Systematic study of effects of pH and ionic strength on attachment of phage PRD1

Objectives of this work are to investigate effects of pH and ionic strength (IS) on virus transport in saturated soil and to develop a quantitative relationship for these effects. A series of 50-cm column experiments with clean quartz sand under saturated conditions and with pH values of 5, 6, 7, 8, and IS values of 1, 10, and 20 mM were conducted. Bacteriophage PRD1 was used as a model virus. Applying a one-site kinetic model, attachment, detachment, and inactivation rate coefficients were determined from fitting breakthrough curves using the software package Hydrus-1D. Attachment rate coefficients increased with decreasing pH and increasing IS, in agreement with DLVO theory. Sticking efficiencies were calculated from the attachment rate coefficients and used to develop an empirical formula for sticking efficiency as a function of pH and IS. This relationship is applicable under unfavorable conditions for virus attachment. We compared sticking efficiencies predicted by the empirical formula with those from field and column experiments. Within the calibrated range of pH and IS, the predicted and observed sticking efficiencies are in reasonable agreement for bacteriophages PRD1 and MS2. However, the formula significantly overestimates sticking efficiencies for IS higher than 100 mM. In addition, it performs less well for viruses with different surface reactivity than PRD1 and MS2. Effects of pH and IS on detachment and inactivation rate coefficients were also investigated but the experimental results do not allow constraining these parameters with sufficient certainty.     

TIGRE: A new robotic spectroscopy telescope at Guanajuato,Mexico

TIGRE is a new robotic spectroscopy telescope located in central Mexico at the La Luz Observatory of the University of Guanajuato. The 1.2 m telescope is fiber‐coupled to an ´echelle spectrograph with a spectral resolving power exceeding 20000 over most of the covered spectral range between 3800 Å and 8800 Å, with a small gap of 130 Å around 5800 Å. TIGRE operates robotically, i.e. it (normally) carries out all observations without any human intervention, including, in particular, the target selection in any given observing night. In this paper we describe the properties of the TIGRE instrumentation and its technical realization, as well as our first operational experience with the performance and efficiency of the overall system. Finally, we present some examples of recent TIGRE observations. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

A regional response in mean westerly circulation and rainfall to projected climate warming over Tasmania, Australia

Coupled ocean–atmosphere general circulation models (GCMs) lack sufficient resolution to model the regional detail of changes to mean circulation and rainfall with projected climate warming. In this paper, changes in mean circulation and rainfall in GCMs are compared to those in a variable resolution regional climate model, the Conformal Cubic Atmospheric Model (CCAM), under a high greenhouse gas emissions scenario. The study site is Tasmania, Australia, which is positioned within the mid-latitude westerlies of the southern hemisphere. CCAM projects a different response in mean sea level pressure and mid-latitude westerly circulation to climate warming to the GCMs used as input, and shows greater regional detail of the boundaries between regions of increasing and decreasing rainfall. Changes in mean circulation dominate the mean rainfall response in western Tasmania, whereas changes to rainfall in the East Coast are less related to mean circulation changes. CCAM projects an amplification of the dominant westerly circulation over Tasmania and this amplifies the seasonal cycle of wet winters and dry summers in the west. There is a larger change in the strength than in the incidence of westerly circulation and rainfall events. We propose the regional climate model displays a more sensitive atmospheric response to the different rates of warming of land and sea than the GCMs as input. The regional variation in these results highlight the need for dynamical downscaling of coupled general circulation models to finely resolve the influence of mean circulation and boundaries between regions of projected increases and decreases in rainfall.     

On regional dynamical downscaling for the assessment and projection of temperature and precipitation extremes across Tasmania, Australia

The ability of an ensemble of six GCMs, downscaled to a 0.1° lat/lon grid using the Conformal Cubic Atmospheric Model over Tasmania, Australia, to simulate observed extreme temperature and precipitation climatologies and statewide trends is assessed for 1961–2009 using a suite of extreme indices. The downscaled simulations have high skill in reproducing extreme temperatures, with the majority of models reproducing the statewide averaged sign and magnitude of recent observed trends of increasing warm days and warm nights and decreasing frost days. The warm spell duration index is however underestimated, while variance is generally overrepresented in the extreme temperature range across most regions. The simulations show a lower level of skill in modelling the amplitude of the extreme precipitation indices such as very wet days, but simulate the observed spatial patterns and variability. In general, simulations of dry extreme precipitation indices are underestimated in dryer areas and wet extremes indices are underestimated in wetter areas. Using two SRES emissions scenarios, the simulations indicate a significant increase in warm nights compared to a slightly more moderate increase in warm days, and an increase in maximum 1- and 5-day precipitation intensities interspersed with longer consecutive dry spells across Tasmania during the twenty-first century.     

Biopolymer-biocement composite treatment for stabilisation of soil against both current and wave erosion

Acta Geotechnica - Increased frequency of extreme weather events has made the conservation of riverbanks and coastlines a global concern. Soil stabilisation via microbially induced calcite...     

Random porosity fields and their influence on the stability of granular media

It is well established that the mechanical behavior of granular media is strongly influenced by the media's microstructure. In this work, the influence of the microstructure is studied by integrating advances in the areas of geostatistics and computational plasticity, by spatially varying the porosity on samples of sand. In particular, geostatistical tools are used to characterize and simulate random porosity fields that are then fed into a nonlinear finite element model. The underlying effective mechanical response of the granular medium is governed by a newly developed elastoplastic model for sands, which readily incorporates spatial variability in the porosity field at the meso‐scale. The objective of this study is to assess the influence of heterogeneities in the porosity field on the stability of sand samples. One hundred and fifty isotropic and anisotropic samples of dense sand are failed under plane‐strain compression tests using Monte Carlo techniques. Results from parametric studies indicate that the axial strength of a specimen is affected by both the degree and orientation of anisotropy in heterogeneous porosity values with anisotropy orientation having a dominant effect, especially when the bands of high porosity are aligned with the natural orientation of shear banding in the specimen. Copyright © 2007 John Wiley & Sons, Ltd.     

Exploring an Ediacaran ‘nursery’: growth,ecology and evolution in a rangeomorph palaeocommunity

The recent discovery of a fossilized assemblage of juvenile Ediacaran rangeomorph macro‐organisms, fern‐like impressions from the Drook Formation of Newfoundland, suggests that the morphological diversity present within the earliest known communities of the Ediacaran biota was relatively high. The fledgling population of these Proterozoic organisms was smothered by volcanic material from a nearby eruption, preserving the standing community on the seafloor. As many as six different genera are identified, present less than three million years after the end of the Gaskiers glacial event. This hints at a hidden evolutionary history for these organisms that is yet to be discovered. Co‐occurring filamentous impressions, and other fossils interpreted to represent the remains of decayed and ‘effaced’ organisms, suggest that the bedding plane exhibits unusual ecological completeness, with the assemblage as a whole documenting an early example of secondary community succession. Discoveries such as this reveal the potential for high‐quality preservation of soft‐bodied Ediacaran organisms in siliciclastic successions, and provide valuable insights into rangeomorph development and palaeoecology.     

Computer simulation of chemical nucleation

The problem of nucleation at chemical instabilities is investigated by means of microscopic computer simulation. The first-order transition of interest involves a new kind of nucleation arising from chemical transformations rather than physical forces. Here it is the chemical state of matter, and not matter itself, which is spatially localized to form the nucleus for transition between different chemical states. First, the concepts of chemical instability, nonequilibrium phase transition, and dissipative structure are reviewed briefly. Then recently developed methods of reactive molecular dynamics are used to study chemical nucleation in a simple model chemical reaction. Finally, the connection of these studies to nucleation and condensation processes involving physical and chemical interactions is explored.Invited contribution to the Proceedings of a Workshop onThermodynamics and Kinetics of Dust Formation in the Space Medium held at the Lunar and Planetary Institute, Houston, 6–8 September, 1978.     

Evolution of the Janus-Epimetheus coorbital resonance due to torques from Saturn's ring

We analyze the interactions between Saturn's coorbital satellites, Janus and Epimetheus, and the outer edge of the A ring, which is presumably maintained by these moons at their 7:6 resonance. Using two distinct but conceptually related methods, we show that ring torques are driving these satellites into a tighter lock. Unless there is a counterbalancing force which we have neglected, their orbital configuration will evolve from the current horseshoe-type lock to one of tadpole orbits around a single Lagrange point in ～20 myr. This finding adds an additional member to the list of short time scale problems associated with the interactions between Saturn's rings and its inner moons     

Theoretical twin meteor showers

Attention is drawn to, and theoretical radiants calculated for, 27 hypothetical twin showers to Cook's (1973, in Evolutionary and Physical Properties of Meteoroids, NASA SP-319, U.S. Govt. Printing Office, Washington, D.C.) working list of meteor streams. Of these twin showers, 7 are previously known, 1 is a night-time twin to the ? Geminids, 9 are found to be contained among Sekanina's (1976, Icarus27, 265–321) radar streams, and the remaining 10 are undetected daytime showers. Minimum radii are computed for all of Cook's streams and are used to assess the possibility of detecting the twin showers.