Production of γ-linolenic acid and stearidonic acid by <Emphasis Type="Italic">Synechococcus</Emphasis> sp. PCC7002 containing cyanobacterial fatty acid desaturase genes

Genetic modification is useful for improving the nutritional qualities of cyanobacteria. To increase the total unsaturated fatty acid content, along with the ratio of ω-3/ω-6 fatty acids, genetic engineering can be used to modify fatty acid metabolism. Synechococcus sp. PCC7002, a fast-growing cyanobacterium, does not contain a Δ6 desaturase gene and is therefore unable to synthesize γ-linolenic acid (GLA) and stearidonic acid (SDA), which are important in human health. In this work, we constructed recombinant vectors Syd6D, Syd15D and Syd6Dd15D to express the Δ15 desaturase and Δ6 desaturase genes from Synechocystis PCC6803 in Synechococcus sp. PCC7002, with the aim of expressing polyunsaturated fatty acids. Overexpression of the Δ15 desaturase gene in Synechococcus resulted in 5.4 times greater accumulation of α-linolenic acid compared with the wild-type while Δ6 desaturase gene expression produced both GLA and SDA. Co-expression of the two genes resulted in low-level accumulation of GLA but much larger amounts of SDA, accounting for as much to 11.64% of the total fatty acid content.     

水中荧光计在高混浊水域中的混合扩散实验应用

为了研究长江水域污染物质的输运和扩散规律，作者利用研制成功的水中荧光计在该水域进行了混合扩散实验。该仪器在高混浊水作中对罗丹明B的浓度检测范围为1×10－9～5×10-5g／cm3，精确度≤±10％。本文着重论述使用水中荧光计和荧光示踪染料在流速大、混浊度高的长江九江江段和镇江江段进行水体混合扩散测量实验的情况，结果令人满意，为我国的高混浊水体混合扩散实验研究提供了新的手段和方法。     

利用盐田卤水池塘养殖卤虫的研究

1988年和1989年,作者在山东省盐场利用盐田卤水池进行了卤虫池塘养殖的研究。研究内容包括微藻培养试验、卤虫群体生长能力试验和间捕法养殖卤虫试验。通过试验在6个投饵池塘获得的鲜活卤虫产量为423.5—551.0公斤/亩(平均491.6公斤/亩),在4个不投饵池塘获得的产量为240.5—299.5公斤/亩(平均272.3公斤/亩)。本文报告了以上三个试验的内容和结果,并对卤虫养殖中出现的几个问题进行了讨论。     

Numerical modeling of the elastoplastic damage behavior of dry and saturated concrete targets subjected to rigid projectile penetration

The objective of the present paper is to present a numerical study on the penetration performance of concrete targets with 2 different water contents. Numerical analysis has been performed by using the finite element code Abaqus/Explicit, in which a coupled elastoplastic damage model has been developed for saturated/unsaturated concrete under a wide range of confining pressures. The performance of proposed model has been firstly verified by simulating the triaxial compression tests and penetration tests realized with saturated/dry concretes. Comparisons of available experimental results and numerical simulations show that the proposed model is able to reproduce satisfactorily the mechanical behavior of saturated and dry concretes. A higher failure stress and a more important pores closing are generally obtained in dry concrete samples with respect to saturated ones. Furthermore, the main observed patterns of penetration test realized with saturated concrete targets are also satisfactorily simulated by the numerical results. Therefore, the proposed model is used to numerically predict the penetration performance of dry concrete target, and the penetration performance of dry/saturated concrete target is discussed. We observe that in dry concrete target, the penetration of projectile is strongly declined, and a smaller damage zone is created. The numerical predictions and discussions can help engineers to enhance their understandings on the influence of hydraulic conditions on structural vulnerability of concrete structures subjected to near‐field detonations or impacts.     

国家级太阳辐射测量标准及其质量控制

太阳辐射测量标准仅采取定期参加国际比对的溯源方法不能完全满足质量保证的要求。只有联合国际比对和期间核查的控制方法,才能确保测量标准的测量不确定度持续控制在误差允许范围之内。我国太阳辐射测量标准与世界辐射测量基准 (WRR) 比对结果的不确定度为0.17%,满足世界气象组织 (WMO) 要求,并达到世界先进水平。为了保证在两次国际周期比对间隔之间的准确可靠,并保证其处于良好置信度的校准状态,定期对测量标准的重复性和稳定性进行期间核查, 以图形记忆方式对太阳辐射测量标准的测量过程进行连续和长期的统计控制。通过期间核查,保证了太阳辐射测量标准的重复性不大于0.1%,年稳定性小于0.25%,未超出控制界限且分布呈随机状态,保证了测量过程处于稳定受控状态,满足建标要求。     

地面气象观测站自动检测系统研究

为了解决目前的计量检定标准设备和方法不适于地面气象观测站仪器,检测的自动化水平及工作效率低等矛盾,研究设计了地面气象观测站自动检测系统,特点是将地面气象观测站的温度、湿度、大气压力、风速风向和降水等传感器和地面气象观测站的数据采集器分开进行性能的自动检测.传感器自动检测系统采用多通道设计,可同时进行多个传感器的自动检测并自动输出检测结果.数据采集器自动检测系统可以对气象台站使用的各种不同的地面气象观测站数据采集器系统进行性能测试.文章所设计的地面气象观测站自动检测系统提高了地面自动观测仪器的检测效率,确保了各台站的地面气象自动观测仪器观测数据的准确可靠.     

晴空回波在强对流天气临近预报中的应用

该文介绍了利用多普勒天气雷达资料判断环境风场辐合、辐散的两种方法:径向速度图像特征定性识别法和EVAD技术定量计算法,并结合2009年8月两次强对流天气过程详细介绍了两种方法的具体应用。统计分析了2005-2008年5-9月晴空回波特征以及不同高度散度和对流出现时间的关系,晴空回波出现在距离雷达中心50km范围之内,反射率因子多在10～20dBZ,径向速度大部分为-5～5m/s;强对流天气出现之前,对流层低层均会出现辐合,可以利用低层连续5个体扫出现辐合作为环境场具有辐合条件的指标。根据统计结果建立了强对流天气临近预警系统,该系统在2009年6-8月试运行,预警命中率为88.9%,虚警率为29.8%,临界成功指数为64.5%,辐合提前对流天气出现时间平均为7.1h,对降低强对流天气漏报率,提高强对流天气的临近预报水平有重要意义。     

微斜长石可见光吸收技术在金矿找矿中的应用

含金性不同的徽斜长石在偏振光条件下的可见光吸收光谱，与其不同程度地含有过渡金属离子密切相关，徽斜长石对600－650nm光波的吸收谱线特征，可以用来评价寄主脉的含金性，作为找寻石英钾长石脉型金矿床的重要矿物学标志，为金矿找矿提供了新方法。     

多普勒速度图暴雨判据和短时预报工具研究

对3824-C波段全相参多普勒天气雷达1995-1999年径向速度场资料中逆风区进行统计分析，认为近距离逆风区可成为暴雨短时预报的一个有参考价值的判据，并给出人机交互识别逆风区工具的设计和实现方法。