Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of heavy metal exposure

Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.     

几种无色硅藻的生理特性及其细胞微细结构的研究

海洋中绝大多数硅藻细胞都有叶绿体,且能利用各种无机盐进行光合作用,称为光合硅藻。但也有不少硅藻尚能利用各种有机物质进行异养生活,据报道约50种左右。其中少数几种完全缺少光合色素,不能进行光合作用,只能利用有机碳或有机氮进行专性异养生活。它们主要生活于沿岸潮间带,特别附生于各种多细胞海藻体上。这类单细胞硅藻,种类不多,但为世界广分布的种类。到目前为止,对无色硅藻的研究报道还很少。本文第一作者在分离培养一种无色硅藻Nitzschia alba Lewin and Lewin作     

等离子体技术及其在地质上的应用

激光烧蚀微探针等离子体质谱的地质应用。自1989年以来，我们实验室就积极开展了激光烧蚀微探针(LAM)试样引入系统的研究工作，这种技术适合于ICP—MS分析地质样品中小体积矿物(激光烧蚀孔穴直径低于10—100μm)．对某些样品．采用Nd：YAG激光的基本IR波长(1064nm)，作为多数用途，4倍频率(uv)的波长(266nm)能产生较小的直径，并较好地形成孔穴．     

杭州湾南岸潮滩的~(210)Pb分布及其沉积学意义

杭州湾南岸潮滩的~(210)Pb垂向分布具有随深度波动的特征。湖滩颗粒物在吸附模拟系统中的~(210)Pb分配系数,主要受颗粒物含量的影响。本文从潮滩沉积~(210)Pb初始比度在低于平衡点一侧波动的机理,以及被沉积间断所分隔的有效封闭段的存在出发,提出选择常态沉积层的高~(210)Pb比度窗口,建立以CIC模式估计潮滩沉积速率的方法。     

Marine mammals from northeast Atlantic: relationship between their trophic status as determined by delta13C and delta15N measurements and their trace metal concentrations

The relationship between trophic position through delta13C and delta15N and trace metal concentrations (Zn, Cd, Cu and Hg) was investigated in the tissues of six marine mammal species from the Northeast Atlantic: striped dolphin Stenella coeruleoalba, common dolphin, Delphinus delphis, Atlantic white-sided dolphin Lagenorhynchus acutus, harbour porpoise Phocoena phocoena, white beaked-dolphin Lagenorhynchus albirostris, grey seal Halichoerus grypus stranded on French Channel and Irish coasts. White-beaked dolphins, harbour porpoises, white-sided dolphins, common and striped dolphins display the same relative and decreasing trophic position, as measured by delta15N values, along both the Irish and French channel coasts, reflecting conservative trophic habits between these two places. Hepatic and renal Cd concentrations were significantly correlated to muscle delta13C and delta15N values while Hg, Zn and Cu did not. These results suggest that Cd accumulation is partly linked to the diet while other factors such as age or body condition might explain Hg, Zn or Cu variability in marine mammals. Combined stable isotope and trace metal analyses appear to be useful tools for the study of marine mammal ecology.     

广州市地面塌陷分布特征与人为致灾因子分析

广州市地面塌陷频繁发生,近10年来呈波动式上升趋势。其塌陷类型分为岩溶塌陷和工程地面塌陷两种,岩溶塌陷主要分布在广花盆地的花都区、白云区,工程地面塌陷主要分布在主城区。地面塌陷孕灾环境复杂、致灾因子多样,承灾体脆弱,灾害后果严重,人为因素是地面塌陷的主要诱发因素。岩溶塌陷主要是由于过量抽取地下水或矿山疏干排水、地下采空、暴雨触发所致;工程塌陷主要是人类工程行为所致,其主要致灾因子包括排水疏干与突水（突泥）作用、人工加载、人工振动、人工开挖桩基、地表渗水、地铁等地下工程盾构掘进等。