Bioaccumulation of trace metals in the Antarctic amphipod Orchomene plebs: evaluation of toxicokinetic models

Bioaccumulation of Cd, Co, Cu, Ni, Pb and Zn in Antarctic gammaridean amphipod collectives, Orchomene plebs (Hurley, 1965), was investigated during a cruise of RV "Polarstern" to the Wedell Sea. With the sole exception of Cd the organisms accumulated metals during exposure and depurated them in uncontaminated seawater. Four independent toxicokinetic experiments and one field study were modelled simultaneously to calculate the following size-dependent bioconcentration factors for organisms with body length 10 mm (BCF(10 mm)): 130 (Co), 4030 (Cu), 190 (Ni), 2900 (Pb), and 5210 (Zn). On the time scale of our experiments the data suggest an increased metal uptake by previously exposed test organisms. The collectives investigated may be regarded as potentially suitable biomonitors for Co, Cu, Ni, Pb and Zn but not for Cd. An approach to evaluate the sensitivity of Orchomene plebs as a biomonitor of waterborne metals in the field indicates minimal increments of the ambient exposure concentrations of 0.01 microg Co l(-1), 0.2 microg Cu l(-1), 0.4 microg Ni l(-1), 0.6 microg Pb l(-1) and 0.3 microg Zn l(-1).     

五种植物提取液的抗鱼病菌和抗鱼病毒效应(英文)

本文旨在探索用廉价的野生植物防治鱼病。所用的植物有牛繁缕(Stellaria aqu-atica),双花凤仙(Impatiens biflora),夜来香(Oenothera biennis),艾草(Artem-isia vulgaris)和忍冬(Lonicera japonica)。用作试验的鱼病菌共13种(18个菌株),分属于气单孢菌属、假单孢菌属、爱德华氏菌属、耶尔森氏菌属、弧菌属。所用的鱼病毒为传染性胰脏坏死病毒IPNV,传染性造血组织坏死病毒IHNV。上述病原微生物均获自美国马里兰大学微生物系。实验结果表明,五种植物提取液均有不同程度的抗鱼病菌作用,其中以牛繁缕的抗菌谱最广,作用力最强。忍冬、艾草、牛繁缕有抗鱼病毒作用。其中以忍冬的效果最好,它能抑制IPNV和IHNV。而艾草和牛繁缕只能抑制IHNV。     

Improved form of wind wave frequency spectrum

The lower frequency part of the theoretical wind wave spectrum proposed by the authors (Wen et al. , 1988a, b,c) has been improved and the form of spectrum is appreciably simplified. In addition to the field data collected in the Bohai Sea region and used in the previous papers, those obtained in the Huanghai Sea, the East China Sea and the South China Sea have been employed so that the improved spectra can be verified on a more extensive observational basis. Computed results agree with the observations well. Further comparisons have been made between the proposed spectra and the JONSWAP spectrum. Though the two types of spectrum are close to each other in form, the former shows, as a whole, better agreement with the observation than the latter. By introducing an improved relation between the peak-ness factor and significant wave steepness, the spectrum contains only significant wave height and period as parameters. For spectra given in this form, the computed peak frequencies coincide approximately wit     

Atmospheric Correction of SeaWiFS Data for the Retrieval of Suspended Sediment in East China Coastal Waters

The data of SeaWiFS (Sea-Viewing Wide Field-of-View Sensor), installed on SeaStar, has been used to generate SSC (suspended sediment concentration) of complex and turbid coastal waters in China. In view of the problems of the SeaDAS (SeaWiFS Data Analysis System) algorithm applied to China coastal waters, a new atmospheric correction algorithm is discussed, developed, and used for the SSC of East China coastal waters. The advantages of the new algorithm are described through the comparison of the restdts from different algorithms.     

Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes): comparison of gene expression and protein levels

Quantification of the egg yolk precursor vitellogenin (VTG) in fish has become a standard technique to detect estrogenic effects of known chemicals and environmental samples. In the present study, we have analysed VTG induction by estradiol, ethynylestradiol and genistein exposure in the model teleost medaka (Oryzias latipes) and demonstrate that the medaka is a suitable model system to analyse estrogenic effects. By comparing VTG gene expression and protein levels we show that in principal both techniques can be used to study VTG induction in vivo (juvenile and adult males) and in vitro (primary cultures of male liver cells). If a short term in vivo or in vitro exposure is performed, detection of mRNA might be sufficient. For long term studies with the need to detect weak estrogenic chemicals and a precise quantification, immuno-chemical detection may be favoured.     

Effects of organochlorine compounds on cytochrome P450 aromatase activity in an immortal sea turtle cell line

Many classes of environmental contaminants affect the reproductive function of animals through interactions with the endocrine system. The primary components affected by endocrine active compounds (EACs) are the steroid receptors and the enzymes responsible for steroidogenesis. This study sought to develop an in vitro model for assessing EAC effects in sea turtles by examining their ability to alter cytochrome P450 aromatase (CYP19) activity. Aromatase is the enzyme responsible for the conversion of testosterone to estradiol. This enzyme is critical in the sexual differentiation of reptiles which demonstrate temperature-dependent sex determination. An immortal testis cell line GST-TS from a green sea turtle was grown in culture at 30 degrees C in RPMI 1640 media. The cells were exposed to three known aromatase inducers; dexamethasone (Dex), 8Br-cyclic AMP, or human chronic gonadotropin (HCG) and one aromatase inhibitor 4-androstenol-dione (4-OHA). In addition, the GST-TS cells were exposed to 0.1-30 microM atrazine and 3-100 microM 4,4'-DDE. The inducing compounds that have been shown to increase aromatase activity in other systems failed to induce aromatase activity in the GST-TS cells, yet exposure to the inhibiting compound, 4-OHA, did result in a significant reduction. Atrazine (0.1, 1.0 and 10 microM) significantly induced aromatase activity following a 24 h exposure, and 4,4'-DDE inhibited the activity but only at cytotoxic concentrations (100 microM). Based on these results, this in vitro model can be useful in examining the endocrine effects of EACs in sea turtles.     

白垩纪温度较冷的估价

白垩纪温度较冷的估价Ｂ．ｗ．Ｓｅｌｌｗｏｏｄ等有关白垩纪气候的概念部分基于详尽的地质证据，如不耐寒冷的古生物（如：珊瑚的分布比现在偏极地５－１５℃）和特殊气候条件的沉积相的广泛分布。这些资料的解释反映２１℃的等温线在纬度上至少向两极移动５°，全球的平...     

Molecular identification of bloom-forming species Phaeocystis globosa(Prymnesiophyta)and its dispersal based on rDNA ITS sequence analysis

Abstract-The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associatedwith their colonial stages. In this study we applied molecular approaches to analyze the genetic variationof Phaeocystis globosa and Phaeocystis pouchetii from several geogrtaphic regions, and to assist in tracingthe dispersal of bloom-forming Phaeocystis species in coastal waters of China. The Sequences of the inter-nal transcribed sacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystisstrains were determined. Sequence comparison shows that P.globosa was the most divergent toP. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences ex-isted between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearlyshows that the species isolated from the southeast coast of China is identified as P. globosa rather thanP. cf. po     

Using benthic macrofauna to assess environmental quality of four intertidal mudflats in Hong Kong and Shenzhen Coast

Abstract-Intertidal zone is a significant wetland between land and ocean. It plays an important rolein maintaining local ecological balance. Both Mai Po and Futian intertidal mudflats are located in Shen-zhen Bay and are important "refueling" point along the East Asian/Australian flyway of migratory birds.The environmental quality of Mai Po and Futian mudflats have aroused great concern due to rapid eco-nomic developments in Hong Kong and Shenzhen in recent decades. Microinfauna of Mai Po and Futianmudflats was investigated in December 2000 and the faunal data were used to assess their environmentalquality. Two other mudflats, namely Ma Wan Typhoon Shelter (a more disturbed area) and Luk Kengintertidal mudflat (a place with relatively less human disturbance), were also sampled for macroinfaunaand used as reference mudflats. Shannon-Weaver species diversity index (I_(sd)), biotic coefficient (C_b)and macrofaunal pollution index (I_(mp)) of the macrofauna community on four intertidal mudflats wereused to