Development of three multiplex PCR primer sets for ark shell (<Emphasis Type="Italic">Scapharca broughtonii</Emphasis>) and their validation in parentage assignment

Scapharca broughtonii is a commercially important and over-exploited species. In order to investigate its genetic diversity and population structure, 43 novel polymorphic microsatellites were isolated and characterized. The number of alleles per locus ranged from 3 to 22 with an average of 6.93, and the observed and expected heterozygosities varied between 0.233 and 1.000, and 0.250 and 0.953, with an average of 0.614 and 0.707, respectively. Three highly informative multiplex PCRs were developed from nine of those microsatellites for S. broughtonii. We evaluated and validated these multiplex PCRs in 8 full-sib families. The average polymorphism information content (PIC) was 0.539. The frequency of null alleles was estimated as 3.13% of all the alleles segregation based on a within-family analysis of Mendelian segregation patterns. Parentage analysis of real offspring demonstrated that 100% of all offspring were unambiguously allocated to a pair of parents based on 3 multiplex sets. Those 43 microsatellite loci with high variability will be helpful for the analysis of population genetics and conservation of wild stock of S. broughtonii. The 3 sets of multiplex PCRs could be an important tool of pedigree reconstruction, population genetic analysis and brood stock management.     

渤海湾海岸带地区~(137)Cs参考剖面——意义、方法及初步结果

海岸带地区近百年来的现代地质过程重建必须以精确的年代学研究为基础,~(137)Cs时标法和~(210)Pb_(exc)测年法是目前广泛应用的方法。由于易受到河流沉积物供给变化、潮位状况、极端天气事件等的影响,海岸带现代过程的定量研究一直是高分辨率研究的瓶颈。区域性参考剖面可以提升现代沉积物测年方法在海岸带应用中的可靠性。因此,建立区域性的~(210)Pb_(exc)和~(137)Cs比活度-深度参考剖面,将改善对海岸带及邻近海区实测数据的解释。以渤海湾海岸带为研究区,选取沉积环境相对稳定、水平搬运作用较小的地区,采用人工探坑、Eijkelkamp槽型取样器获取了2个柱状岩心,通过γ能谱仪对样品进行~(210)Pb、~(226)Ra及~(137)Cs比活度测试,绘制剖面图,结合研究区已有数据,总结出6类海岸带常见的~(210)Pb_(exc)和~(137)Cs比活度-深度剖面类型,并重建了渤海湾海岸带~(137)Cs区域性参考剖面。结果显示,~(137)Cs在渤海湾地区的最大峰值指示1963年,可以作为区域性主要参考时标。但是,最大峰值上部的次峰在不同区域指示不同的时标,S4和S5两个站位该峰值指示的并非1986年时标。     

莱州湾南岸全新世相对海平面变化重建

在对莱州湾南岸8个钻孔沉积物沉积结构及有孔虫特征分析基础上,识别相关海面标志层位,辅以加速器质谱AMS14C测年,重建了全新世相对海面变化历史,并讨论了海面变化的沉积响应及控制因素。约9200cal BP以前,海面快速上升,研究区海侵时海面于-21.5m左右;9200~8400cal BP海面上升速率减缓至约2mm/a;8400~8000cal BP海面由-14m快速上升至-5.5m,速率约为33mm/a;8000~7600cal BP,海面持续数百年停滞或微弱下降;7600~7000cal BP海面由-5.5m快速上升至0m以上,速率至少约为13mm/a;7000~6000cal BP海面缓慢上升至+2~+3m位置,速率约为3mm/a;约6000cal BP以后海面缓慢下降至现今水平。约9200cal BP以前、8400~8000cal BP、7600~7000cal BP时期的3次海面快速上升,是MWP-1C融水脉冲、诱发8.2ka冷事件的融水脉冲,以及MWP-2融水脉冲的中纬度地区响应。中全新世全球冰融趋于停滞后,由于研究区沉积盆地沉降速度较慢,在冰川均衡调整效应下,使+2~+3m的相对高海面得以呈现。     

北京密云放马峪基性岩墙群LA-MC-ICP-MS锆石U-Pb年龄及其意义

太古宙基性岩墙群在北京密云地区广泛分布,其年代学研究一直备受关注,多年来取得了一些数据,但对太古宙变质片麻岩控制作用未做论述。利用LA-MC-ICP-MS U-Pb同位素测定技术,测得放马峪石榴辉石岩中锆石的年龄为2497±15Ma,此年龄被解释为区内变质片麻岩成岩年龄应早于基性岩墙群成岩年龄,其为研究区内太古宙片麻岩成岩年龄提供了重要的参考数据。     

Responses of meiofauna and nematode communities to crude oil contamination in a laboratory microcosm experiment

We examined the effects of crude oil contamination on community assemblages of meiofauna and nematodes after exposure to total petroleum hydrocarbons in the laboratory. We administered a seawater solution that had been contaminated with total petroleum hydrocarbons to seven treatment groups at different concentrations, while the control group received uncontaminated filtered seawater. The average density of total meiofauna in the experimental microcosms diluted with 0.5%, 1%, 2%, and 4% contaminated seawater was higher than the density in the control. The average density of total meiofauna in the 8%, 15%, and 20% microcosms was lower than the density in the control. The density of nematodes was similar to that of the total meiofauna. Cluster analysis divided the microcosms into group 1 (control, 0.5%, 1%, 2%, and 4% microcosms) and group 2 (8%, 15%, and 20% microcosms). However, SIMPROF analysis showed no significant difference between the two groups (p > 0.05). Bolbolaimus spp. (37.1%) were dominant among the nematodes. Cluster analysis showed similar results for nematode and meiofaunal communities. The total meiofaunal density, nematode density, and number of Bolbolaimus spp. individuals were significantly negatively associated with the concentration of total petroleum hydrocarbons (Spearman correlation coefficients, p < 0.05). Within the nematodes, epistrate feeders (group 2A: 46%) were the most abundant trophic group. Among the treatment groups, the abundance of group 2A increased in low-concentration microcosms and decreased in high-concentration microcosms. Thus, our findings provide information on the effects of oil pollution on meiofauna in the intertidal zones of sandy beaches.     

Long-term variations in macrobenthic community diversity (species number) in the Chokchon macrotidal flat,Incheon, Korea

We performed a time-series analysis of environmental variables and biological parameters to understand temporal variations in the macrobenthic community in the Chokchon macrotidal flats, Incheon, Korea. Bimonthly sampling of macrobenthos and sediments was conducted from May 1989 through November 2001. A decomposition method composed of seasonality, trends and cyclical variation was employed in this study. We focused on trends of the selected variables and assumed that those with positive or negative trends caused changes in biological parameters. Most variables showed strong seasonality (≥ 95% of the total variance). Significant positive trends (p < 0.0001) were observed in sea water temperature and sediment ignition loss. Macrobenthic biomass and species number also showed significant positive trends (p < 0.0001). Biomass indicated that the study area underwent eutrophication since the mid 1990s. A notable increase in species number occurred throughout the study period. The flattened temperature trend after 1994 and intensified eutrophication after the mid 1990s indicated that the species increase in the former period was in response to increasing temperature, while that in the latter period was caused by eutrophication. Although our study suggested an increasing trend in diversity, it is important to check for unexpected changes and establish monitoring programs to provide early warnings, especially in lower latitudes.