Application research of enhanced in-situ micro-ecological remediation of petroleum contaminated soil

Experimental study of enhanced in-situ micro-ecological remediation of petroleum contaminated loess soil was carried out in Zhongyuan oil production areas, and the enhanced in-situ micro-ecological remediation technique includes optimistic in-situ microbial communities, physical chemistry methods, alfalfa planting and regulation of soil environmental elements. Experiments showed that the oil content in the contaminated soil with oil content about 2 898.25 mg/kg can be reduced about 98.61% after in-situ micro-ecological remediation for 99 days, which demonstrated the effectiveness of in-situ micro-ecological remediation methods for petroleum contaminated soil in central plains of China, and explored the practical and feasible application of these methods.     

基于五种油源的中重度风化溢油的常规鉴定指标的应用效果评估

本文利用采集于我国三大油田的五种原油样品开展了长达210天的溢油风化模拟实验,并依据相对偏差和重复性限数学分析法,进行溢油风化过程分析和诊断比值应用效果评估。研究结果表明:经过210天的风化,溢油鉴定诊断比值发生明显改变;其中来源于萜烷、甾烷和多环芳烃的诊断比值变化率要远低于正构烷烃,可用于中长期风化溢油鉴定。此外,研究发现,在这些有效诊断比值中有4个变化率较小,相对偏差低于5%,保持了较好的稳定性,更适合于重度风化溢油鉴定。     

MALBAC技术扩增微生物群落基因组的效率评估

环境样品的低生物量是微生物宏基因组学研究面临的首要挑战,通过基因组扩增技术来满足高通量测序对DNA样品量的需求是最常用的解决策略。MALBAC(Multiple Annealing and Looping Based Amplification Cycles)基因组扩增试剂盒最初为扩增和研究哺乳动物的单细胞基因组而研发。本文中,我们通过人工构建的微生物群落来检测该试剂盒在微生物宏基因组扩增方面的效率和应用可行性。结果表明,每个标准反应中,10 pg的DNA模板量足以满足MALBAC试剂盒对样品扩增的需要。每个标准反应DNA模板用量为10和100 pg时,所扩增DNA样品的基因组覆盖度与原始未扩增样品表现出高度的一致性,证明MALBAC试剂盒扩增效果的高度稳定性和一致性。常用的GenomePlex全基因组扩增试剂盒使我们可以在每个标准反应DNA模板量为100 pg的条件下扩增获得足够的DNA样品,但是结果表明该参照试剂盒无法有效的实现对群落中低丰度细菌菌株基因组的线性扩增。对于MALBAC试剂盒和参照试剂盒而言,在扩增高GC含量的微生物物种基因组DNA方面效率低下。我们的实验结果表明MALBAC试剂盒在高效扩增环境样品宏基因组DNA方面的可行性,但对该试剂盒在扩增环境样品中高GC含量微生物物种方面的适用性存在疑虑。     

一种基于Curvelet变换的合成孔径雷达海洋内波图像的斑点噪声抑制方法

合成孔径雷达是海洋内波研究中最重要的工具之一。雷达图像中的斑点噪声会严重降低图像的质量,这一问题在处理和分析信号较弱的二模态内波信号和上升型内波信号时极为明显。合成孔径雷达图像中的海洋内孤立波的信号具有明显的尺度性和方向性。同时,curvelet变换作为一种同时具备尺度分辨率和方向分辨率的数学变换,能够对一幅雷达图像在不同尺度、不同方向和不同位置上进行分析。本文给出了一个基于curvelet变换的合成孔径雷达海洋内孤立波图像的斑点噪声抑制方法。该方法可简述为：(1)对一幅合成孔径雷达海洋内孤立波图像进行curvelet变换,获得curvelet系数;(2)分别仅仅保留一个尺度的系数,将其它尺度的系数置为零,利用处理之后的系数分别重建图像,得到仅仅用一个尺度的系数重建的图像;(3)分别计算上一步中得到的图像的均方差,根据波浪理论,图像的方差代表能量,方差越大则能量越大,以此可以确定内波信息集中的尺度;(4)在每个尺度下,分别计算每个方向的curvelet系数矩阵的平均值,以此确定内孤立波信号集中的方向;(5)在上两步工作的基础上,仅仅保留内波信号集中的尺度和方向的系数,而将其它尺度和方向的系数置为0,得到一幅提取主波信息的图像;(6)将上一步得到的提取主波信息的图像加回到原始图像中,从而达到增强波浪信息并抑制斑点噪声的目的。大量的实验验证表明,该方法不仅能有效地压制斑点噪声,而且能有效地增强波浪信号。     

Microstructure observations in the upper layer of the South China Sea

A general pattern for turbulent mixing in the upper layer of the South China Sea (SCS) is presented based on TurboMAP measurements in April and May 2010. The turbulence level decreased significantly overall from north to south, and weakened from east to west in the northern SCS. The average dissipation rate north of 18°N reaches 1.69 × 10^?8 W/kg, approximately six times larger than that south of 18°N. The mean mixing efficiency in the SCS is 0.2, with a maximum of 0.31 near the Luzon Strait. At one repeatedly occupied station located in the central deep basin, the dissipation rate varies diurnally in the mixed layer and pycnocline due to diurnal heating and cooling by solar radiation and local barotropic tide, respectively.     

AMSR-E sea surface temperature (SST) charts enhancement

Microwave satellite images used for retrieving sea surface temperatures often have such distortions as noise and blurring of the thermal fronts. An image processing approach based on the Mumford-Shah model of optimal image approximation is considered for the solution to this problem. We divide images into flat areas and frontal zones, and then process these areas separately. Image fragmentation is based on automatic detection of the thermal front lines. SST enhancement in frontal zones is achieved by using image deconvolution methods. It has been shown that SST errors in high gradient areas reach 1–3 °C. The proposed approach can decrease this discrepancy.     

Seasonal variations in the nitrogen isotopic composition of settling particles at station K2 in the western subarctic North Pacific

Intensive observations using hydrographical cruises and moored sediment trap deployments during 2010 and 2012 at station K2 in the North Pacific Western Subarctic Gyre (WSG) revealed seasonal changes in δ ¹⁵N of both suspended and settling particles. Suspended particles (SUS) were collected from depths between the surface and 200 m; settling particles by drifting sediment traps (DST; 100–200 m) and moored sediment traps (MST; 200 and 500 m). All particles showed higher δ ¹⁵N values in winter and lower in summer, contrary to the expected by isotopic fractionation during phytoplankton nitrate consumption. We suggest that these observed isotopic patterns are due to ammonium consumption via light-controlled nitrification, which could induce variations in δ ¹⁵N(SUS) of 0.4–3.1 ‰ in the euphotic zone (EZ). The δ ¹⁵N(SUS) signature was reflected by δ ¹⁵N(DST) despite modifications during biogenic transformation from suspended particles in the EZ. δ ¹⁵N enrichment (average: 3.6 ‰) and the increase in C:N ratio (by 1.6) in settling particles suggests year-round contributions of metabolites from herbivorous zooplankton as well as TEPs produced by diatoms. Accordingly, seasonal δ ¹⁵N(DST) variations of 2.4–7.0 ‰ showed a significant correlation with primary productivity (PP) at K2. By applying the observed δ ¹⁵N(DST) vs. PP regression to δ ¹⁵N(MST) of 1.9–8.0 ‰, we constructed the first annual time-series of PP changes in the WSG. This new approach to estimate productivity can be a powerful tool for further understanding of the biological pump in the WSG, even though its validity needs to be examined carefully.     

Dynamical downscaling of future sea level change in the western North Pacific using ROMS

The future regional sea level (RSL) rise in the western North Pacific is investigated by dynamical downscaling with the Regional Ocean Modeling System (ROMS) with an eddy-permitting resolution based on three global climate models—MIROC-ESM, CSIRO-Mk3.6.0, and GFDL-CM3—under the highest greenhouse-gas emission scenario. The historical run is forced by the air-sea fluxes calculated from Coordinated Ocean Reference Experiment version 2 (COREv2) data. Three future runs—ROMS-MIROC, ROMS-CSIRO, and ROMS-GFDL—are forced with an atmospheric field constructed by adding the difference between the climate model parameters for the twenty-first and twentieth century to fields in the historical run. In all downscaling, the RSL rise along the eastern coast of Japan is generally half or less of the RSL rise maxima off the eastern coast. The projected regional (total) sea level rises along the Honshu coast during 2081–2100 relative to 1981–2000 are 19–25 (98–104), 6–15 (71–80), and 8–14 (80–86) cm in ROMS-MIROC, ROMS-CSIRO, and ROMS-GFDL, respectively. The discrepancies of the RSL rise along the Honshu coast between the climate models and downscaling are less than 10 cm. The RSL changes in the Kuroshio Extension (KE) region in all downscaling simulations are related to the changes of KE (northward shift or intensification) with climate change.