectors and the Future of Astronomy with Large Ground Based Telescopes

Results of a survey of instrumentation and detector systems, either currently deployed or planned for use at telescopes larger than 3.5 m, in ground based observatories world-wide, are presented. This survey revealed a number of instrumentation design trends at optical, near, and mid-infrared wavelengths. Some of the most prominent trends include the development of vastly larger optical detector systems (> 10⁹ pixels) than anything built to date, and the frequent use of mosaics of near-infrared detectors – something that was quite rare only a decade ago in astronomy. Some future science applications for detectors are then explored, in an attempt to build a bridge between current detectors and what will be needed to support the research ambitions of astronomers in the future.     

Satellite image analysis using crowdsourcing data for collaborative mapping: current and opportunities

ABSTRACT

Researchers are continually finding new applications of satellite images because of the growing number of high-resolution images with wide spatial coverage. However, the cost of these images is sometimes high, and their temporal resolution is relatively coarse. Crowdsourcing is an increasingly common source of data that takes advantage of local stakeholder knowledge and that provides a higher frequency of data. The complementarity of these two data sources suggests there is great potential for mutually beneficial integration. Unfortunately, there are still important gaps in crowdsourced satellite image analysis by means of crowdsourcing in areas such as land cover classification and emergency management. In this paper, we summarize recent efforts, and discuss the challenges and prospects of satellite image analysis for geospatial applications using crowdsourcing. Crowdsourcing can be used to improve satellite image analysis and satellite images can be used to organize crowdsourced efforts for collaborative mapping.     

现场快速分析技术及其在地质分析中的应用

现场分析是地质科学研究的重要工具。现场分析的内容主要包括野外地球化学分析、船上海洋资源分析、矿山污染监测、生态环境调查等。现场分析的形式大致可分为相对固定的现场实验室、船(车)载移动实验室、便携式仪器或其他小型器械等几种。该文对这三种类型的现场分析进行评述。     

PNN网络在预测MCS移动中的应用

神经网络是空间数据挖掘的一种重要手段。本文运用概率神经网络(PNN网络)对1998年夏季影响青藏高原上中尺度对流系统(MCS)东移的环境物理量场的空间分布特征进行了研究，得到了高原上MCS移动方向与环境物理量场空间分布之间的关系。研究表明，使用概率神经网络预测中尺度对流系统的移动具有较好的效果，从而为研究高原上MCS东移与环境场之间的关系提供了一种新的方法和思路。