Phytoplankton absorption spectra along the water column in deep North Patagonian Andean lakes (Argentina)

During a summer period we studied the vertical variation of in vivo and chlorophyll a specific phytoplankton absorption spectra in relation to the underwater light climate of ten deep North Patagonian Andean lakes of Argentina. The lakes were thermally stratified, and the underwater light climate was characterized by extended euphotic zones which included highly illuminated epilimnetic layers (both UVR and PAR) and metalimnia exposed to dim blue-green light. Most of the lakes presented the development of Deep Chlorophyll Maxima (DCM) at the metalimnetic layers, near 1% of surface PAR irradiance. Analyzing the fourth-derivative plots of in vivo phytoplankton absorption spectra [d^IVa_ph(λ)], we were able to identify several maxima absorption values attributed to different pigments. Considering lakes with DCM, a significant positive linear relationship was found between d^IVa_ph (495–500 nm) normalized by chlorophyll a and downward irradiance. Indeed, a negative significant relationship was found between d^IVa_ph (495–500 nm) normalized by chlorophyll a and diffuse PAR attenuation coefficients. These results point out an increase in the relative concentration of different carotenoids at surface layers indicating the role of photoprotection of these pigments. On the other hand, significant negative linear relationships were found between fourth-derivative spectra normalized by chlorophyll a at 650, 590–595, 560–565 and 520–525 nm and downward irradiance. These results indicated an increase in the relative concentration of photosynthetic accessory pigments at deep layers of the euphotic zone. Furthermore, we found a decrease in depth of specific absorption spectra at 440, 670 nm and in the ratio a_ph* (440 nm) to a_ph* (670 nm). This pattern was associated with the package effect concept. The increase in relative photosynthetic accessory pigment concentrations and the decrease in values of specific absorption spectra at the bottom of the euphotic zone were attributed to changes in phytoplankton communities between surface and deep layers. These outcomes pointed out that the underwater light climate and temperature water structure are, like in marine systems, very important factors governing the distribution of phytoplanktonic organisms. In addition, the possession of specific photosynthetic accessory pigments suggests that dominant species in the DCM are well adapted to these dim blue-green light scenarios.     

Role of shellfish hatchery as a reservoir of antimicrobial resistant bacteria

The main aim of this study was to determine the occurrence of resistant bacteria in florfenicol-treated and untreated scallop larval cultures from a commercial hatchery and to characterize some selected florfenicol-resistant strains. Larval cultures from untreated and treated rearing tanks exhibited percentages of copiotrophic bacteria resistant to florfenicol ranging from 0.03% to 10.67% and 0.49–18.34%, respectively, whereas florfenicol resistance among oligotrophic bacteria varied from 1.44% to 35.50% and 3.62–95.71%, from untreated and treated larvae, respectively. Florfenicol resistant microbiota from reared scallop larvae mainly belonged to the Pseudomonas and Pseudoalteromonas genus and were mainly resistant to florfenicol, chloramphenicol, streptomycin and co-trimoxazole. This is the first study reporting antimicrobial resistant bacteria associated to a shellfish hatchery and the results suggest that a continuous surveillance of antimicrobial resistance even in absence of antibacterial therapy is urgently required to evaluate potential undesirable consequences on the surrounding environments.     

<Emphasis Type="Italic">C</Emphasis>/<Emphasis Type="Italic">N</Emphasis><Subscript>0</Subscript> estimators for high-sensitivity snapshot GNSS receivers

We address the problem of estimating the carrier-to-noise ratio (C/N₀) in weak signal conditions. There are several environments, such as forested areas, indoor buildings and urban canyons, where high-sensitivity global navigation satellite system (HS-GNSS) receivers are expected to work under these reception conditions. The acquisition of weak signals from the satellites requires the use of post-detection integration (PDI) techniques to accumulate enough energy to detect them. However, due to the attenuation suffered by these signals, estimating their C/N₀ becomes a challenge. Measurements of C/N₀ are important in many applications of HS-GNSS receivers such as the determination of a detection threshold or the mitigation of near-far problems. For this reason, different techniques have been proposed in the literature to estimate the C/N₀, but they only work properly in the high C/N₀ region where the coherent integration is enough to acquire the satellites. We derive four C/N₀ estimators that are specially designed for HS-GNSS snapshot receivers and only use the output of a PDI technique to perform the estimation. We consider four PDI techniques, namely non-coherent PDI, non-quadratic non-coherent PDI, differential PDI and truncated generalized PDI and we obtain the corresponding C/N₀ estimator for each of them. Our performance analysis shows a significant advantage of the proposed estimators with respect to other C/N₀ estimators available in the literature in terms of estimation accuracy and computational resources.     

Copper accumulation by bacteria and transfer to scallop larvae

A bacterial copper-resistant strain was isolated from a hatchery-conditioned adult of the scallop Argopecten purpuratus and was identified as Vibrio sp. according to its physiological characteristics. The lowest concentration of Cu2+ required for its complete inhibition in VNSS medium was 50 microg ml(-1). The Vibrio strain was found to accumulate copper, exhibiting cellular and loosely bound copper levels of 201.14 and 493.21 microg g(-1) dry weight, respectively, after 24 h of incubation in VNSS medium supplemented with 15 microg ml(-1) of Cu2+, with cellular concentration factors of 10.17 and 14.35 after 12 and 24 h of exposure. When a scallop larvae culture was exposed to a concentration of 6.46 x 10(2) CFU ml(-1) of Cu-enriched Vibrio, they accumulated 20.42 +/- 1.12 and 30.96 +/- 1.85 microg Cu2+ g(-1) dry weight after 12 and 24 h, respectively. This study suggests that bacterial copper accumulation could be very active in marine environments increasing the occurrence of copper transfer to marine food chains.     

Revised tectonic implications for the magnetic anomalies of the western Weddell Sea

Ten years after the USAC (U.S.–Argentina–Chile) Project, which was the most comprehensive aeromagnetic effort in the Antarctic Peninsula and surrounding ocean basins, questions remain regarding the kinematics of the early opening history of the Weddell Sea. Key elements in this complex issue are a better resolution of the magnetic sequence in the western part of the Weddell Sea and merging the USAC data set with the other magnetic data sets in the region. For this purpose we reprocessed the USAC data set using a continuation between arbitrary surfaces and equivalent magnetic sources. The equivalent sources are located at a smooth crustal surface derived from the existing bathymetry/topography and depths estimated by magnetic inversions. The most critical area processed was the transition between the high altitude survey over the Antarctic Peninsula and the low altitude survey over the Weddell Sea that required downward continuation to equalize the distance to the magnetic source. This procedure was performed with eigenvalue analysis to stabilize the equivalent magnetic source inversion.The enhancement of the Mesozoic sequence permits refining the interpretation of the seafloor-spreading anomalies. In particular, the change in shape and wavelength of an elongated positive in the central Weddell Sea suggests that it was formed during the Cretaceous Normal Polarity Interval. The older lineations in the southwestern Weddell Sea are tentatively attributed to susceptibility contrasts modeled as fracture zones. Numerical experimentation to adjust synthetic isochrons to seafloor-spreading lineations and flow lines to fracture zones yields stage poles for the opening of the Weddell Sea since 160 Ma to anomaly 34 time. The corresponding reconstructions look reasonable within the known constraints for the motions of the Antarctic and South America plates. However, closure is not attained between 160 and 118 Ma if independent published East Antarctica–Africa–South America rotations are considered. The lack of closure may be overcome by considering relative motion between the Antarctic Peninsula and East Antarctica until 118 Ma time, an important component of convergence.     

A new jadeitite jade locality (Sierra del Convento, Cuba): first report and some petrological and archeological implications

A new jadeitite jade locality has been discovered in the serpentinite-matrix subduction mélange of the Sierra del Convento (eastern Cuba) in a context associated with tectonic blocks of garnet-epidote amphibolite, tonalitic–trondhjemitic epidote gneiss, and blueschist. The mineral assemblages of jadeitite jade and jadeite rocks are varied and include combinations of jadeite, omphacite, albite, paragonite, analcime, clinozoisite-epidote, apatite, phlogopite, phengite, chlorite, glaucophane, titanite, rutile, zircon, and quartz formed during various stages in their P–T evolution. Field relationships are obscure, but some samples made almost exclusively of jadeite show evidence of crystallization from fluid in veins. In one of these samples studied in detail jadeite shows complex textural and chemical characteristics (including oscillatory zoning) that denote growth in a changing chemical medium. It is proposed that interaction of an Al–Na rich fluid with ultramafic rocks produced Al–Na–Mg–Ca fluids of varying composition. Episodic infiltration of these fluids, as a result of episodic opening of the veins, developed oscillatory zoning by direct precipitation from fluid and after reaction of fluid with pre-existing jadeite. The latest infiltrating fluids were richer in Mg–Ca, favouring the formation of omphacite and Mg–Ca rich jadeite in open voids and the replacement of earlier jadeite by fine-grained omphacite + jadeite at 550–560°C. This new occurrence of jadeite in Cuba opens important perspectives for archeological studies of pre-Columbian jade artifacts in the Caribbean region.     

Petroleum systems modeling in tectonically complex areas — A 2D migration study from the Neuquen Basin, Argentina

A roughly 30 km 2D section across the Faja Plegada y Corrida del Agrio in the Neuquen Basin, Argentina, is the basis for a petroleum systems study, including structural restoration through time, deposition and erosion, pressure, temperature and migration modeling. Integrating tectonic and petroleum systems modeling proved critical to properly evaluate sub-thrust plays. Multi-dimensional modeling of dynamic processes allows accurate assessment and prediction of the potential of petroleum systems in tectonically complex areas such as the Neuquen Basin.     

The Aguacaliente Fault,source of the Cartago 1910 destructive earthquake (Costa Rica)

On 4 May 1910, the most destructive earthquake in the history of Costa Rica (Ms 6.4) destroyed the city of Cartago, a major city located in the Valle Central of Costa Rica. Using both palaeo‐seismological and morphotectonic analyses, we have found evidence that points to the Aguacaliente Fault (AF) as the source of this earthquake. This structure is a N100° E trending, strike‐slip fault situated to the south of Cartago and within a wide band of deformation. We excavated two trenches near Bermejo, south of Cartago. We found evidence of three surface ruptures within the last 1000 years on this fault. The age of the most recent rupture is consistent with the Cartago 1910 earthquake. The AF is a seismogenic source capable of producing large earthquakes (Mw 6.5–6.9) with an estimated recurrence interval of about 500 years.     

Distribution of major and trace elements in La Luna Formation, Southwestern Venezuelan Basin

The La Luna Formation (Maraca section), Maracaibo Basin, was studied by means of V and Ni analysis of the bitumen, total organic carbon (TOC), total sulfur (St), major elements (Si, Al, Fe, Mg, Mn, Ca, Ti, Na, K, P), trace elements (V, Ni, Co, Cr, Cu, and Zn), and electron microprobe analysis (EPMA) of the whole rock, and St, major elements (Si, Al, Fe, Mg, Mn, Ca, Ti, Na, K, P), trace elements (V, Ni, Co, Cr, Cu, Zn, Mo, Ba, U, Th) and rare earth elements (La, Ce, Nd, Sn, Eu, Th, Yb, Lu) of the carbonate-free fraction. The results are discussed based on the organic and inorganic association of trace elements and their use as paleoenvironmental indicators of sedimentation. An association between V and organic matter is suggested by means of correlation between V and Ni vs. TOC, the use of EPMA (whole rock) and V and Ni concentrations (carbonate-free fraction), whereas Ni is found in the organic matter and the sulfide phase. Fe is present as massive and framboidal pyrite, whereas Zn precipitates into a separate phase (sphalerite), and Ni, Cu and, in some cases, Zn, can be found as sulfides associated with pyrite. Concentrations of V and Ni (bitumen), TOC, St, V, Ni, Cr, Cu and Zn (whole rock), U, Th, Mo (carbonate-free fraction) are indicative of changes in the dysoxic sedimentation conditions in the chert layers (TOC, St, V, Ni, Cu and low Zn and V/Cr <4) to euxinic anoxic conditions in the argillaceous limestone (TOC, St, V, Ni, Cu and high Zn and V/Cr >4). In the sequence corresponding to the argillaceous limestone, variations in the concentrations of TOC, St, V, Ni, Zn, Cu and Cr (whole rock) can be observed, also suggesting variable sedimentation conditions. The following is proposed: (i) sedimentation intervals under euxinic conditions associated with high contribution and/or preservation of organic matter, allowing a high concentration level of V and Ni in the organic phase and the accumulation of Cu, Zn and Ni (in a smaller proportion) in the sulfide phase; (ii) sedimentation intervals under anoxic conditions and in the presence of relatively lower H₂S, which allowed lower concentrations of V and Ni in the organic phase and higher concentrations of Cu, Zn, and Ni in the sulfide phase. Rare earth elements (REE) concentrations exhibit a marked increase in Ce, Nd, Sm, Eu, Y and Lu for the QM-3 interval, relative to Post-Archean Average Shale (PAAS). REE enrichment in shales has been related to the presence of phosphate minerals such as monazite or apatite. However, these minerals were not detected through XRD or EPMA in the whole rock or in the carbonate-free fraction. The association of REE with organic matter is suggested due to the absence of phosphate minerals, although assessment of these elements require further analysis.     

Holocene vegetation and climate change recorded in alpine bog sediments from the Borreguiles de la Virgen,Sierra Nevada,southern Spain

High-resolution pollen and magnetic susceptibility (MS) analyses have been carried out on a sediment core taken from a high-elevation alpine bog area located in Sierra Nevada, southern Spain. The earliest part of the record, from 8200 to about 7000 cal yr BP, is characterized by the highest abundance of arboreal pollen and Pediastrum, indicating the warmest and wettest conditions in the area at that time. The pollen record shows a progressive aridification since 7000 cal yr BP that occurred in two steps, first shown by a decrease in Pinus, replaced by Poaceae from 7000 to 4600 cal yr BP and then by Cyperaceae, Artemisia and Amaranthaceae from 4600 to 1200 cal yr BP. Pediastrum also decreased progressively and totally disappeared at ca. 3000 yr ago. The progressive aridification is punctuated by periodically enhanced drought at ca. 6500, 5200 and 4000 cal yr BP that coincide in timing and duration with well-known dry events in the Mediterranean and other areas. Since 1200 cal yr BP, several changes are observed in the vegetation that probably indicate the high-impact of humans in the Sierra Nevada, with pasturing leading to nutrient enrichment and eutrophication of the bog, Pinus reforestation and Olea cultivation at lower elevations.