Two GUIs-based analysis tool for spectroradiometer data pre-processing

A new graphical user interface (GUI) for pre-processing reflectance spectra, built using MATLAB and expressly designed for the ASD FieldSpec® spectroradiometer, was developed to solve problems that generally affect experimental ASD data. The GUI is characterised by an easily readable, graphic visualisation of spectra, from which the absorption band depth (ABD) can be obtained for a selected wavelength. The output format of the ASD data is a binary file with an .asd extension. The binary file, that provides a single spectrum, can be processed using a software functionality, by means of a GUI, that allows to select one or more binary files to produce a spectral library in a unique .txt file. The spectral reflectance is re-calibrated with the “convex-hull” methodology to eliminate the convex shape, which is typical of reflectance spectra. Different examples of the use of the new GUI are provided.     

A DNA tracer used in column tests for hydrogeology applications

Tracing techniques are commonly used to investigate groundwater quality and dynamics, as well as to measure the hydrogeological parameters of aquifers. The last decade has seen a growing interest in environmentally friendly tracers, including single-stranded DNA molecules. In this study, an electrolytic tracer and a synthetic DNA tracer are comparatively evaluated in laboratory scale tests to assess their potential application in field studies aimed at investigating groundwater environments. A real-time quantitative Polymerase Chain Reaction assay was developed and optimized to detect and quantify the DNA tracer, while tracer column tests were performed to investigate the DNA tracer behavior and to compare it to the electrolytic tracer. The results show that the DNA tracer has an almost pure convective flow, while the KCl tracer experiences dispersive behavior. The tracing method proposed can be applied in hydrogeological field studies involving calcareous fractured rock systems, with the DNA tracer particularly suitable in tracing karst systems, which are often characterized by several conduits of flow. To test the DNA tracer in operation, a preliminary test was conducted in the field.     

Bioremediation of petroleum hydrocarbons in anoxic marine sediments: Consequences on the speciation of heavy metals

We investigated the effects of biostimulation and bioagumentation strategies applied to harbor sediments displaying reducing conditions and high concentrations of petroleum hydrocarbons and heavy metals. We compared the microbial efficiency of hydrocarbon removal from sediments maintained for 60 days in anoxic conditions and inoculated with acetate, sulfate-reducing bacterial strains and acetate and sulfate-reducing bacteria. All treatments determined a significant increase in the microbial growth and significant decreases of hydrocarbon contents and of redox potential values. The addition of sulfate-reducing bacterial strains to the sediment was the most efficient treatment for the hydrocarbon removal. In all experiments, significant changes of the heavy metals’ phase repartition were observed. The results reported here suggest that the biodegradation of petroleum hydrocarbons in anoxic marine sediments may be enhanced by stimulating microbial anaerobic metabolism, but care should be applied to monitor the potential changes in the mobility and bioavailability of heavy metals induced by bio-treatments.     

Estimation of load reduction factors for clay masonry walls

The in‐plane cyclic behaviour of three types of unreinforced clay masonry was characterized by means of laboratory tests on full‐scale specimens. The masonry walls were assembled with various bonding arrangements (head joints made with mortar pockets, dry head joints with mechanical interlocking, thin‐layer mortar bed joints), which are not yet inserted in seismic codes. Experimental behaviour was modelled with an analytical hysteretic model able to predict lateral load–displacement curves in case of shear failure of the unreinforced walls. According to the experimental results and those of the selected analytical model, parametric study to evaluate the reduction in lateral strength demand produced by non‐linear behaviour in masonry walls, i.e. the load reduction factor was carried out by non‐linear dynamic analyses. The calculated values of the load reduction factor were modest. The differences in values found for the three masonry types, although consistent with them, were not great. This may indicate that, in the ultimate limit state, the type of masonry cannot significantly affect the behaviour of an entire building. Copyright © 2009 John Wiley & Sons, Ltd.     

The structure of the Campanian Plain and the activity of the Neapolitan volcanoes (Italy)

The central Campanian Plain is dominated by the structural depression of Acerra whose origin is tectonic, but may have been enlarged and further depressed after the eruption of the Campanian Ignimbrite (42-25 ka). The deposits of the Campanian Ignimbrite are possibly the results of multiple eruptions with huge pyroclastic deposits that covered all the Campanian Plain.The more recent activity of Vesuvius, Campi Flegrei and Procida occurred on the borders of Acerra depression and resulted from a reactivation of regional faults after the Campanian Ignimbrite cycle. The activity of Vesuvius produced the building of a stratovolcano mostly by effusive and plinian explosive eruptions. The Campi Flegrei area, on the contrary, was dominated by the eruption of the Neapolitan Yellow Tuff at 12 ka that produced a caldera collapse of the Gulf of Pozzuoli. The caldera formation controlled the emplacement of the recent activity of Campi Flegrei and the new volcanoes were formed only within the caldera or along its rim.     

New records of cold‐water coral sites and fish fauna characterization of a potential network existing in the Mediterranean Sea

New cold‐water coral (CWC) sites were recorded along the Apulian margin (Central Mediterranean). The species composition and depth distribution of CWCs were updated. A distribution of the CWC sites coincident with the course of the dense‐water masses that flow between the Southern Adriatic and Northern Ionian was confirmed. The faunal assemblages of five of these CWC sites were investigated and compared using experimental longlines during the spring–summer and autumn–winter seasons, between 2010 and 2014. Differences in ecological variables amongst the sites in each season were evaluated by means of a set of univariate and multivariate methods (analysis of variance, permutational multivariate analysis of variance, non‐metric multidimensional scaling). Although some differences were detected in relation to the different depths examined during spring–summer, the CWC sites showed similar features in terms of species richness and diversity as well as in the abundance of the same fish species (Galeus melastomus, Conger conger, Helicolenus dactylopterus, Merluccius merluccius, Phycis blennoides and Pagellus bogaraveo) most probably because of the distribution of adult specimens in structurally complex and heterogeneous habitats, which act as a potential ‘refuge network’ with respect to commercial fishing. The presence of maturing and mature individuals as well as post‐reproductive females indicates that these CWC sites also act as spawning areas, representing a potential ‘renewal network’ for the fish populations. The term ‘network’ used here refers to several similar subsystems (CWC sites) that play the same ecological role in a wider system (Apulian margin). These CWC communities need coherent conservation measures and management strategies according to the Ecosystem Approach to Fisheries.     

Time-course evaluation of ROS-mediated toxicity in mussels, Mytilus galloprovincialis, during a field translocation experiment

Harbours can be considered as model environments for developing and validating field monitoring procedures and to investigate mechanistic relationships between different biological responses. In this study several biomarkers were investigated in marine mussels caged for 4 weeks (June-July 2001) into an industrialized harbour of NW Italy. Organisms were collected at different time-intervals to better characterize the sensitivity, temporal variations and interactions of analysed responses. Besides single antioxidants the total oxyradical scavenging capacity (TOSC) assay was used to analyse the capability of the whole antioxidant system to neutralize specific forms of radicals: these data were further integrated by measurement of DNA integrity, oxidized bases and the impairment of lysosomal membrane stability in haemocytes. Results showed a biphasic trend for single antioxidants and TOSC, with an increase during the first 2 weeks of exposure to the polluted site followed by a progressive decrease up to a severe depletion in the final part of the experiment.