The BEAM-project: prediction and assessment of mixture toxicities in the aquatic environment

Freshwater and marine ecosystems are exposed to various multi-component mixtures of pollutants. Nevertheless, most ecotoxicological research and chemicals regulation focus on hazard and exposure assessment of individual substances only, the problem of chemical mixtures in the environment is ignored to a large extent. In contrast, the assessment of combination effects has a long tradition in pharmacology, where mixtures of chemicals are specifically designed to develop new products, e.g. human and veterinary drugs or agricultural and non-agricultural pesticides. In this area, two concepts are frequently used and are thought to describe fundamental relationships between single substance and mixture effects: Independent Action (Response Addition) and Concentration Addition. The question, to what extent these concepts may also be applied in an ecotoxicological and regulatory context may be considered a research topic of major importance, as the concepts would allow to make use of already existing single substance toxicity data for the predictive assessment of mixture toxicities. Two critical knowledge gaps are identified: (a) There is a lack of environmental realism, as a huge part of our current knowledge about the applicability of the concepts is restricted to artificial situations with respect to mixture composition or biological effect assessment. (b) The knowledge on what exactly is needed for using the concepts as tools for the predictive mixture toxicity assessment is insufficient. Both gaps seriously hamper the necessary, scientifically sound consideration of mixture toxicities in a regulatory context.In this paper, the two concepts will be briefly introduced, the necessity of considering the toxicities of chemical mixtures in the environment will be demonstrated and the applicability of Independent Action and Concentration Addition as tools for the prediction and assessment of mixture toxicities will be discussed. An overview of the specific aims and approaches of the BEAM project to fill in the identified knowledge gaps is given and first results are outlined.     

On the circulation in the East Frisian Wadden Sea: numerical modeling and data analysis

 In this paper we use a combination of numerical modeling and data analysis to gain a better understanding of the major characteristics of the circulation in the East Frisian Wadden Sea. In particular, we concentrate on the asymmetry of the tidal wave and its modulation in the coastal area, which results in a complex pattern of responses to the sea-level forcing from the North Sea. The numerical simulations are based on the 3-D primitive equation General Estuarine Transport Model (GETM) with a horizontal resolution of 200 m and terrain-following vertical coordinates. The model is forced at its open boundaries with sea-level data from an operational model for the German Bight (German Hydrographic Office). The validation data for our model simulations include time series of tidal gauge data and surface currents measured at a pile in the back-barrier basin of the Island Langeoog, as well as several ADCP transects in the Accumer Ee tidal inlet. Circulation and turbulence characteristics are investigated for typical situations driven by spring and neap tides, and the analysis is focused on dominating temporal and spatial patterns. By investigating the response of five back-barrier basins with rather different morphologies to external forcing, an attempt is made to elucidate the dominating physical balances controlling the circulation in the individual sub-basins. It is demonstrated that the friction at the seabed tends to slow down the tidal signal in the shallow water. This leads to the establishment of flood dominance in the shallow sea north of the barrier islands. South of the islands, where the water volume of the channels at low tide is smaller than the tidal prism, the asymmetry of the tidal signal is shifted towards ebb dominance, a feature which is particularly pronounced at spring tide. At the northern open boundary, the tidal wave propagating from west to east generates a sea-level difference of ∼1 m along the boundary, and thereby triggers vigorous alongshore currents. The frictional control in the model is located in the inlets, as well as along the northern boundary. The correlation between velocity and turbulent kinetic energy tends to the establishment of a net southward transport, giving theoretical support to the observed accumulation of sediments on the intertidal flats. Weak turbulence along the northern shores of the barrier islands and the small magnitude of the residual currents there promote accumulation of suspended matter in these areas, although wave action will generally counteract this effect. Received: 29 May 2002 / Accepted: 26 September 2002 Responsible Editor: Jean-Marie Beckers Acknowledgements We are indebted to S. Dick for providing the data from the operational model of BSH and to B. Flemming for the useful discussions. The topography data and Fig. 1 have been prepared in cooperation with F. Meyer. Figure 2 has been prepared by G. Brink-Spalink. We also thank for the comments from an anonymous reviewer which helped to improve our paper.     

A novel ozone sensor for direct eddy flux measurements

A small, lightweight (1.5 kg) and fast-response ozone sensor for direct eddy flux measurements has been built. The basis for detection is the chemiluminescence of an organic dye adsorbed on dry silica gel in the reaction with ozone. The chemiluminescence is monitored with a cheap and small blue-sensitive photomultiplier. At a flow rate of 100 l min^-1 the ozone sensor has a 90% response time of significantly better than 0.1 s with a detection limit lower than 50 ppt at S/N=3. There are no interferences from other atmospheric trace gases like NO_x, H₂O₂ and PAN. Water vapour and SO₂ enhance the chemiluminescence efficiency of the ozone sensor. Since their response times are 22 seconds and 30 minutes, respectively, no correlation between rapid ozone fluctuations and those of these two trace gases is noticed by the ozone sensor when operating at a frequency of 10 Hz.The ozone sensor was tested for several weeks in continuous measurements of ozone fluxes and deposition velocities over different croplands using the eddy correlation technique. Good agreement was found between ozone dry deposition velocities derived from profile measurements and by eddy correlation.     

Further studies on the chordal-hop theory of ionospheric long-range propagation

Summary This paper presents new results of the author's investigations on ionospheric propagation in Australia from 1952 to 1957. Considerations on path attenuation are followed by a discussion on the propagation paths to North America and Western Europe. Conclusions are in agreement with the author's previous publications in this field.

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Zusammenfassung In dieser Arbeit behandelt der Verfasser neue Ergebnisse seiner Untersuchungen über ionosphärische Ausbreitung in Australien von 1952 bis 1957. Auf eine Betrachtung über die Signalschwächung folgt eine Diskussion über die Ausbreitungswege nach Nordamerika und Westeuropa. Die Folgerungen stimmen mit den früheren Veröffentlichungen des Verfassers auf diesem Gebiet überein.

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Résumé L'auteur expose les nouveaux résultats de ses recherches sur la propagation ionosphérique en Australie de 1952 à 1957; il considère l'affaiblissement de la propagation des signaux et les chemins suivis par les ondes vers l'Amérique du Nord et vers l'Europe occidentale. Ces résultats concordent avec ceux qu'il a publiés précédemment.

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With 1 Figure     

Spectral Characteristics and Correction of Long-Term Eddy-Covariance Measurements Over Two Mixed Hardwood Forests in Non-Flat Terrain

We present turbulence spectra and cospectra derived from long-term eddy-covariancemeasurements (nearly 40,000 hourly data over three to four years) and the transferfunctions of closed-path infrared gas analyzers over two mixed hardwood forests inthe mid-western U.S.A. The measurement heights ranged from 1.3 to 2.1 times themean tree height, and peak vegetation area index (VAI) was 3.5 to 4.7; the topographyat both sites deviates from ideal flat terrain. The analysis follows the approach ofKaimal et al. (Quart. J. Roy. Meteorol. Soc. 98, 563–589, 1972) whose results were based upon 15 hours of measurements atthree heights in the Kansas experiment over flatter and smoother terrain. Both thespectral and cospectral constants and stability functions for normalizing and collapsingspectra and cospectra in the inertial subrange were found to be different from those ofKaimal et al. In unstable conditions, we found that an appropriate stabilityfunction for the non-dimensional dissipation of turbulent kinetic energy is of the form ₍₎ = (1 - b^-)^-1/4 - c^-, where representsthe non-dimensional stability parameter. In stable conditions, a non-linear functionG_xy() = 1 + b_xy^c _xy (c_xy < 1) was found to benecessary to collapse cospectra in the inertial subrange. The empirical cospectralmodels of Kaimal et al. were modified to fit the somewhat more (neutraland unstable) or less (stable) sharply peaked scalar cospectra observed over forestsusing the appropriate cospectral constants and non-linear stability functions. Theempirical coefficients in the stability functions and in the cospectral models varywith measurement height and seasonal changes in VAI. The seasonal differencesare generally larger at the Morgan Monroe State Forest site (greater peak VAI) andcloser to the canopy.The characteristics of transfer functions of the closed-path infrared gas analysersthrough long-tubes for CO₂ and water vapour fluxes were studied empirically. This was done by fitting the ratio between normalized cospectra of CO₂ or watervapour fluxes and those of sensible heat to the transfer function of a first-order sensor.The characteristic time constant for CO₂ is much smaller than that for water vapour. The time constant for water vapour increases greatly with aging tubes. Three methods were used to estimate the flux attenuations and corrections; from June through August, the attenuations of CO₂ fluxes are about 3–4% during the daytime and 6–10% at night on average. For the daytime latent heat flux (Q_E), the attenuations are foundto vary from less than 10% for newer tubes to over 20% for aged tubes. Correctionsto Q_E led to increases in the ratio (Q_H + Q_E)/(Q^* - Q_G) by about 0.05 to0.19 (Q_H is sensible heat flux, Q^* is net radiation and Q_G is soil heat flux),and thus are expected to have an important impact on the assessment of energy balanceclosure.     

Frequency of Boundary-Layer-Top Fluctuations in Convective and Stable Conditions Using Laser Remote Sensing

The planetary boundary-layer (PBL) height is determined with high temporal and altitude resolution from lidar backscatter profiles. Then, the frequencies of daytime thermal updrafts and downdrafts and of nighttime gravity waves are obtained applying a fast Fourier transform on the temporal fluctuation of the PBL height. The principal frequency components of each spectrum are related to the dominant processes occurring at the daytime and nighttime PBL top. Two groups of cases are selected for the study: one group combines daytime cases, measured in weak horizontal wind conditions and dominated by convection. The cases show higher updraft and downdraft frequencies for the shallow, convective boundary layer and lower frequencies for a deep PBL. For cases characterized by strong horizontal winds, the frequencies directly depend on the wind speed. The temporal variation of the PBL height is determined also in the likely presence of lee waves. For nighttime cases, the main frequency components in the spectra do not show a real correlation with the nocturnal PBL height. Altitude fluctuations of the top of the nocturnal boundary layer are observed even though the boundary layer is statically stable. These oscillations are associated with the wind shear effect and with buoyancy waves at the PBL top.     

Improving a tree-ring reconstruction from west-central Scandinavia: 900?years of warm-season temperatures

Dendroclimatological sampling of Scots pine (Pinus sylvestris L.) has been made in the province of J?mtland, in the west-central Scandinavian mountains, since the 1970s. The tree-ring width (TRW) chronology spans several thousand years and has been used to reconstruct June?CAugust temperatures back to 1632 bc. A maximum latewood density (MXD) dataset, covering the period ad 1107?C1827 (with gap 1292?C1315) was presented in the 1980s by Fritz Schweingruber. Here we combine these historical MXD data with recently collected MXD data covering ad 1292?C2006 into a single reconstruction of April?CSeptember temperatures for the period ad 1107?C2006. Regional curve standardization (RCS) provides more low-frequency variability than ??non-RCS?? and stronger correlation with local seasonal temperatures (51% variance explained). The MXD chronology shows a stronger relationship with temperatures than the TRW data, but the two chronologies show similar multi-decadal variations back to ad 1500. According to the MXD chronology, the period since ad 1930 and around ad 1150?C1200 were the warmest during the last 900?years. Due to large uncertainties in the early part of the combined MXD chronology, it is not possible to conclude which period was the warmest. More sampling of trees growing near the tree-line is needed to further improve the MXD chronology.     

Using paleoclimate proxy-data to select optimal realisations in an ensemble of simulations of the climate of the past millennium

We present and describe in detail the advantages and limitations of a technique that combines in an optimal way model results and proxy-data time series in order to obtain states of the climate system consistent with model physics, reconstruction of past radiative forcing and proxy records. To achieve this goal, we select among an ensemble of simulations covering the last millennium performed with a low-resolution 3-D climate model the ones that minimise a cost function. This cost function measures the misfit between model results and proxy records. In the framework of the tests performed here, an ensemble of 30 to 40 simulations appears sufficient to reach reasonable correlations between model results and reconstructions, in configurations for which a small amount of data is available as well as in data-rich areas. Preliminary applications of the technique show that it can be used to provide reconstructions of past large-scale temperature changes, complementary to the ones obtained by statistical methods. Furthermore, as model results include a representation of atmospheric and oceanic circulations, it can be used to provide insights into some amplification mechanisms responsible for past temperature changes. On the other hand, if the number of proxy records is too low, it could not be used to provide reconstructions of past changes at a regional scale.     

Chemical Mechanism Development: Laboratory Studies and Model Applications

Within the German Tropospheric Research Programme (TFS) numerous kinetic and mechanistic studies on the tropospheric reaction/degradation of the following reactants were carried out: oxygenated VOC, aromatic VOC, biogenic VOC, short-lived intermediates, such as alkoxy and alkylperoxy radicals.At the conception of the projects these selected groups were classes of VOC or intermediates for which the atmospheric oxidation mechanisms were either poorly characterised or totally unknown. The motivation for these studies was the attainment of significant improvements in our understanding of the atmospheric chemical oxidation processes of these compounds, particularly with respect to their involvement in photooxidant formation in the troposphere. In the present paper the types of experimental investigations performed and the results obtained within the various projects are briefly summarised. The major achievements are highlighted and discussed in terms of their contribution to improving our understanding of the chemical processes controlling photosmog formation in the troposphere.