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61.
于1992年1月-1993年2月,对从海湾扇贝中提取获得的糖胺聚糖(GAG)用乙醇和溴化十六烷基三甲铵(CTAB)分级分离,并对所得制品用琼脂糖凝胶电泳和高效液相色谱验证,以研究一种简便、快速的分离提纯方法。结果表明,扇贝GAG水溶液在乙醇量60%时分离获得的级分,其GAG-GTAB给合物再用2.0 mol/LNaCl溶液解离得到的主要级分,琼脂糖凝胶电泳和高效液相色谱均显示出单一区带,红外光谱类似干肝素的。证明这一提纯过程是一简便、快速的分离提纯方法。  相似文献   
62.
中国对虾染色体制备及染色体形态的研究   总被引:6,自引:2,他引:6  
以中国对虾原肠期胚胎为材料,采用整体低渗法和管渗法均制备了染色体。通过比较发现,管渗法制备的染色体比整体低渗法的形态好。本文对制备中国对虾染色体技术方面的问题进行了探讨。通过作者的观察,了解到中国对虾染色体徐正常特征外,还存在较特别的形态特征,如主缢痕增强,次缢痕变化,同源配对等。并对产生这些形态特征的原因进行了解释。  相似文献   
63.
中国沿海星虫动物门名录   总被引:2,自引:0,他引:2  
取1980~1989年间在我国沿海进行星虫动物区系调查的标本和有关资料做了整理、鉴定,共39种,分隶于2纲,4目,6科,12属。其中1种在我国为首次记录。  相似文献   
64.
In this paper, the responses of the interaction system of R.C. gravity single-leg platform to seismic excitation are mainly analysed. A set of nonlinear equations for the interaction system are established by using the wave, one is the soil-structure interaction and the other is the fluid-structure interaction. The seismic response of the interaction system is analysed for the influence of the asymmetric structure, fluid action, etc. with the input of seismic SH waves in any direction. The numerical results are given for a simple example.  相似文献   
65.
A new approach to high-order Boussinesq-type equations with ambient currents is presented. The current velocity is assumed to be uniform over depth and of the same magnitude as the shallow water wave celerity. The wave velocity field is expressed in terms of the horizontal and vertical wave velocity components at an arbitrary water depth level. Linear operators are introduced to improve the accuracy of the kinematic condition at the sea bottom. The dynamic and kinematic conditions at the free surface are expressed in terms of wave velocity variables defined directly on the free surface. The new equations provide high accuracy of linear properties as well as nonlinear properties from shallow to deep water, and extend the applicable range of relative water depth in the case of opposing currents.  相似文献   
66.
1 .IntroductionTheBohaiGulfisinfestedbyiceinthewholewinter,andoffshorestructuresthereexperiencenotonlywind ,waves ,currentsandearthquake ,butalsoiceloadswhicharecomplicatedbecauseofthedynamicinteractionbetweenicesheetsandstructures.Duanetal.( 2 0 0 0 )hadacomprehensivere viewofthelatestadvancesonice inducedvibration ,fatigueandfractureofoffshorestructures ,payingmuchattentiontothedifferencesinicebreakingmodeanddynamicsofstructuresindifferentice in festedwaters .Theypresentedcriticallythelates…  相似文献   
67.
The surface and bottom waters samples were collected from six locations in Xiamen western sea. The quantified estimation of bacterial production (3H-thymidine method) and observation of bacterial heterotrophic activity (14C-glucose method) have been made in order to have a better understanding of the role of marine bacteria and their activities. The results showed that the mean value of bacterial heterotrophic activity was 9×108 cells/(L.h) in the surface waters and 2.6×108 cells/(L.h) in the bottom waters. The mean value of bacterial production was 38×108 cells/(L.h) in the surface waters and 7.1×108 cells/(L.h) in the bottom waters. The relationship between bacterial production, heterotrophic activity, POC and DOC measured during this survey were discussed. The good understanding of the relationship between bacteria activity and total coliform was addressed.  相似文献   
68.
厦门港浮游植物对磷酸盐吸收速率的研究   总被引:12,自引:1,他引:12  
用无载体32P同位素示踪测定海水中浮游植物和细菌吸收磷酸盐速率的方法,对厦门港浮游植物和细菌进行了研究。结果表明,厦门港海水中吸收磷酸盐的主体是浮游植物(几乎占100%),平均吸收速率为4.28×10-5μmol/(L·s),并且存在着晚秋<冬<初春的季节变化,培养实验的结果表明吸收速率的季节变化主要是由于种类更替和温度效应所致。  相似文献   
69.
- The wave-current forces on vertical piles in side-by-side arrangement induced by irregular waves with opposing current are investigated experimentally in this paper. The characteristics in both time and frequency domain of in-line, lift and resultant forces are analyzed. The grouping effect coefficients of inline, lift and resultant forces on piles related to KC number and relative spacing parameters are given. These results are compared with those in the case of irregular waves combined with following currents. It is found that the results in these two cases are quite different. The range of KC number tested is 10- 60, the range of Reynolds number is (0.55-3.43) ×104.  相似文献   
70.
A Computational Model for Velocity Separation in Shallow Sea   总被引:1,自引:0,他引:1  
SONG  Zhiyao 《中国海洋工程》2002,16(3):407-413
Based on the hydrodynamical feature and the theoretical velocity profiles of tidal flow and vvind-induced flow in shal-low sea, a computational model is established for the first time, which can separate observed velocity into tidal velocity and wind-induced velocity by use of the least square method. With the model, not only the surface velocities of tidal flow and vvind-induced flow are obtained, but also the bed roughness height is determined and the wind velocity above the wa-ter surface is estimated. For verification of the model, the observed velocity in the Yellow River Estuary and the laborato-ry test is separated, then it is applied to the Yangtze River Estuary. All the results are satisfactory. The research results show that the model is simple in method, feasible in process and reasonable in result. The model is a valid approach to analysis and computation of field dala, and can be applied to separate the observed velocity in shallow sea; at the same time, reasonable boundary conditions of th  相似文献   
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