RP-HPLC法同时测定翅碱蓬中四种黄酮化合物的含量

建立RP-HPLC(反相高效液相色谱法)同时测定翅碱蓬中四种黄酮化合物-芸香苷、槲皮素3-β-D-葡萄糖苷、水仙苷和槲皮素的含量。采用Kromasil C18色谱柱(4.6 mm×250 mm,5μm),检测波长为361nm,柱温为30℃,流动相为甲醇-0.4%冰乙酸(55︰45),流速1.0mL/min,四种黄酮类化合物得到很好的分离。芸香苷、槲皮素3-β-D-葡萄糖苷、水仙苷和槲皮素浓度均为0.001~0.01 mg/mL范围内呈现良好线性关系,相关系数R2在0.9990~0.9996,加标回收率(n=3)范围为82.39%~99.03%。该方法简单、快速、高效,可以用于翅碱蓬中四种黄酮化合物的含量分析。     

恢复图们江出海权对东北亚区域经济政治和我国东北地区的影响和对策

为充分发挥图们江特殊地理位置的重要作用,促进国际和国内区域发展,文章分析恢复图们江出海权对东北亚区域经济政治和我国东北地区的影响,并提出对策建议。研究结果表明：我国恢复图们江出海权具有法理依据和现实需求;恢复图们江出海权对东北亚区域的能源和资源合作开发、中蒙俄经济走廊建设、环日本海国家经济合作以及东北亚区域政治格局具有重要影响,同时有利于我国东北地区维护边境地区安全、促进海洋经济发展、振兴东北老工业基地、打造海运通道和平衡区域经济发展;我国应抓住历史机遇,积极借助国际组织力量、对接各国发展需求以及以经济发展取代政治分歧,尽快恢复图们江出海权,为国际和国内区域发展提供保障。     

Analysis of resistance factors for LRFD of soil nail pullout limit state using default FHWA load and resistance models

Abstract

Resistance factors for load and resistance factor design (LRFD) of pullout limit state of both permanent and temporary soil nails are calibrated against a wide design space using the current Federal Highway Administration (FHWA) nail load and resistance models. The calculated resistance factors were shown to scatter broadly among design scenarios that differ in wall face batter, soil friction angle, nail ultimate bond strength, and surcharge live load. An important lesson learned from the analysis results is that the current practice of using a single resistance factor for LRFD of nail pullout limit state could not result in uniform reliabilities across different design scenarios. Simple artificial neural network (ANN) models were developed for computation of resistance factors. Design examples demonstrated the ability of the ANN models in providing resistance factors that yield satisfactory and consistent reliabilities in different nail pullout designs.     

临时潮位站实时潮位质量控制方法研究

为满足水上施工对临时潮位站实时潮位数据质量的要求,研究使用基于最小二乘的二次函数曲线拟合法将离散的潮位点拟合为潮位曲线,在此基础上实现潮位数据的补缺、加密和外推。在拟合节点的选取上除了传统的固定节点以外,还增加了滚动节点的拟合方式。以连云港徐圩潮位站的实测水位数据为依据,在不同的时刻计算拟合曲线,并给出了两种拟合节点的拟合外推潮位与实测潮位的比对分析结果,结果表明,滚动节点的拟合方式可以使拟合曲线的外推时间有所延长,在各种起始位置和节点质量条件下,在不超过20 cm偏差的前提下,潮位外推时间可达到50～300 min。     

ASO-S卫星工程科学应用系统的数据库设计

先进天基太阳天文台(Advanced Space-based Solar Observatory, ASO-S)卫星是我国首颗太阳观测卫星, 主要观测太阳耀斑和日冕物质抛射以及产生它们的磁场结构. ASO-S卫星的科学应用系统是科学卫星工程的6大系统之一, 它连接科学用户和卫星数据, 为将卫星的科学数据转化为科学成果提供保障. 科学应用系统的数据库是连接软件与海量数据的枢纽, 为科学数据生产和用户服务及运行提供数据层的支撑. 介绍了科学应用系统的数据库架构设计、数据库的选择以及数据库性能优化和表样例. 这里的数据库包括观测计划、工程参数、运维日志、科学数据、定标数据和特征事件识别等数据库. 这些数据库的建设将为ASO-S卫星工程科学应用系统的顺利运行提供数据支撑, 也可以为未来其他科学卫星类似数据库的搭建提供参考和借鉴.     

The sulfurization recorded in tridymite in the monomict eucrite Northwest Africa 11591

Some of the tridymite in the monomict Northwest Africa (NWA) 11591 eucrite are found to have sulfide‐rich replacement textures (SRTs) to varying degrees. The SRTs of tridymite in NWA 11591 are characterized by the distribution of loose porous regions with aggregates of quartz and minor troilite grains along the rims and fractures of the tridymite, and we propose a new mechanism for the origin of this texture. According to the volume and density conversion relationship, the quartz in the SRT of tridymite with a hackle fracture pattern was transformed from tridymite. We suggest that the primary tridymite grains are affected by the S‐rich vapors along the rims and fractures, leading to the transformation of tridymite into quartz. In addition, the S‐rich vapors reacted with Fe²⁺, which was transported from the relict tridymite and/or the adjacent Fe‐rich minerals, and/or the S‐rich vapors react with the exotic metallic Fe to form troilite grains. The sulfurization in NWA 11591 most likely occurred during the prolonged subsolidus thermal metamorphism in the shallow crust of Vesta and might be an open, relatively high temperature (>800 °C) process. Sulfur would be an important component of the metasomatic fluid on Vesta.     

三江源国家公园生态系统服务时空变化及权衡-协同关系

本文基于“当量因子法”、空间自相关及相关系数等方法,定量分析了1990—2015年三江源国家公园生态系统服务的时空变化及生态系统服务间的权衡与协同关系。结果表明：① 1990—2015年,三江源国家公园生态系统服务价值呈不断上升趋势,从研究初期的542.37亿元增加至研究末期的834.70亿元,增长292.33亿元,增幅53.90%,每一阶段平均增长率为9.01%;长江源园区与黄河源园区生态系统服务价值占比情况与三江源国家公园整体情况一致,水域的生态系统服务价值最高,其次为草地、湿地,其中草地与水域提供了各园区90%以上的生态服务;澜沧江源园区的生态系统服务占比则为草地（70.58%）、湿地（23.58%）、水域（3.38%）。② 空间上,长江源园区及澜沧江源园区的生态系统服务价值呈东南高西北低的特征;黄河源园区则呈现西高东低特征。③ 相关分析表明,协同关系是三江源国家公园生态系统服务之间的主导关系,但各生态系统服务之间的相关程度各异。④ 数量方面,4类典型生态服务之间的权衡与协同关系的Moran′s I指数与相关性系数表现一致;空间分析方面,生态系统服务之间权衡与协同关系的异质性显著。     

京津冀地区耕地利用转型时空分异及驱动机制

耕地利用转型代表耕地利用形态的趋势性变化。论文以京津冀地区157个县域空间为基本研究单元,从显性形态与隐性形态双重属性出发构建耕地利用形态指标体系,运用冷热点、空间变差函数等方法分析1990—2015年耕地利用形态变化的时空特征,在此基础上,利用空间误差模型(spatial error model, SEM)对耕地利用转型的驱动机制进行了定量分析。结果表明：① 京津冀地区耕地利用形态指数空间分布呈东南高、西北低的格局,与该地区地形分布格局基本一致,且因坝上高原特色农业发展形成独立高值区。② 京津冀地区耕地利用形态指数空间分异呈现逐渐缩小的趋势,显著热点区与显著冷点区分布相对稳定且面积不断缩小,耕地利用转型较为平稳。显性形态值普遍呈现先升高后降低趋势,隐性形态值则呈升高趋势,表明京津冀耕地出现空间收缩、功能优化式转型。③ 高程是影响京津冀地区耕地利用形态空间特征的主要自然环境因素,二三产业占比、地均固定资产投资与城镇化率等经济发展和城镇建设因素则是驱动1990—2015年京津冀地区耕地转型的主导因素,但不同时段、不同县域单元耕地转型动力机制存在差异,各驱动因子作用强度也不完全一致。     

Population,urbanization and economic scenarios over the Belt and Road region under the Shared Socioeconomic Pathways

The countries throughout the Belt and Road region account for more than 60% of the world's population and half of the global economy. Future changes in this area will have significant influences on the global economic growth, industrial structure and resource allocation. In this study, the proportion of the urban population to the total population and the gross domestic product were used to represent the levels of urbanization and economic development, respectively. The population, urbanization and economic levels of the Belt and Road countries for 2020–2050 were projected under the framework of the IPCC's shared socioeconomic pathways(SSPs), and the following conclusions are drawn.(1) The population, urbanization and economic levels in the Belt and Road region will likely increase under all five pathways. The population will increase by 2%–8%/10 a during 2020–2050 and reach 5.0–6.0 billion in 2050. Meanwhile, the urbanization rate will increase by 1.4%–7.5%/10 a and reach 49%–75%. The GDP will increase by 17%–34%/10 a and reach 134–243 trillion USD.(2) Large differences will appear under different scenarios. The SSP1 and SSP5 pathways demonstrate relatively high urbanization and economic levels, but the population size is comparatively smaller; SSP3 shows the opposite trend. Meanwhile, the economy develops slowly under SSP4, but it has a relatively high urbanization level, while SSP2 exhibits an intermediate trend.(3) In 2050, the population will increase relative to 2016 in most countries, and population size in the fastest growing country in Central Asia and the Middle East countries will be more than double. Urbanization will develop rapidly in South Asia, West Asia and Central Asia, and will increase by more than 150% in the fastest growing countries. The economy will grow fastest in South Asia, Southeast Asia and West Asia, and increase by more than 10 times in some counties with rapid economic development.     

Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性,属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高,α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高,且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸,并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示,α螺旋和β片层围成了蛋白酶的催化腔,EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体,且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性,并有助于其催化功能的发挥,为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。