A variance of geostatisticians

Different individuals will take different approaches to the analysis and interpretation of data. This study attempted to quantify the effect of such individual differences on the quality of geostatistical spatial estimates. Identical spatial data sets were sent to 12 investigators, who independently analyzed the data and produced spatial interpolations. The results varied considerably. Differences in the interpolations could be attributed to differences in choice of methodology, differences in data interpretation, and, in a few cases, errors in procedure. The potential differences in economic and societal costs between decisions based on good vs. bad interpolations warrant a systematic approach to the identification and testing of interpolation methods.Although the research described in this article has been supported by the U.S. Environmental Protection Agency, it has not been subjected to Agency review and no official endorsement should be inferred.     

Effects of β-naphthoflavone on hepatic biotransformation and glutathione biosynthesis in largemouth bass (Micropterus salmoides)

We are investigating the effects of in vivo exposure of prototypical enzyme inducing agents on hepatic biotransformation enzyme expression in largemouth bass (Micropterus salmoides), a predatory game fish found throughout the United States and Canada. The current study targeted those genes involved in biotransformation and oxidative stress that may be regulated by Ah-receptor-dependent pathways. Exposure of bass to β-naphthoflavone (β-NF, 66 mg/kg, i.p.) elicited a 7–9-fold increase in hepatic microsomal cytochrome P4501A-dependent ethoxyresorufin O-deethylase (EROD) activities, but did not affect cytosolic GST catalytic activities toward 1-chloro-2,4-dinitrobenzene (CDNB) or 5-androstene-3,17-dione (ADI). Glutathione S-transferase A (GST-A) mRNA expression exhibited a transient, but non-significant increase following exposure to β-NF, and generally tracked the minimal changes observed in GST–CDNB activities. Expression of the mRNA encoding glutamate-cysteine ligase catalytic subunit (GCLC), the rate-limiting enzyme in glutathione (GSH) biosynthesis, was increased 1.7-fold by β-NF. Changes in GCLC mRNA expression were paralleled by increases in intracellular GSH. In summary, largemouth bass hepatic CYP1A-dependent and GSH biosynthetic pathways, and to a lesser extent GST, are responsive to exposure to β-NF.     

Conjugation of 4-hydroxynonenal by largemouth bass (Micropterus salmoides) glutathione S-transferases

The glutathione S-transferases (GST) are a major group of conjugative enzymes involved in the detoxification of electrophilic compounds and products of oxidative stress. We have previously described the kinetics of hepatic GST conjugation in largemouth bass using a variety of synthetic GST reference substrates. In the present study, we investigated the ability of largemouth bass hepatic GSTs to conjugate 4-hydroxynon-2-enal (4HNE), a mutagenic and cytotoxic alpha-beta-unsaturated aldehyde produced during oxidative injury. Hepatic cytosolic fractions from largemouth bass rapidly catalyzed GSH-dependent 4HNE conjugation, with the rate of GST-4HNE conjugation in bass liver exceeding those of several other mammalian and aquatic species. No apparent sex-related differences in GST-4HNE activity were observed among adult bass. SDS-PAGE and Western blotting analysis of GSH affinity-purified bass liver cytosolic GST revealed the presence of two major GST subunits of approximately 30 and 27 KDa that exhibited slight cross-reactivity when probed with a rat alpha class GST antibody, but not to rat mu, pi or theta class GST. The rapid conjugation of 4HNE by hepatic GST suggests an important role for GSTs in protecting against peroxidation of polyunsaturated fatty acids in bass liver.     

Long-term spatio-temporal precipitation variability in arid-zone Australia and implications for groundwater recharge

Quantifying dryland groundwater recharge as a function of climate variability is becoming increasingly important in the face of a globally depleted resource, yet this remains a major challenge due to lack of adequate monitoring and the complexity of processes involved. A previously unpublished and unique dataset of high density and frequency rainfall measurements is presented, from the Fowlers Gap Arid Zone Research Station in western New South Wales (Australia). The dataset confirms extreme spatial and temporal variability in rainfall distribution which has been observed in other dryland areas and is generally explained by the dominance of individual storm cells. Contrary to previous observations, however, this dataset contains only a few localised storm cells despite the variability. The implications of spatiotemporal rainfall variability on the estimation of groundwater recharge is assessed and show that the most likely recharge mechanism is through indirect and localised, rather than direct, recharge. Examples of rainfall and stream gauge height illustrate runoff generation when a spatially averaged threshold of 15–25 mm (depending on the antecedent moisture conditions) is exceeded. Preliminary assessment of groundwater levels illustrates that the regional water table is much deeper than anticipated, especially considering the expected magnitude of indirect and localised recharge. A possible explanation is that pathways for indirect and localised recharge are inhibited by the large quantities of Aeolian dust observed at the site. Runoff readily occurs with water collecting in surface lakes which slowly dry and disappear. Assuming direct groundwater recharge under these conditions will significantly overestimate actual recharge.     

Explaining agricultural collapse: Macro-forces,micro-crises and the emergence of land use vulnerability in southern Romania

Land use patterns influence the long-term productivity of agro-ecosystems and result from socio-economic as well as biophysical and climatic drivers. This paper qualitatively examines how past demographic and political-economic changes in southern Romania twice led to vulnerable socio-economic and agro-ecological systems. Drawing on this case study, we establish broad-brush hypotheses identifying some of the potential causes of vulnerability. We suggest that vulnerable systems appear more likely to occur when socio-political uncertainty combines with a lack of off-farm employment in regions where there are abundant natural capital stocks that can be quickly liquidated.     

Coping with food crises: Lessons from the American Dust Bowl on balancing local food,agro technology,social welfare,and government regulation agendas in food and farming systems

This paper examines four broad policy and public responses to the American Dust Bowl in the 1930s as a way of exploring how society today could address our own “food crisis”. More particularly, in the Dust Bowl some argued that solutions would be found by engineers and farmers who would develop new ways of watering dry fields. A second group believed that inappropriate political and economic incentives had led to bad farming practices prior to the drought and this contributed to wide-spread erosion. To this group, the best solutions included governmental regulation of farm practices. A third group focused on the welfare of individuals, arguing that creating a social safety net to protect marginalized families was the highest priority. Finally, there were commentators who advocated for a smaller scale, ecological, and holistic approach to farming. Today, the same four perspectives are present in debates about how to maintain global food security in light of population growth, high energy prices and climate change. Exploring the similarity of the discourses between today's food crisis and the one that hit American society 80 years ago reveals that advocates of the four different camps are motivated by very different principles. Briefly, proponents of technological solutions base many of their arguments on the assumption that human ingenuity is capable of producing extremely productive food systems. The managerial arguments, by contrast, are based on the idea that the natural environment can be rationally and efficiently managed using scientific principles. The social welfare narrative seeks to create a more equitable food system. Finally, the ecological and holistic narrative argues that diverse, small scale and local food systems are a prerequisite for long term sustainability. The primary contribution of this paper, therefore, is to expose these deeply held ontological tensions as a way of arguing that policy makers today must de-politicise arguments and use the principles embedded in all four narratives when designing programmes to ensure that the 21st century does not face a repeat of the crisis of the 1930s.