Isotopic variation of fishes in freshwater and estuarine zones of a large subtropical coastal lagoon

We used stable C and N isotope ratios of tissues from 29 fish species from a large subtropical lagoon in southern Brazil to examine spatial variability in isotopic composition and vertical trophic structure across freshwater and estuarine habitats. Nitrogen isotope ratios indicated a smooth gradation in trophic positions among species, with most fishes occupying the secondary and tertiary consumer level. Fish assemblages showed a significant shift in their carbon isotopic signatures between freshwater and estuarine sites. Depleted carbon signatures (from −24.7‰ to −17.8‰) were found in freshwater, whereas more enriched signatures (from −19.1‰ to −12.3‰) were obtained within the estuarine zone downstream. Based on our survey of the C₃ and C₄ plants and isotopic values for phytoplankton and benthic microalgae reported for ecosystems elsewhere, we hypothesized that the observed δ¹³C differences in the fish assemblage between freshwater and estuarine sites is due to a shift from assimilating organic matter ultimately derived from C₃ freshwater marsh vegetation and phytoplankton at the freshwater site (δ¹³C ranging from −25‰ to −19‰), to C₄ salt-marsh (e.g. Spartina) and widgeon grass (Ruppia maritima), benthic microalgae and marine phytoplankton at the estuarine sites (from −18‰ to −12‰). Our results suggested that fish assemblages are generally supported by autochthonous primary production. Freshwater fishes that likely were displaced downstream into the estuary during periods of high freshwater discharge had depleted δ¹³C values that were characteristic of the upper lagoon. These results suggest that spatial foodweb subsidies can occur within the lagoon.     

Observations of a fast burst of the deep western boundary undercurrent and sediment transport in South Wilmington Canyon from DSRVAlvin

On October 3 and 4, 1986, DSRVAlvin dives encountered a strong current at 2,300 m in South Wilmington Canyon. The current, estimated at 1 knot, transported surficial sediment and constructed and modified bedforms. It appears to have been constant in its direction of flow from 30 to 40°. The observed current was probably a burst of fast flow in a region of slow average currents in the Deep Western Boundary Undercurrent. Such episodic events may have a greater influence on the stratigraphic record than the temporally longer more tranquil flow conditions.     

Response of the suspended sediment transport system to continental shelf dynamics

Surface currents influenced by a wind-driven upwelling event in San Pedro Bay moved total suspended matter (TSM) confined to the inner shelf on 19 April 1978 seaward, so that by 27 April surface TSM had increased over the outer shelf. Near-bottom concentrations of TSM also increased across the shelf during this time. This is explained by sediment resuspended by large surface waves being advected from the inner shelf seaward by the mean flow after this flow had turned from southeasterly to southerly when upwelling ceased on 26 April. These complex shelf dynamics contribute to the off-shelf transport of mud to the slope and deep basins.     

Variations in the Rate of Anaerobic Succinate Accumulation within the Central and Marginal Regions of an Euryoxic Bivalve Mantle

Abstract. Anaerobic metabolism in the central and marginal portions of the mantle of Mercenaria mercenaria was compared. Anaerobic succinate accumulation was more rapid in the central region. This difference may be due to higher phosphoenolpyruvate carboxykinase activity in the central region. Thus, the central region is more specialized for anaerobic metabolism and the marginal region more for net shell growth. The original rate of succinate accumulation in the mantle is similar in isolated mantles and intact clams, suggesting that mantle succinate production does not require translocation of precursors from other tissues. However, in intact clams, the rate of succinate accumulation in the central region of the mantle slows after four hours. The reduced rate is probably caused by reducing the metabolic rate. Succinate accumulation and shell dissolution are slower in freshly collected clams than in clams that had been stored anaerobically. The difference may be due to induction of PEPCK synthesis during storage. Shell derived calcium did not accumulate in the mantle and, therefore did not alter the intracellular calcium concentration in the mantle.     

Carbon dioxide partial pressures in North Pacific surface waters — Time variations

Observations were made of time variations of the carbon dioxide partial pressures (Pco₂) of the atmosphere and surface sea waters in the Pacific subarctic region. Data were obtained on a cruise of the USC & GSSSURVEYOR in October, 1968 and on the TRANSPAC expedition of the CNAVENDEAVOUR in March–April, 1969. A rise in surface water Pco₂ of 18×10^–6 atm occurred in a period of 30–45 days in March–April due principally to spring warming of surface waters. An average increase of 60×10^–6 atm occurred between October, 1968 and March, 1969 as a result mainly of cessation of summer phytoplankton production and the onset of winter-storm-driven vertical mixing. Because the air-sea Pco₂ gradient not only changed appreciably in magnitude but also changed sign, there are important implications for calculations of air-sea exchange of carbon dioxide on the ocean wide scale.Data contained in this paper comprise part of a dissertation to be submitted by Louis I. Gordon in partial fulfillment of the requirements for the Ph. D. at Oregon State University.     

The estimation of acid dissociation constants in seawater media from potentionmetric titrations with strong base. I. The ionic product of water — Kw

The various assumptions implicit in the calculation of acid dissociation constants (based on ionic medium standard states) from potentiometric titrations using a cell with liquid junction (i.e. a pH measuring cell) have been examined. It was concluded that results can be obtained having an accuracy commensurate with the experimental precision. It has been shown that although the precise composition of the medium is a function of the hydrogen ion concentration (because of the protolytic nature of some of the ions in the media, e.g., sulphate and fluoride), the effect of such variations in the medium composition can be compensated for when defining the activity of hydrogen ion on an ionic medium standard state by defining the concentration of hydrogen ion as:

where β_HSO4 and β_HF are the relevant association constants and S_T and F_T are the total concentrations of sulphate and fluoride, respectively.This approach was used to obtain values for the ionic product of water (K_W) in artificial seawater media at various temperatures and ionic strengths. These were fitted to give the equation (molal concentration units):

$\text{pK}{}_{\mathrm{w}}\text{=}\text{3441.0}\text{T}\text{+2.256-0.7091}{}^{\mathrm{<mtext>1</mtext><mtext>2</mtext>}}\text{(}\text{rms deviation 0.01}\text{)}$

where I is the formal ionic strength of the artificial seawater medium and T is the absolute temperature. The values obtained are in reasonable agreement with those found by previous workers.     

Assessment of imposex in the common whelk, Buccinum undatum (L.) from the Eastern Scheldt, The Netherlands

Several cruises were carried out on the Eastern Scheldt between September 1992 and March 1995 to study the frequency and appearance of imposex in the common whelk, Buccinum undatum L. Incidences of imposex were always > 90%. For the first time several stages of imposex in Buccinum undatum are reported. The evolution of imposex in the whelk seems to follow a similar route as in Hinia reticulata belonging to the same superfamily (Buccinacea). Tissue organotin concentrations of animals collected in February 1995 showed the highest organotin concentrations in the digestive gland and lowest in the foot of the animals. Concentrations of phenyltin compounds (up to 625 ngSn/g DW) were much higher than those of butyltin compounds (up to 40 ngSn/g DW).     

A digital event-recording ocean bottom seismometer capsule

The ocean bottom seismometer capsule contains a 1 Hz. vertical seismometer and triggerable or programmable digital recording system. The output of the seismometer is continuously digitized at a preselected rate of 64, 128, or 256 samples/sec. The digital data words are mixed with a time code and synchronization characters, serialized and passed through a 1536 sample shift register which acts as a delay line. The serial output bits are then encoded and recorded on a SONY TC800B tape recorder which is turned on when a seismic event occurs. The event trigger occurs when the seismic signal jumps to 8 times the time averaged input signal. A memory may be programmed to run the recorder on a schedule so that small amplitude signals from refraction shots are sure to be recorded. Data are recovered using the same recorder for playback and a decoder which provides an analog output for field data interpretation or a digital output for computer analysis. An acoustic transponder allows precise ranges between the capsule and ship to be determined. In addition, commands for the capsule to release or to transmit diagnostic data may be given from the surface ship. The capsule falls freely to the ocean bottom. After a predetermined time or when a release command is received, it is released from a 68 kg steel tripod and floats to the surface. A dual timer and explosive bolt system is used to increase recovery reliability.The first capsules were designed and constructed between October 1972 and October 1973. Good results were obtained from 38 out of 43 launchings made on six expeditions in 1974, 1975, and 1976. Four capsules have been lost.     

Metabolism of perylene by monooxygenases of the skin and liver of Ambystoma tigrinum

The prevalence of dermal neoplasia among neotenic tiger salamanders (Ambystoma tigrinum) collected from the Reese Air Force Base sewage lagoon far exceeded that among salamanders from nearby uncontaminated lagoons. Perylene was the predominant pglycyclic aromatic hydrocarbon contaminant in this lagoon. Perylene was shown to be a substrate for monooxygenases in skin and liver homogenates and formed DNA-binding metabolites. Perylene exposure appeared to augment subsequent perylene metabolism by dermal enzymes but it was not a strong inducer of hepatic monooxygenases. Aroclor 1254 induced benzo(a)pyrene and perylene metabolism by both skin and liver enzymes. However, perylene apparently was not activated by salamander enzymes, as suggested by its lack of in vitro mutagenicity and failure to induce tumors.