Application of quantitative PCR method in detection of Lymphocystis disease virus China （LCDV-cn） in Japanese flounder （Paralichthys olivaceus）

Lymphocystis disease causes serious economic losses in the fish farming industry. The causative agent of the disease is Lymphocystis disease virus China (LCDV-cn), which has a wide range of hosts. Based on competitive quantitative PCR technology, we established a method to quantify the LCDV-cn in tissue. Results demonstrate that the average amount of LCDV-cn in the peripheral blood of infected flounder with evident tumors is about 106virions/ml while the average amount in those flounder with no evident tumor but cultured with the flounder with evident tumor is about 104virions/ml. No virus was found in the negative samples of flounder.     

Transformation of Carbon and Nitrogen by Earthworms in the Decomposition Processes of Broad-leaved Litters

Earthworms are the important constituents in the decayed food web and the main ecological conditioners in the process of decomposition and nutrient mineralization. The transformation of organic carbon (C) and total nitrogen (N) in the broad-leaved litters ingested by earthworms was researched by means of a laboratory experiment. Experimental samples were collected from broad-leaved Korea Pine mixed forest in Liangshui National Natural Reserve (47°10′50″N, 128°53′20″E) in the northeastern Xiao Hinggan Mountains of Northeast China. The contents of organic C and total N in earthworms, leaf litters and earthworm faeces were analyzed. Results show that the organic C content was in the fol- lowing order: leaf litters>faeces>earthworms, while total N content was contrary to that of the organic C. The organic C contents in the different leaf litters were in the following order: Tilia amurensis>Betula costata>Acer mono, whereas the total N contents in the different leaf litters were: Betula costata>Tilia amurensis>Acer mono. The contents of organic C and total N in the faeces from the different leaf litters were almost consistent with the contents of the leaf litters. After the leaf litters were ingested by earthworms, the organic C, which was transformed to increase earthworms' weights, ac- counted for 3.90%-13.31% of the total ingestion by earthworms, while that in the earthworm faeces accounted for 6.14%-13.70%. The transformed organic C through the other metabolism (e.g., respiration) of earthworms accounted for 75.04%-89.92%. The ingested organic C by earthworms was mostly used for metabolic activities. The N ingested by earthworms was less than organic C. It is estimated that 37.08% of total N was transformed to increase the earthworm's weight, 19.97% into earthworm faeces and 47.86% for the consumption of the earthworm's activities. The earthworms not only increased the content of organic C and total N in the soil, but also decreased the values of C/N in the soil and leaf litters. Earthworms play a major role in the leaf litters' decomposition and transformation.     

EVIDENCE FOR ABRUPT CLIMATIC CHANGES ONNORTHWESTERN MARGIN OF EAST ASIAN MONSOON REGION DURING LAST DEGLACIATION

Based on investigations of the Zhongwei Nanshan aeolian section situated in the southeastern margin of Tengger Dcsert, carbon-14 and TL (thermoluminescence) dating results and paleoclimatic proxies such as magnetic susceptibility and grain size, we inferred that the northwestern margin of East Asian monsoon region experienced abrupt climatic changes during the last deglaciation. Six oscillation events were identified: Oldest Dryas, Belling, Older Dryas, AllerФd, lntra-AllerФd Cold Period (1ACP) and Younger Dryas (YD). The summer monsoon was weaker during Oldest Dryas and Younger Dryas when the winter monsoon was stronger. However, during the B/A (BФlling/AllerФd) period, the summer monsoon strengthened, reflected by magnetic susceptibility, when the winter monsoon also became strong, which is different from the paleoclimatic pattern established in the East Asian monsoon region. Furthermore,the summer monsoon was nearly in phase with the climate changes inferred from the oxygen isotopic records of Greenland ice cores. It could be speculated that the variations of the sea ice cover in the high latitudes of the North Hemisphere affected the high pressure of Asian continent and the changes of the winter monsoon inland. On the other hand,the sea ice cover variations might have indirectly caused the occurrence of ENSO events that has tightly been related to the summer monsoon in northwest margin of East Asian monsoon region.     

USERS′ PERCEPTION OF KOWLOON PARK, HONG KONG： VISITING PATTERNS AND SCENIC ASPECTS

Hong Kong is a hyper-dense city with 7×10⁶ people living in an area of 1100km². One way to improve the livability of compacted and congested cities like Hong Kong is through the provision of urban parks, an aspect that has largely been under-researched. This study focuses on how users perceive and utilize various facilities in the Kowloon Park. The findings revealed that the Kowloon Park is one of the most preferred parks in Hong Kong for both local residents and tourists. Users were quite satisfied with the park’s facilities. Notably, the most important component of an urban park is its greenery. This is followed by water elements, seating places, and facilities for various recreational activities. The improvements users would like to see in urban parks include good design and management, meeting users’ needs, overcoming barriers to use, and providing a high quality and varied experience for different groups in the community. The findings of this study provide a good basis to address park management issues from the users’ perspective. In particular, parks should provide easy access, encourage optimum usage and enable complimentary improvements to the environment. Foundation item: Under the auspices of the Germany-Hong Kong Joint Research Scheme (No.GER/99-00/01) and the Faculty Research Grant of the Hong Kong Baptist University (No.FRG/00-01/I-38) Biography: WONG Koon-kwai (1950—), male, a native of Guangdong Province, Ph.D., associate professor, specialized in the analysis of human-environment interaction. E-mail: kenwong@hkbu.edu.hk     

THERMAL EFFECTS OF BUILDING＇S EXTERNAL SURFACES IN CITY——Characteristics of Heat Flux into and out of External Wall Surfaces

This study examined the thermal effects of building‘s external wall surfaces, using observational data of spatial-temporal distribution of surface temperature, air temperature, and heat flux into and out of external surface. Results indicate that external wall surface temperature and nearby air temperature vary with the change of orientation, height and season. In general, the external wall surface temperature is lower near the ground, and is higher near the roof, than nearby air temperature. But north wall surface temperature is mostly lower than nearby air temperature at the same height; south wall surface temperature during the daytime in December, and west wall surface temperature all day in August, is respectively higher than nearby air temperature. The heat fluxes into and out of external wall surfaces show the differences that exist in the various orientations, heights and seasons. In December,south wall surface at the lower sites emits heat and north wall surface at the higher sites absorbs heat. In April, all external wall surfaces, emit heat near the ground and absorb heat near the roof. In August, west wall surface all day emits heat, and other wall surfaces just show the commensurate behavior with that in April.     

ENVIRONMENTAL CHANGE AND ITS IMPACTS ON HUMAN SETTLF, MENT IN THE CHANGJIANG RIVER DELTA IN NEOLITHIC AGE

Dating data, altitude of Neolithic sites, climatic changes from sedimentary records and previous research results were collected and analyzed to detect possible connections between climatic changes and human activities in the Changjiang River Delta in the Neolithic Age. The results indicated that hydrological changes greatly impacted the human activities in the study region. Low-lying geomorphology made the floods and sea level changes become the important factors affecting human activities, especially the altitude change of human settlements. People usually moved to higher places during the periods characterized by high sea level and frequent floods to escape the negative influences from water body expansion, which resulted in cultural hiatus in certain profiles. However, some higher-altitude settlements were not the results of climatic changes but the results of social factors, such as religious ceremony and social status. Therefore, further research will be necessary for the degree and types of impacts of climatic changes on human activities in the study area at that time.     

VARIATIONAL ITERATION SOLVING METHOD FOR SEA-AIR OSCILLATOR MODEL OF INTERDECADAL CLIMATE FLUCTUATIONS

1IN T R O D U C T IO NInteractionsbetween the ocean and atmosphere con-tributeto climatefluctuationosver a broad spectrum oftime scales.Studiesof those interactionhsave farfo-cused on El Ni o-SouthernOscillation(ENSO)phe-nomenon thathas a period of3to4yea…     

HISTORICAL DESERTIFICATION PROCESS IN HEXI CORRIDOR, CHINA

1IN TR O D U CTIO NH istoricdaelsertificatiisonnotonlythekeyissueon theinteractiobnetweennatureandhuman proces,sbutalsothemost challenginigssueinthecomplex reltaionshipbetweenhuman andland.Previousl,ymostChinesesci-entistisnthisfieldcarrieoduttheiresearch…     

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Exopolysaccharide production by four cyanobacterial isolates and preliminary identification of these isolates

Four marine cyanobacterial isolates, named 104, 109, 113 and 115, from marine water off China＇s coast can release a large amount of exopolysaccharide （EPS） to medium. The effects of different components in medium on EPS production by the four isolates were investigated. Under the optimal condition, the EPS released by isolates 104,109, 113 and 115 reached 7.48 g L^-1, 8.33 g L^-1, 18.26 g L^-1, and 6.78 g L^-1 within 14 d,respectively. Based on the conventional identification methods for cyanobacteria,these isolates were assigned to genus Cyanothece.