Using unmanned underwater vehicles as research platforms in coastal ocean studies

The advantages of using unmanned underwater vehicles in coastal ocean studies are emphasized. Two types of representative vehicles, remotely operated vehicle (ROV) and autonomous underwater vehicle (AUV) from University of South Florida, are discussed. Two individual modular sensor packages designed and tested for these platforms and field measurement results are also presented. The bottom classification and albedo package, BCAP, provides fast and accurate estimates of bottom albedos, along with other parameters such as in-water remote sensing reflectance. The real-time ocean bottom optical topographer, ROBOT, reveals high-resolution 3-dimentional bottom topography for target identification. Field data and results from recent Coastal Benthic Optical Properties field campaign, 1999 and 2000, are presented. Advantages and limitations of these vehicles and applications of modular sensor packages are compared and discussed.     

Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.     

场认知方式、性别和惯用空间语对地理空间定向能力影响的实验研究

目前人们对空间定向能力影响因素的研究多关注性别与空间定向能力的关系,较少涉及场认知方式、惯用空间语等因素对空间定向能力的影响。本文以空间认知眼动实验的方式展开研究,通过国内版镶嵌图形测验（EFT）、问卷调查等途径获取被试的场认知方式、性别和惯用空间语等基本信息,使其在控制单一环境变量的虚拟三维空间中完成不同难度的定向任务。进而构建正确率和效率2个指标来衡量空间定向能力,通过统计分析确定空间定向能力的影响因素。研究结果表明：（1）不同场认知方式和不同惯用空间语人群的定向正确率和效率差异统计结果均不显著,场认知方式和惯用空间语对空间定向能力无显著影响;（2）不同性别人群之间定向效率无显著差异而正确率存在显著差异,性别对空间定向能力具有显著影响,男性的空间定向能力较女性更强。     

Supplementation of Yeast Extract to Practical Diet Improves the Growth,Anti-Oxidative Capacity and Intestinal Morphology of Shrimp Litopenaeus vannamei

Two control diets based on the commercial formula were designed to contain high(27%, D1) and low(22%, D2) levels of fish meal, respectively. Into D2, 500, 1000 and 1500 mg kg~(-1) of yeast extract were added, respectively, yielding three experimental diets(YE1 through YE3). Shrimp(initial body weight 0.30 g ± 0.02 g) were fed with the experimental diets, five tanks each diet and 30 shrimp individuals each tank, for 8 weeks, and then challenged with Vibrio parahaemolyticus. The results showed that the specific growth rate(SGR) of shrimp in D2 was significantly lower than that of shrimp in D1(P 0.05). The SGR of shrimp in YE3 was similar to that of shrimp in D1. The feed intake of shrimp was similar between D1 and D2. The feed conversion ratio and protein efficiency ratio of shrimp were similar among all diets(P 0.05). YE significantly improved the activity of glutathione S-transferase. The concentration of glutathione(GSH) and the total serum anti-oxidative capacity(T-AOC) of D1 were significantly higher than those of shrimp feeding other diets(P 0.05). The content of serum malondialdehyde of shrimp feeding YE2 and YE3 was significantly lower than that of shrimp feeding D2(P 0.05). The thickness of intestine muscular layer of shrimp feeding YE1 and YE2 was similar to that of shrimp feeding D1. The shrimp feeding YE1 showed the highest villus height of intestine among all groups. The cumulative mortality after challenging was similar among all groups(70.00%–86.67%)(P 0.05). In conclusion, 1000–1500 mg kg~(-1) of YE was suggested to be supplemented into the practical diets to improve the growth, anti-oxidative capacity and intestinal morphology of shrimp L. vannamei.     

2017-08-09新疆精河Ms6.6地震附近区域相对应变的变化分析

2017年精河M_S6.6地震震前,尼勒克、巴伦台和小泉沟台钻孔应变仪记录到显著的应变变化,经现场落实确认,异常是可靠的。根据这3个台的记录资料计算相对应变的变化,结果表明,地震前最大（小）主应变大小和方向分别出现明显加速和急剧偏转变化。此外还发现,相对应变场异常期间主方向与震源机制解P轴方位较为一致。     

2000-2014年中国重特大煤矿事故时空分布分形特征

本文汇总整理了2000-2014年中国发生的472起重特大煤矿事故,获得其空间分布状况;基于分形理论,利用网格法统计不同网格尺度下覆盖重特大煤矿事故的非空网格中的事故数量,计算煤矿事故的全国、三大自然地理区以及干湿分区的分维值,分析其空间分布分形特征;利用R/S方法研究15年来重特大煤矿事故发生的时间分形特征。研究表明,从全国范围来看,重特大煤矿事故具有较好的空间分形特性,但不同区域的重特大煤矿事故的分形特性却具有很大差别。15年来发生重特大事故数量及事故死亡人数逐年下降,由R/S分析得到的Hurst指数表明事故时间序列的分形特征客观存在,并且年发生事故数量及事故死亡人数量减少趋势。     

2016年门源Ms6.4地震前祁连山周边断层活动分析

基于青藏高原东北缘地区最新的跨断层资料,利用时序曲线分析、断层速率合成分析和垂直形变累积率分析等多种方法进行计算,从不同角度和范围提取2016年门源Ms6.4地震震前异常特征,揭示断层活动。结果显示：1）震中距150 km以内多个场地出现了典型的以加速张性变化为主的短期异常,150~300 km内的异常以趋势变化为主;2）祁连山-海原断裂带上异常场地的空间分布表现出由祁连山断裂带中部集中向东迁移至中东部集中的特征,海原断裂上异常有所增多,此类现象与2003年民乐6.1级地震前的现象极其相似;3）祁连山断裂自2014年以来合成速率偏离正常动态值较远,容易积累应变。垂直形变趋势累积率的计算结果显示,地震震中位于形变积累的高值区。     

Spermatozoid life-span of two brown seaweeds, Saccharina japonica and Undaria pinnatifida, as measured by fertilization efficiency

During sexual reproduction of seaweeds, spermatozoid (sperm) discharge is triggered by chemical messengers (pheromones) released by the female gametes. The chemotactic ability of the sperm ensures fertilization success. Using unialgal male and female gametophyte material under designated standard gametogenesis testing (SGT) conditions, the potential life-span of the sperm of two seaweeds, Saccharina japonica and Undaria pinnatifida, was assessed by their ability to fertilize eggs. Results show that within 20-30 min after being discharged, sperm of both species could complete fertilization without an apparent decline in fertilization rate. Although fertilization rate 60–120 min after sperm discharge dropped significantly in both species, some sperm were viable enough to fertilize the eggs. In S. japonica, at 12°C, some sperm were able to fertilize eggs up to 12 h after discharge. In both species, egg discharge rates (EDR) in the male and female mixed positive controls were significantly higher than those of all the sperm-testing groups. Doubling the seeded male gametophytes of S. japonica in the SGT tests significantly increased the EDR, further confirming the effect of the presence of the male on the female in terms of facilitating egg discharge from oogonia.     

莱芜市农村土地确权登记问题与分析

莱芜市在全省率先启动了农村宅基地总登记,探索集体建设用地使用权抵押登记,在当前农村宅基地登记发证中存在着违法用地、产权不清晰、一户多宅、跨区域购房等问题,与宅基地使用权有效行使不相适应。因此,需加强宅基地的管理和登记的政策研究,制定可行的政策与措施,其中尤其要加强对"一户多宅"问题的处理。     

BDS区域卫星导航系统空间信号精度评估

针对BDS区域卫星导航系统的空间信号精度评估问题,分析利用精密星历进行空间信号评估的算法以及SISURE的投影原理,讨论BDS不同星座的参数选择。估计BDS各个卫星的空间信号精度,估算出SISURE综合误差的平均值为1.54 m,与GPS的空间信号精度还存在一定差距。针对个别导航卫星某些分量误差偏大的情况进行分析,发现GEO卫星SISURE个别时段较差和轨道机动有一定关系,并明确了BDS实际的空间信号精度,可为BDS用户提供一定参考。