Seasonal variation in lysosomal destabilization in oysters,Crassostrea virginica

Lysosomal destabilization assays have been used as valuable biomarkers of pollutant exposures in a variety of bivalve and fish species. The responses of oysters, Crassostrea virginica, deployed at and native to various reference and degraded sites were evaluated for lysosomal destabilization during both summer and winter seasons. In both native and deployed oysters, lysosomal destabilization rates tended to be higher during the winter at both reference and polluted sites. There are at least two hypothetical explanations. Greater lysosomal destabilization rates may be related to physiological changes associated with mobilization of nutrient reserves during the winter and gametogenesis. However, lysosomal destabilization in deployed oysters was correlated with tissue metal concentrations. These data also support a second hypothesis that seasonal differences in physico-chemical factors (such as reduced levels of acid volatile sulfides) may increase the bioavailability of metals during the winter so that adverse effects are more pronounced.     

电控计时部分收集器

部分收集器具有自动定量分部收集的功能,是柱层析技术中的重要仪器,它不仅可用于生物化学和分析化学中,而旦在制药、化工等方面的分离工作中,也有使用价值。目前国内外一般是按计时间、计体积、计重量或计滴数而设计制造的。 我们在研究海洋生物氨基酸含量工作过程     

吸收式造波机和宽式波浪槽

介绍一种宽式波浪槽，槽中设有一组吸收式造波机，它能吸收反射波。     

渤海三维潮流数值模拟

本模型以具有自由面的三维非线性Ｎａｖｉｅｒ－Ｓｒｏｋｅｓ方程为基本方程，经σ坐标变换得到模式方程。基于过程分裂法，将三维流动中的快过程与慢过程劈开，并对每部分选用适合其物理特性和数值行为的计算格式，然后耦联求解。应用本模型详细计算了渤海的三维潮运动。其中，潮波系统模拟获得理想的结果；采用十层模式计算了Ｍ２潮流，以较高的辨率揭示了潮流的空间结构特征。最后本文给出了三维潮流预报的应用实例。     

Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of heavy metal exposure

Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.     

第23章 DNS模式:一种为大洋波浪预报而改进的谱模式

23.1 介绍 模拟与频率和方向相关的大洋波谱F（f,θ）的工作是极其困难的。唯一有点统一的理论（Hasselmann,1967）也还没有简化成一种实用形式。这篇研究中所探讨的是结合零散的,现有的理论,以达到这样一种目的:即发展一种与我们目前对风浪成长谱的了解相一致 ·95.的波谱模式。本章中我们将讨论一种离散的谱模式,这种模式采用了一种改进的能量传播的特征方法。数值方法最早是由Barnett等（1969）提出的。     

青岛近海风暴潮灾害及其预报研究

本文采用周期外推法预报增水峰时，采用回归计算和增水峰连线外延相结合法预报峰值，然后以此模拟增水曲线对台风在人任意２４ｈ之内增水进行过程预报，效果较好。