辽河三角洲地区海平面上升趋势及其影响评估

根据潮位资料分析,辽河三角洲平原和辽东湾东岸近四五十年来相对海平面处于上升趋势,从70年代以来平均每年上升量为5mm左右.考虑到辽河三角洲平原地面下沉以及全球性海平面将加速上升,预计下个世纪内,辽河三角洲平原相对海平面上升的速率将达到8-10mm/a,到2050年相对海平面上升量将达到40～55cm.利用遥感和地理信息系统,对不同的海平面上升量将造成的土地淹没损失进行了预测.如不加防护,相对海平面上升0.5m时,将淹没近4000km2,包括整个营口市区和半个盘锦市区;上升1.0m时,将淹没5000km2.对海平面上升将造成海岸侵蚀、风暴潮和洪涝等灾害加剧等影响也进行了分析.     

褐藻多酚合成的模拟研究

褐藻多酚是间苯三酚的聚合物,结构复杂,有关生物合成途径方面的研究报道甚少。近年发现,褐藻多酚具有多种生物活性和医药价值(Ragan & Glombitza,1986),因此，研究褐藻多酚的人工合成不仅可模似研究褐藻多酚的生物合成过程,而且为寻找替代自然资源打下了基础。 作者在实验中观察到配制的间苯三酚溶液放置一段时间后会逐渐发黄,因此认为间苯三酚在放置过程中发生了氧化聚合,并对此作了进一步研究。     

一种污水排海的近远区计算与控制方法──以大亚湾水容量计算及污水排海为例

针对点容量计算中混合区限制的要求，采用了动量积分法、经验公式法和有限差分法，分别对污水排放后可能引起的近区、过渡区和远区的污水浓度变化进行了模拟预测，并就一定的限制性条件对容量点近区、高浓度混合区及容量点远区影响范围进行了分析。在大亚湾水容量计算及污水排海规划中的应用表明，该法具有计算机时较省、精度相对较高等优点。     

一种共生藻PCR模板DNA的快速制备法

采用Chelex-100树脂法制备了26个物种(分别属于腔肠动物门、海绵动物门和软体动物门)的共生藻以及一株纯培养共生藻的DNA样品。通过PCR扩增叶绿体23S rRNA基因片段获得了26个有效序列,测序比对结果显示获得的序列与扩增目的基因相符。该方法不仅样品消耗量小、操作时间短、设备需求低廉,而且可以避免使用有害化学试剂。这是一种高效、环保的DNA提取方法,可为共生藻分子研究提供技术参考。     

Dynamic Performance Investigation of A Spar-Type Floating Wind Turbine Under Different Sea Conditions

Both numerical calculation and model test are important techniques to study and forecast the dynamic responses of the floating offshore wind turbine (FOWT). However, both the methods have their own limitations at present. In this study, the dynamic responses of a 5 MW OC3 spar-type floating wind turbine designed for a water depth of 200 m are numerically investigated and validated by a 1:50 scaled model test. Moreover, the discrepancies between the numerical calculations and model tests are obtained and discussed. According to the discussions, it is found that the surge and pitch are coupled with the mooring tensions, but the heave is independent of them. Surge and pitch are mainly induced by wave under wind wave conditions. Wind and current will induce the low-frequency average responses, while wave will induce the fluctuation ranges of the responses. In addition, wave will induce the wavefrequency responses but wind and current will restrain the ranges of the responses.     

胶州湾沉积物中石油降解菌的降解能力及多样性研究

2013年青岛输油管道爆炸,大量石油污染了附近海岸。课题组采集了污染的沉积物样品,以原油为唯一碳源和能源,富集了四个石油降解菌群。生物多样性和群落分析表明,Luteibacter、Parvibaculum 和属于食烷菌科的一个属是降解菌群的主要优势菌,都属于变形菌门。从石油降解菌群中分离筛选,获得了9株具有不同16S rRNA基因序列的降解菌,分别属于8个属。重量法测定降解菌的石油降解率,其中5株的石油降解率大于30%。GC-MS分析结果表明,石油降解菌多倾向于降解烷烃,对多环芳烃的降解能力较差,其中5株细菌的烷烃降解率较大,仅1株菌D2对多环芳烃的降解率较大,其降解率在34.9%到77.5%。通过对高通量数据的分析,表明食烷菌属是菌群A和菌群E的主要降解菌群,其中筛选获得的菌株E4可能是菌群E的一株优势降解菌。本研究所筛选菌株证明了其石油降解潜力,为油污染海滩生物修复提供了菌株资源。     

海水温度对衰亡期浒苔释放生源硫影响的模拟研究

为研究浒苔释放生源硫的特征,本文对采集于黄海绿潮中期和末期的浒苔进行了实验室模拟培养,探讨了不同温度对衰亡期浒苔释放生源硫化物的影响。实验结果表明,在10~25℃温度范围内,温度升高能够加速浒苔的衰亡。二甲基硫（DMS）的平均释放速率范围为2.79~150.70 nmol/（L·g·d）,二甲基硫基丙酸内盐（DMSP）的平均释放速率范围为2.16~113.26 nmol/（L·g·d）。温度升高能够使DMS和DMSP的释放速率加快,释放量增加,DMS最大平均释放速率在25℃条件下比10℃条件下升高了约60%,培养液中DMS浓度升高了2~3倍。采集于绿潮末期的浒苔培养液中的DMS和DMSP和采集于绿潮中期的浒苔相比,浓度有所增加,采集于浒苔绿潮末期浒苔培养液中DMS的最高平均浓度为418.41 nmol/L,约为中期的4倍;DMSP的最高平均浓度为316.14 nmol/L,是中期的3倍。浒苔绿潮的爆发会对水体中的硫体系循环产生影响,进而影响该海域生态环境。     

重力活塞取样器取样技术研究

现代海洋沉积物柱状取样技术是一套综合性的高新技术系统,它一般应该包括：优良的取样器具,方便灵活的取样操作设备,高精度的定位导航仪器和高分辨率的地形、地貌、地层探测系统等四个方面。我国和先进国家相比,取样操作设备方面差距最大,应该成为我国今后取样技术研究和发展的重点     

Sulfide and iron control on mercury speciation in anoxic estuarine sediment slurries

In order to understand the role of sulfate and Fe(III) reduction processes in the net production of monomethylmercury (MMHg), we amended anoxic sediment slurries collected from the Venice Lagoon, Italy, with inorganic Hg and either potential electron acceptors or metabolic byproducts of sulfate and Fe(III) reduction processes, gradually changing their concentrations. Addition of sulfide (final concentration: 0.2–6.3 mM) resulted in an exponential decrease in the sulfate reduction rate and MMHg concentration with increasing concentrations of sulfide. Based on this result, we argue that the concentration of dissolved sulfide is a critical factor controlling the sulfate reduction rate, and in turn, the net MMHg production at steady state. Addition of either Fe(II) (added concentration: 0–6.1 mM) or Fe(III) (added concentration: 0–3.5 mM) resulted in similar trends in the MMHg concentration, an increase with low levels of Fe additions and a subsequent decrease with high levels of Fe additions. The limited availability of dissolved Hg, associated with sulfide removal by precipitation of FeS, appears to inhibit the net MMHg production in high levels of Fe additions. There was a noticeable reduction in the net MMHg production in Fe(III)-amended slurries as compared to Fe(II)-amended ones, which could be caused by a decrease in the sulfate reduction rate. This agrees with the results of Hg methylation assays using the enrichment cultures of anaerobic bacteria: whereas the enrichment cultures of sulfate reducers showed significant production of MMHg (4.6% of amended Hg), those of Fe(III), Mn(IV), and nitrate reducers showed no production of MMHg. It appears that enhanced Fe(III)-reduction activities suppress the formation of MMHg in high sulfate estuarine sediments.     

Complete nuclear ribosomal DNA sequence analyses of the black-footed abalone Haliotis iris

The black-footed abalone Haliotis iris is an economically important shellfish species in New Zealand. We successfully amplified, sequenced and analysed the complete nuclear ribosomal DNA (nrDNA) of H. iris. The length of the nrDNA was determined to be around 9.6?kb and included, in order, small subunit ribosomal RNA (nrSSU, 1858bp), internal transcribed spacer (ITS, 749?bp), large subunit ribosomal RNA (nrLSU, 3412bp) and an intergenic spacer (IGS, 3560–3662?bp). The nrLSU genes were identical in two individuals, whereas the nrSSU and ITS regions existed at three and four base differences, respectively. The IGS was more variable than the other nrDNA regions. A phylogenetic tree was constructed based on the ITS sequence datasets, which revealed that Haliotidae has two major subclades, mainly distributed in the North Pacific, Europe and Australia. The complete nrDNA sequence will be useful for the classification, phylogeny and breeding of this shellfish.