An electrochemical RNA hybridization assay for detection of the fecal indicator bacterium Escherichia coli

Monitoring waters for indicator bacteria is required to protect the public from exposure to fecal pollution. Our proof-of-concept study describes a method for detecting fecal coliforms. The coliform Escherichia coli was used as a model fecal indicator. DNA probe-coated magnetic beads in combination with the electrochemical monitoring of the oxidation state of guanine nucleotides should allow for direct detection of bacterial RNA. To demonstrate this concept, we used voltammetry in connection with pencil electrodes to detect isolated E. coli 16S rRNA. Using this approach, 10(7) cells of E. coli were detected in a quantitative, reproducible fashion in 4h. Detection was achieved without a nucleic acid amplification step. The specificity of the assay for coliforms was demonstrated by testing against a panel of bacterial RNA. We also show that E. coli RNA can be detected directly from cell extracts. The method could be used for on-site detection and shows promise for adaptation into automated biosensors for water-quality monitoring.     

The chaotic rotation of Hyperion

A plot of spin rate versus orientation when Hyperion is at the pericenter of its orbit (surface of section) reveals a large chaotic zone surrounding the synchronous spin-orbit state of Hyperion, if the satellite is assumed to be rotating about a principal axis which is normal to its orbit plane. This means that Hyperion's rotation in this zone exhibits large, essentially random variations on a short time scale. The chaotic zone is so large that it surrounds the ½ and 2 states, and libration in the 3/2 state is not possible. Stability analysis shows that for libration in the synchronous and ½ states, the orientation of the spin axis normal to the orbit plane is unstable, whereas rotation in the 2 state is attitude stable. Rotation in the chaotic zone is also attitude unstable. A small deviation of the principal axis from the orbit normal leads to motion through all angles in both the chaotic zone and the attitude unstable libration regions. Measures of the exponential rate of separation of nearby trajectories in phase space (Lyapunov characteristic exponents) for these three-dimensional motions indicate the the tumbling is chaotic and not just a regular motion through large angles. As tidal dissipation drives Hyperion's spin toward a nearly synchronous value, Hyperion necessarily enters the large chaotic zone. At this point Hyperion becomes attitude unstable and begins to tumble. Capture from the chaotic state into the synchronous or ½ state is impossible since they are also attitude unstable. The 3/2 state does not exist. Capture into the stable 2 state is possible, but improbable. It is expected that Hyperion will be found tumbling chaotically.     

Ring torque on janus and the melting of Enceladus

Voyager 2 images show parts of Enceladus' surface to be very smooth, lacking craters down to the resolution limit of 4 km. This absence of craters indicates geologically recent resurfacing, probably due to internal melting. However, calculations of current heating mechanisms, including radioactive decay and tidal heating due to Enceladus' resonance with Dione, yield heating rates too small to cause melting. The orbital mean motion of Janus (1980S1) is slightly less than twice that of Enceladus and, according to theoretical calculations, is currently decreasing as Janus' orbit evolves outward due to resonant torques from Saturn's rings. If Janus were ever locked into a stable 2:1 orbital commensurability with Enceladus, the resulting angular momentum transfer could have sufficiently enhanced the eccentricity of Enceladus' orbit for the ensuing tidal heating to have melted Enceladus' interior. The existence of a Laplace-like three-body resonance including Dione, although unlikely, would have increased heating. If Janus were indeed held in resonance with Enceladus until recently (10⁷–10⁸ years B.P.) when the lock was disrupted by an unspecified event (possibly a catastrophic collision which simultaneously created the coorbital pair, or by the influence of Dione) both the recent internal activity of Enceladus and the proximity of Janus to Saturn's rings may be explained. However, the predicted rapid time scale for ring evolution due to resonant torques from Saturn's inner moons remains a major problem.     

A long-term Arctic snow depth record from Abisko, northern Sweden, 1913–2004

A newly digitized record of snow depth from the Abisko Scientific Research Station in northern Sweden covers the period 1913-present. Mean snow depths were taken from paper records of measurements made on a profile comprising 10 permanent stakes. This long-term record yields snow depths consistent with two other shorter term Abisko records: measurements made at another 10-stake profile (1974-present) and at a single stake (1956-present). The measurement interval is variable, ranging from daily to monthly, and there are no data for about half of the winter months in the period 1930-1956. To fill the gaps, we use a simple snowpack model driven by concurrent temperature and precipitation measurements at Abisko. Model snow depths are similar to observed; differences between the two records are comparable to those between profile and single stake measurements. For both model and observed snow depth records, the most statistically significant trend is in winter mean snow depths, amounting to an increase of about 2 cm or 5 % of the mean per decade over the whole measurement period, and 10% per decade since the 1930-40s, but all seasonal means of snow depth show positive trends on the longest timescales. However, the start, end, and length of the snow season do not show any statistically significant long-term trends. Finally, the relation between the Arctic Oscillation index and Abisko temperature, precipitation and snow depth is positive and highly significant, with the best correlations for winter.     

Phosphorus Cycling and Burial in Sediments of a Seasonally Hypoxic Marine Basin

Recycling of phosphorus (P) from sediments contributes to the development of bottom-water hypoxia in many coastal systems. Here, we present results of a year-long assessment of P dynamics in sediments of a seasonally hypoxic coastal marine basin (Lake Grevelingen, the Netherlands) in 2012. Sequential phosphorus extractions (SEDEX) and X-ray absorption spectroscopy (XAS) indicate that P was adsorbed to Fe-(III)-(oxyhydr)oxides when cable bacteria were active in the surface sediments in spring. With the onset of summer hypoxia, sulphide-induced dissolution of the Fe-(III)-(oxyhydr)oxides led to P release to the pore water and overlying water. The similarity in authigenic Ca-P concentrations in the sediment and suspended matter suggest that Ca-P is not formed in situ. The P burial efficiency was ≤ 32%. Hypoxia-driven sedimentary P recycling had a major impact on the water-column chemistry in the basin in 2012. Water-column monitoring data indicate up to ninefold higher surface water concentrations of phosphate in the basin in the late 1970s and a stronger hypoxia-driven seasonal P release from the sediment. The amplified release of P from the sediment in the past is attributed to the presence of a larger pool of Fe-bound P in the basin prior to the first onset of hypoxia. Given that P is not limiting, primary production in the basin has not been affected by the decadal changes in P availability and recycling over the past 40 years. The changes in P dynamics on decadal time scales were not recorded in sediment profiles of total P or organic C/total P.     

Evaluation of the CORDEX-Africa multi-RCM hindcast: systematic model errors

Monthly-mean precipitation, mean (T_AVG), maximum (T_MAX) and minimum (T_MIN) surface air temperatures, and cloudiness from the CORDEX-Africa regional climate model (RCM) hindcast experiment are evaluated for model skill and systematic biases. All RCMs simulate basic climatological features of these variables reasonably, but systematic biases also occur across these models. All RCMs show higher fidelity in simulating precipitation for the west part of Africa than for the east part, and for the tropics than for northern Sahara. Interannual variation in the wet season rainfall is better simulated for the western Sahel than for the Ethiopian Highlands. RCM skill is higher for T_AVG and T_MAX than for T_MIN, and regionally, for the subtropics than for the tropics. RCM skill in simulating cloudiness is generally lower than for precipitation or temperatures. For all variables, multi-model ensemble (ENS) generally outperforms individual models included in ENS. An overarching conclusion in this study is that some model biases vary systematically for regions, variables, and metrics, posing difficulties in defining a single representative index to measure model fidelity, especially for constructing ENS. This is an important concern in climate change impact assessment studies because most assessment models are run for specific regions/sectors with forcing data derived from model outputs. Thus, model evaluation and ENS construction must be performed separately for regions, variables, and metrics as required by specific analysis and/or assessments. Evaluations using multiple reference datasets reveal that cross-examination, quality control, and uncertainty estimates of reference data are crucial in model evaluations.