Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels

Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp aquaculture.The effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp(L.vannamei) were investigated.This involved an examination of growth performance,glutamate dehydrogenase(GDH) and Na+-K+ ATPase mRNA expression,,and GDH activity in muscles and gills.Three experimental diets were formulated,containing 25%,40%,and 50% dietary protein,and fed to the shrimp at a salinity of 25.After 20 days,no significant difference was observed in weight gain,though GDH and Na+-K+ ATPase gene expression and GDH activity increased with higher dietary protein levels.Subsequently,shrimp fed diets with 25% and 50% dietary protein were transferred into tanks with salinities of 38 and 5,respectively,and sampled at weeks 1 and 2.Shrimp fed with 40% protein at 25 in salinity(optimal conditions) were used as a control.Regardless of the salinities,shrimp fed with 50% dietary protein had significantly higher growth performance than other diets;no significant differences were found in comparison with the control.Shrimp fed with 25% dietary protein and maintained at salinities of 38 and 5 had significantly lower weight gain values after 2 weeks.Ambient salinity change also stimulated the hepatosomatic index,which increased in the first week and then recovered to a relatively normal level,as in the control,after 2 weeks.These findings indicate that in white shrimp,the specific protein nutrient and energy demands related to ambient salinity change are associated with protein metabolism.Increased dietary protein level could improve the osmoregulation capacity of L.vannamei with more energy resources allocated to GDH activity and expression.     

Early diagenesis in rapidly accumulating sediments on the Alboran slope, ODP site 976

The oxidation and reduction that occur during early diagenesis of sediments has been studied in the interstitial waters of a rapidly accumulating sedimentary sequence from the Mediterranean margin of Spain. A series of reactions that are mediated by progressively lower free energy derived from oxidation of organic matter is evident in the sedimentary sequence. Iron and manganese are rapidly reduced. Phosphate and alkalinity maxima at a subbottom depth of 15?m indicate maximal organic matter degradation. Methane first appears at ～20?m subbottom after sulfate is depleted, and its concentrations quickly climb.     

Deriving the coronal hole electron temperature: electron density dependent ionization / recombination considerations

Comparison of appropriate theoretically derived line ratios with observational data can yield estimates of a plasma’s physical parameters,such as electron density or temperature.The usual practice in the calculation of the line ratio is the assumption of excitation by electrons/protons followed by radiative decay.Furthermore,it is normal to use the so-called coronal approximation,i.e.one only considers ionization and recombination to and from the ground-state.A more accurate treatment is to include ionizati...     

ΛCDM predictions for galaxy protoclusters – I. The relation between galaxies, protoclusters and quasars at z∼ 6

Motivated by recent observational studies of the environment of   z ∼ 6  QSOs, we have used the Millennium Run (MR) simulations to construct a very large  (∼4°× 4°)  mock redshift survey of star-forming galaxies at   z ∼ 6  . We use this simulated survey to study the relation between density enhancements in the distribution of i ₇₇₅-dropouts and Lyα emitters, and their relation to the most massive haloes and protocluster regions at   z ∼ 6  . Our simulation predicts significant variations in surface density across the sky with some voids and filaments extending over scales of 1°, much larger than probed by current surveys. Approximately one-third of all   z ∼ 6  haloes hosting i -dropouts brighter than   z = 26.5  mag  (≈ M *_{UV, z =6})  become part of   z = 0  galaxy clusters. i -dropouts associated with protocluster regions are found in regions where the surface density is enhanced on scales ranging from a few to several tens of arcminutes on the sky. We analyse two structures of i -dropouts and Lyα emitters observed with the Subaru Telescope and show that these structures must be the seeds of massive clusters in formation. In striking contrast, six   z ∼ 6  QSO fields observed with Hubble Space Telescope show no significant enhancements in their i ₇₇₅-dropout number counts. With the present data, we cannot rule out the QSOs being hosted by the most massive haloes. However, neither can we confirm this widely used assumption. We conclude by giving detailed recommendations for the interpretation and planning of observations by current and future ground- and space-based instruments that will shed new light on questions related to the large-scale structure at   z ∼ 6  .     

Episodic mesozoic volcanism in Namibia and Brazil: A K—Ar Isochron study bearing on the opening of the south atlantic

An attempt is made to find a more objective and precise basis for the correlation of volcanics from southwestern Africa and South America than is possible by frequency diagrams of individual K—Ar ages. This leads to a critical appraisal of conventionally calculated K—Ar ages with the conclusion thata priori assumption regarding the isotopic composition of non-radiogenic argon and, hence, the standard atmospheric correction, are no longer tenable.K—Ar isotoopic data on Mesozoic basalts and dolerites from Namibia and Brazil are presented in terms of an isochron model. Plots for cogenetic rocks are unacceptably scattered on a “radiogenic”⁴⁰Ar vs. K diagram, but show a high degree of collinearity on⁴⁰Ar/³⁶Ar diagrams0K/³⁶Ar diagrams. Using the latter plots, a number of isochrons are generated which indicate that Mesozoic volcanism in these regions occured as several discrete episodes of fairly short duration. Effusion of the extensive Serra Geral basalts of Brazil and the Kaoko basalts of Namibia is shown to have occured simultaneeously at 121 m.y.B.P. Basalts from a series of boreholes along the central Parana Basin, as well as a group of dykes from Sao Paulo, yield isochrons of 128 m.y., which coincides with the postulated onset of separation of Africa and South America based on marine magnetic anomalies. Linear dyke swarms along the Namibian seaboard, interpreted as an expression of the earliest rift phase, have an isochron age of 134 m.y. Sills and dykes, mainly from southern Namibia, with isochron ages of 183 m.y. are considered to be the westernmost manifestation of Stormberg volcanism, not necessarily related to rifting. Most of the igneous suites examined have initial⁴⁰Ar/³⁶Ar ratios significantly different from the modern atmospheric value.     

The effects of magnetic structure on the conduction cooling of flare loops

A model of the sheared magnetic field in a coronal loop is used to evaluate the average cross-field suppression of axial thermal conduction. If the energy source is uniform in radius, this can lead to heat-flux reduction by a factor greater than three. When the source is annular, in a region of radius where the current density and shear are peaked, the effect can be significantly larger. In one extreme case, however, in which magnetic tearing provides the heating in a very narrow layer, the spatial resonance of the source excitation in a long loop leads to approximately axial conduction.     

OGO-4 observations of the ultraviolet auroral spectrum

Auroral ultraviolet spectra in the range 1200–3200 A have been obtained by the spectrometer onboard the OGO-4 satellite. Emissions of N₂, H, O and N are readily identified. Atomic and molecular intensities are deduced from the comparison with a synthetic spectrum and compare reasonably well with some previous measurements and calculations. A feature at 2150 A is assigned to the (1-0) NO γ band. Taking into consideration the various excitation mechanisms of NO(A²σ) we propose that the energy transfer from N₂ metastable molecules to oxygen accounts for the excitation of the NO γ bands. In particular, we suggest that the resonant reaction between O₂ and highly metastable N₂(W³Δ) molecules may be a major source of NO(A²σ).