Application of rotifer Brachionus plicatilis in detecting the toxicity of harmful algae

The toxicity of seven major HAB (harmful algal bloom) species/strains, Prorocentrum donghaiense, Phaeocystis globosa, Prorocentrum micans, Alexandrium tamarense (AT-6, non-PSP producer), Alexandrium lusitanicum, Alexandrum tamarense (ATHK) and Heterosigma akashiwo were studied against rotifer Brachionus plicatilis under laboratory conditions. The results show that P. donghaiense, P. globosa, P. micans, A. tamarense (AT-6), or A. lusitanicum could maintain the individual survival and reproduction, as well as the population increase of the rotifer, but the individual reproduction would decrease when exposed to these five algae at higher densities for nine days; H. akashiwo could decrease the individual survival and reproduction, as well as population increase of the rotifer, which is similar to that of the starvation group, indicating that starvation might be its one lethal factor except for the algal toxins; A. tamarense (ATHK) has strong lethal effect on the rotifer with 48h LC₅₀ at 800 cells/mL. The experiment on ingestion ability indicated by gut pigment change shows that P. donghaiense, P. globosa, P. micans, A. tamarense (AT-6) and A. lusitanicum can be taken by the rotifers as food, but A. tamarense (ATHK) or H. akashiwo can be ingested by the rotifers. The results indicate that all the indexes of individual survival and reproduction, population increase, gut pigment change of the rotifers are good and convenient to be used to reflect the toxicities of HAB species. Therefore, rotifer is suggested as one of the toxicity testing organisms in detecting the toxicity of harmful algae. Supported by the Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-YW-208), National Natural Science Foundation of China (No. 40821004, U0733006) and National Basic Research Program of China (973 Program, No. 2001CB409700)     

Development of a new index for integrating landscape patterns with ecological processes at watershed scale

Understanding the relationship between landscape patterns and ecological processes has been a central yet challenging research theme in landscape ecology. Over the past decades, many landscape metrics have been proposed but few directly incorporated ecological processes. In this paper, we developed a landscape index, namely, location-weighted landscape index (LWLI) to highlight the role of landscape type in ecological processes, such as nutrient losses and soil erosion. Within the framework of the Lorenz curve theory, we develop this index by integrating landscape pattern and point-based measurements at a watershed scale. The index can be used to characterize the contribution of landscape pattern to ecological processes (e.g. nutrient losses) with respect to a specific monitoring point in a watershed. Through a case study on nutrient losses in an agricultural area in northeastern China, we found that nutrient losses tended to be higher for a watershed with a higher LWLI value, and vice versa. It implied that LWLI can be used to evaluate the potential risk of nutrient losses or soil erosion by comparing their values across watersheds. In addition, this index can be extended to characterize ecological processes, such as the effect of landscape pattern on wildlife inhabitation and urban heat island effect. Finally, we discuss several problems that should be paid attention to when applying this index to a heterogeneous landscape site.     

基于平板型Web论坛的信息抽取研究

Web论坛信息抽取是Web论坛分析的预处理步骤.在舆情分析研究中,论坛帖子的各种属性是分析舆论话题演变的重要数据;由于平板型网络论坛页面的结构,使帖子的回复对象属性抽取较难实现.通过对平板型网络论坛结构及论坛用户网络发帖习惯的研究,针对平板结构网络论坛提出了一个抽取方案,实现了对帖子的各种属性的信息抽取.     

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胶南市七宝山铅矿电法测量适宜性探讨

胶南七宝山铅矿位于胶南造山带北缘,是在该造山带上首次发现的铅工业矿床。矿体呈脉状赋存于构造裂隙中,为含银铅萤石石英脉,伴生萤石、银,部分地段伴生铜。大功率激电测深和可控源音频大地电磁测深在该区试验有一定效果,对寻找地下盲矿体、推断矿体倾向延深有一定帮助,有效地指导了钻探工程的部署,在相应的异常部位找到了深部矿体,电法勘探显示了一定的找矿效果。     

GPS技术在山东省大地测量中的应用

全球定位系统(Global Positioning System-GPS)是美国国防部于20世纪70年代初开始设计、研制并于1993年底建成的军民两用卫星导航定位系统.它的建立不仅使导航技术和定位技术产生了根本变革,且对空间技术、军事、地学研究、交通运输等诸多领域及社会生活的各个方面都产生了重大影响.GPS技术在测量领域中的应用,使平面控制测量手段进入到一个崭新的时代.     

我对储量分类中[三维框架]的理解

目前,专家对怎么修订和完善《固体矿产资源／储量分类》意见尚不统一。但基本认同在保持联合国分类框架（亦即我国1999年的分类框架）的基础上进行调整,即对地质轴和可研轴可基本维持原状,主要是对经济轴进行调整。笔者认为,国际分类和我国的分类其核心都是三维框架,但是对三维框架的理解是不一致的,笔者的理解是：     

兖州市采煤塌陷地综合治理的几点做法

兖州地处鲁西南大平原,交通便利,矿产资源丰富,是全国八大煤炭基地之一,境内有煤矿4个,截至目前因采煤造成土地塌陷2000余公顷,常年积水660余公顷,平均水深4m,绝产面积达1200 hm2,并以每年130余公顷的速度增加.     

可用于相关亚历山大藻(Alexandrium affine)检测的一个寡核苷酸探针

针对分离自南海的相关亚历山大藻,在进行核糖体大亚基DNA（LSU rDNA）全序列测序及比对的基础上,设计了针对其核糖体大亚基RNA（LSU rRNA）D10区的一个新的荧光素（FITC）标记寡核苷酸探针,并建立了相应的荧光原位杂交检测方法。在GenBank数据库中,BLAST检索的结果显示所设计的探针具有较高的特异性。杂交实验的结果也表明,这条探针对相关亚历山大藻的标记具有特异性,且标记效率可以达到100％,标记信号清晰明亮。本研究结果为相关亚历山大藻的监测及其种群动态研究提供了方法学依据,也为其它微藻特异性探针的设计提供了新的思路。