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181.
采用随机克隆、功能筛选、逐次排除和同源比较的基因克隆新策略进行克隆假单胞菌(Pseudomonas sp.cn4902)磷酸甘油磷酸酯酶基因的研究。结果表明,该结构基因长819bp,与铜绿假单胞菌的磷酸甘油磷酸酯酶基因的核苷酸一致性达61.5%,氨基酸同源性为56.2%。该基因已输入GenBank数据库,收录号AF348165。将该基因转化大肠杆菌,受体菌在含NaCl 1.0mol/L的培养基中甘油含量升高2.9倍,最终菌浓度提高3.6倍。可见这是一个与生物耐盐性相关的主基因,以其转化、培育耐盐农作物的前景十分光明。  相似文献   
182.
基于“数字海底”系统的理论基础研究和在海底科学应用中的具体实践,提出了“数字海底”的涵义、系统组成和体系结构,对空间数据管理技术、基于XML的WebGIS空间数据表达技术、复杂海底三维建模技术、多源信息的一体化集成显示技术、虚拟现实技术等“数字海底”系统建模中的关键技术进行了探讨,为从事海底资源研究和开发的人员提供各个层次的参考或帮助.  相似文献   
183.
Starting from the widespread phenomena of porous bottoms in the near shore region, considering fully the diversity of bottom topography and wave number variation, and including the effect of evanescent modes, a general linear wave theory for water waves propagating over uneven porous bottoms in the near shore region is established by use of Green‘s scond identity. This theory can be reduced to a number of the most typical mild-slope equations curreutly in use and provide a reliable research basis for follow-up development of nonlinear water wave theory involving porous bottoms.  相似文献   
184.
More and more researches show that neither the critical downward acceleration nor the critical slope of water waves is a universal constant. On the contrary, they vary with particular wave conditions. This fact moders the models either for the probability of wave breaking B or for the whitecap coverage W based on these criteria difficult to apply. In this paper and the one which follows we seek to develop models for the prediction of both B and W based on the kinematical criterion. First, several joint probabihstic distribution functions (PDFs) of wave characteristics are derived, based on which the breaking properties B and W are estimated. The estimation is made on the assumption that a wave breaks ff the horizontal velocity of water particles at its crest exceeds the local wave celerity, and whitecapping occurs in regions of fluid where water particles travel faster than the waves. The consequent B and W depend on wave spectral moments of orders 0 to 4.Then the JONSWAP spectrum is used to represent the fetch-limited sea waves in deep water, so as to relate the probahility of wave breaking and the whitecap coverage with wind parameters. To this end, the time-averaging technique proposed by Glazman (1986) is applied to the estimation of the spectral moments involved, and furthermore, the theoretical models are compared with available observations collected from published literature. From the comparison, the averaging time scale is determined. The final models show that the probability of wave breaking as well as the whitecap coverage depends on the dimensionless fetch. The agreement between these models and the database is reasonable.  相似文献   
185.
An optimization mathematical model of the pile forces for piled breasting dolphins in the open sea under various loading conditions is presented. The optimum layout with the well distributed pile forces and the least number of piles is achieved by the multiplier penalty function method. Several engineering cases have been calculated and compared with the result of the conventional design method. It is shown that the number of piles can be reduced at least by 10%—20% and the piles‘ bearing state is improved greatly.  相似文献   
186.
本文采用动电位极化、线性极化、电偶电流和高压釜挂片失重等法,研究了含氮、硫有机缓蚀剂和/或Me~(2+)对X60钢在CO_2饱和的盐水溶液中的缓蚀作用。结果表明,含氮、硫的有机缓蚀剂在缓蚀过程中,开始是以抑制阳极过程为主,随后是以抑制阴极过程为主的混合型缓蚀剂。如与Me~(2+)复配使用,能明显地提高缓蚀效能。  相似文献   
187.
Semidiurnal and diurnal tidal waves in the Taiwan Strait are computed simultaneously by using finite difference method with two-dimensional nonlinear hydrodynamic equations. The agreement between the computed and observed results is considered satisfactory.  相似文献   
188.
渤海及黄海北部处在中纬度地带,每年冬季都有不同程度的结冰现象。以辽东湾结冰时间最长,全部结冰日数平均为100多天,约占全年的三分之一。从全海区看,建国后冰情较重的有1957、1968、1969、1977年的1、2月份,尤其是1969年,渤海海面发生了该海区历史上罕见的冰封,整个海面几乎全部被海冰所覆盖,严重阻碍了海上的航运交通及海上石油开采工作,从而给国民经济带来很大损失。  相似文献   
189.
High-resolution side-scan mosaics, sediment analyses, and physical process data have revealed that the mixed carbonate/siliciclastic, inner shelf of west-central Florida supports a highly complex field of active sand ridges mantled by a hierarchy of bedforms. The sand ridges, mostly oriented obliquely to the shoreline trend, extend from 2 km to over 25 km offshore. They show many similarities to their well-known counterparts situated along the US Atlantic margin in that both increase in relief with increasing water depth, both are oriented obliquely to the coast, and both respond to modern shelf dynamics. There are significant differences in that the sand ridges on the west-central Florida shelf are smaller in all dimensions, have a relatively high carbonate content, and are separated by exposed rock surfaces. They are also shoreface-detached and are sediment-starved, thus stunting their development. Morphological details are highly distinctive and apparent in side-scan imagery due to the high acoustic contrast. The seafloor is active and not a relict system as indicated by: (1) relatively young AMS 14C dates (<1600 yr BP) from forams in the shallow subsurface (1.6 meters below seafloor), (2) apparent shifts in sharply distinctive grayscale boundaries seen in time-series side-scan mosaics, (3) maintenance of these sharp acoustic boundaries and development of small bedforms in an area of constant and extensive bioturbation, (4) sediment textural asymmetry indicative of selective transport across bedform topography, (5) morphological asymmetry of sand ridges and 2D dunes, and (6) current-meter data indicating that the critical threshold velocity for sediment transport is frequently exceeded. Although larger sand ridges are found along other portions of the west-central Florida inner shelf, these smaller sand ridges are best developed seaward of a major coastal headland, suggesting some genetic relationship. The headland may focus and accelerate the N–S reversing currents. An elevated rock terrace extending from the headland supports these ridges in a shallower water environment than the surrounding shelf, allowing them to be more easily influenced by currents and surface gravity waves. Tidal currents, storm-generated flows, and seasonally developed flows are shore-parallel and oriented obliquely to the NW–SE trending ridges, indicating that they have developed as described by the Huthnance model. Although inner shelf sand ridges have been extensively examined elsewhere, this study is the first to describe them in a low-energy, sediment-starved, dominantly mixed siliciclastic/carbonate sedimentary environment situated on a former limestone platform.  相似文献   
190.
厦门筼筜港的形成与演变   总被引:3,自引:0,他引:3  
本文根据实际调查的资料,概述了筼筜港区的地质地貌特征,重点论述了第四纪沉积物的成因和时代,阐述了该区的形成与演变过程,把它划分为5个发育阶段。  相似文献   
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