Effects of heavy metal ions (Cu2+, Pb2+ and Cd2+) on DNA damage of the gills, hemocytes and hepatopancreas of marine crab, Charybdis japonica

There are rising concerns about the hazardous effects of heavy metals on the environment. In this study, comet assay and DNA alkaline unwinding assay were conducted on the tissues (gills, hepatopancreas, and hemocytes) of Charybdis japonica in order to illustrate genotoxicity of three heavy metal ions (Cu2+, Pb2+, and Cd2+) on the marine crabs C. japonica. The crabs were exposed to Cu2+ (10, 50, and 100 ?g.L?1), Pb2+ (50, 250, and 500 ?g L?1) and Cd2+ (5, 25, and 50 ?g L?1), and the tissues were sampled at days 0.5, 1, 3, 6, 9, and 15. DNA alkaline unwinding assay was used for testing the DNA single strand break in gills and hepatopancreas and comet assay was employed for testing the DNA damage in hemocytes. The results showed that the DNA damage (F-value) of gills in the crabs exposed to the three heavy metals was decreased gradually during the exposure periods and there was a dose-time response relationship in certain time, suggesting that the levels of DNA single strand break in all the experimental groups increased significantly compared to the controls. Changes of F-value in hepatopancreas of the crabs exposed to the three heavy metals were similar to those in gills except that the peak values were found in the 500 ?g L?1 Pb2+ treatment group at day 3 and the 50 ?g L?1 Cd2+ treatment group at day 9. The ranks of DNA damage in gills and hepatopancreas induced by the three heavy metal ions (50 ?g L?1, day 15) were Cd2+ >Pb2+ >Cu2+ and Pb2+ >Cu2+ >Cd2+. The levels of DNA damage in gills were higher than those in hepatopancreas in the same experimental group. It can be concluded that indices of DNA damage can be used as the potential biomarkers of heavy metal pollution in marine environment.     

滨海地区地下水泄流研究进展

20世纪90年代以来出现的对滨海地下水的研究方式取得了有意义的结果.其中W.S.Moore通过对226Ra富集揭示了地下水对海岸水体大量补给的研究以及Jacob、Sun等关于潮夕动力地下水流法的研究对解决环境地质问题具有一定的意义.     

六盘水市煤炭资源开发利用结构调整与优化的探讨

六盘水市矿产资源丰富,尤以煤炭为主,但从目前煤炭资源开发利用现状来看,其结构布局尚欠合理,开发利用方式比较粗放,存在＂采富弃贫、大矿小开、一矿多开、技术落后、安全条件差、资源回收率低＂等现象,环境保护与恢复治理的难度也越来越大。为了提高煤炭资源开采利用效率,从规模结构、技术结构、产品结构三个方面提出了六盘水市煤炭资源开发利用结构调整与优化方法：要求新建矿山在30万t/a以上,最低服务年限不低于25年,将目前的小型煤矿山减少到125个;采用高新技术和先进技术,促进矿企产业升级;进行产品结构调整,改变采矿、选矿、冶炼方式,实现集约型经营。     

Trace metals in porewater of surface sediments and their bioavailability in Jiaozhou Bay,Qingdao, China

Samples of porewater and the edible tissue of mottled clams, Ruditapes variegatus, were collected simultaneously at each of 12 stations from Jiaozhou Bay near Qingdao, China, in June of 2003. Chemical analysis focused on trace metals and major elements. Porewater concentrations of Cd, Cr, Pb, and Zn were distributed in a bimodal fashion among the 12 stations, with mean concentrations at six of the stations being 8–32 times mean values at the other six stations. The concentrations of the same metals in clams were remarkably similar among stations, the coefficients of variation being only 12–37%. Calculations performed with the computer program PHREEQC indicated that Pb and Cr in porewater were present only in the +2 and +3 states, respectively, and because dissolved Cd and Zn exist only in the +2 state, the bimodal distribution of these four metals likely reflects secondary effects associated with their scavenging by Fe and/or Mn under oxidizing conditions and subsequent dissolution in the reducing environment of the porewaters. Consistent with this hypothesis is the fact that the distribution of high and low metal concentrations was closely correlated with the granularity of the sediment, with lower metal concentrations associated with relatively coarse sediment. Comparison of published biological concentration factors with the ratios of metal concentrations in the clams to porewater metal concentrations indicated that the porewaters were not the primary sources of the metals in the clams. Mixing processes in the bay likely account for the rather uniform concentrations of metals in clam tissue.     

Characteristics of winter mass balance of Glacier No.1 at the headwaters of the Urumqi River,Tianshan Mountains

This study analyzes the response of glacier to climate change during the past 49 years in Urumqi River source region, the Tianshan Mountains of China. The temporal and spatial variations of winter mass balance (bn-w) at different time scales were analyzed to identify their response to climate change during 1988–2006 (The observation of winter mass balance observation began in 1988) on the Glacier No.1 at the headwaters of the Urumqi River, Tianshan Mountains, China. The winter accumulation shows a significantly decreasing trend. The results show that the cumulative values on Glacier No.1 is 2,202 mm water equivalent during 1988–2006 and the mean values is 116 mm a⁻¹. Furthermore, the trend analysis of the winter mass balance indicates a rapid decrease since 1990, and the mean mass balance is only 79 mm a⁻¹ during 1997–2006. Winter mass balance correlates well negatively with the total evaporation from September to April (r = −0.68, α = 0.01), and positively with the total precipitation from September to April (r = 0.74, α = 0.01). However, winter mass balance shows a weak correlation with mean minimum air temperature during September to April (r = −0.35), and runoff on September (r = −0.13).     

Shallow water depth retrieval from space-borne SAR imagery

Based on shallow water bathymetry synthetic aperture radar (SAR) imaging mechanism and the microwave scattering imaging model for oceanic surface features, we developed a new method for shallow water depth retrieval from space-borne SAR images. The first guess of surface currents and winds are estimated from the normalized radar crossing section (NRCS) profile of shallow water bathymetry SAR imagery, according to the linear theory and geophysical model function. The NRCS profile is then simulated by the microwave scattering imaging model. Both the surface currents and winds are adjusted by using the dichotomy method step by step to make the M4S-simulated NRCS profiles approach those observed by SAR. Then, the surface currents and the wind speeds are retrieved when a best fit between simulated signals and the SAR image appears. Finally, water depths are derived using the Navier–Stokes equation and finite difference method with the best estimated currents and the surface winds. The method is tested on two SAR images of the Taiwan Shoal. Results show that the simulated shallow water NRCS profile is in good agreement with those measured by SAR with the correlation coefficient as high as 85%. In addition, when water depths retrieved from the SAR image are compared with in situ measurements, both the root mean square and relative error are less than 3.0 m and 6.5%, respectively, indicating that SAR images are useful for shallow water depth retrieval and suggesting that the proposed method in this paper is convergent and applicable.     

Robust control based on feedback linearization for roll stabilizing of autonomous underwater vehicle under wave disturbances

In the case of Autonomous Underwater Vehicle(AUV) navigating with low speed near water surface,a new method for design of roll motion controller is proposed in order to restrain wave disturbance effectively and improve roll stabilizing performance.Robust control is applied,which is based on uncertain nonlinear horizontal motion model of AUV and the principle of zero speed fin stabilizer.Feedback linearization approach is used to transform the complex nonlinear system into a comparatively simple linear system.For parameter uncertainty of motion model,the controller is designed with mixed-sensitivity method based on H-infinity robust control theory.Simulation results show better robustness improved by this control method for roll stabilizing of AUV navigating near water surface.     

Environmental capacity of chemical oxygen demand in the Bohai Sea: modeling and calculation

A three-dimensional advection-diffusion model coupled with the degradation process is established for describing the transport of chemical oxygen demand (COD). Comparison of the simulated distribution of COD at the surface in the Bohai Sea in August, 2001 with field observations, shows that the model simulates the dataset reasonably well. The Laizhou Bay, Bohai Bay, and Liaodong Bay were contaminated heavily near shore. Based on the optimal discharge flux method, the Environmental Capacity (EC) and allocate...     

Impact of land use change on groundwater recharge in Guishui River Basin,China

It is important to understand how land use change impacts groundwater recharge,especially for regions that are undergoing rapid urbanization and there is limited surface water.In this study,the hydrological processes and re-charge ability of various land use types in Guishui River Basin,China(in Beijing Municipality) were analyzed.The impact of land use change was investigated based on water balance modeling,WetSpass and GIS.The results indicate that groundwater recharge accounts for only 21.16% of the precipitation,while 72.54% is lost in the form of evapotranspiration.The annual-lumped groundwater recharge rate decreases in the order of cropland,grassland,urban land,and forest.Land use change has resulted in a decrease of 4 × 106 m3 of yearly groundwater recharge in the study area,with a spatially averaged rate of 100.48 mm/yr and 98.41 mm/yr in 1980 and 2005,respectively.This variation has primarily come from an increase of urban area and rural settlements,as well as a decrease of cropland.     

基于DSP6000的实验室安全控制系统研究

以TMS320DM642为核心构建一个实验室安全控制系统,通过摄像机采集视频图像,将图像传输给DM642进行图像处理,包括获取背景图像、差影处理、图像分割、运动目标检测、安全控制,并将处理结果显示在液晶屏上.实验验证,系统能够实现实验室条件下的安全控制.