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71.
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采用菌丝 原位包埋脉冲电泳的方法从链霉菌FR-008中分离出三条DNA带,双向电泳及脉冲条件变换实验证明,这些DNA带都是一分子外切核酶酶酶解发现,它们的5‘末端封闭。这三条DNA带分别代表了链霉菌FR-008中线性染色体和两条线性质粒。 相似文献
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大西洋经向翻转环流(Atlantic Meridional Overturning Circulation,AMOC)是气候系统重要的组成部分,其强度变化可直接影响南北半球的热量分配,厘清其变化机理对全球变暖背景下的未来预估至关重要。海洋沉积物记录发现,在晚更新世,AMOC的变化与地球岁差周期有紧密联系,但其物理机理尚不清楚。本文利用海洋−大气耦合气候模型—COSMOS(ECHAM5/JSBACH/MPIOM)模型,通过敏感试验,分析在冰盛期冷期和间冰期暖期气候背景下,AMOC对地球岁差变化的响应机理。结果表明:岁差降低引起的北半球夏季太阳辐射增强,会导致间冰期暖期背景下的AMOC显著减弱,但对冰盛期AMOC的影响并不明显。通过进一步分析发现,在间冰期暖期,夏季太阳辐射增强,造成高低纬大西洋海表的升温,同时促进北大西洋高纬度地区的局地降水,两者导致北大西洋表层海水密度降低,共同削弱大西洋深层水生成。而在冰盛期冷期,大西洋高低纬度地区的响应对AMOC的影响反向—副热带升温触发的海盆尺度低压异常,通过其南侧的西风异常削弱大西洋向太平洋的水汽输送,导致净降水增多,海表盐度下降;同时,高纬度升温造成的海冰减少,促进了海洋热丧失,海表失热变重,有利于大西洋深层水的生成,最终两者的共同作用导致AMOC对岁差变化的响应偏弱。本文系统揭示了不同气候背景下,岁差尺度AMOC变化的控制机理,对理解晚更新世AMOC重建记录中持续存在的岁差周期具有重要启示意义。 相似文献
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We describe the microfabrication of 85Rb vapour cells using a glass-silicon anodic bonding technique and in situ chemical reaction between rubidium chloride and barium azide to produce Rb. Under controlled conditions, the pure metallic Rb drops and buffer gases were obtained in the cells with a few mm3 internal volumes during the cell sealing process. At an ambient temperature of 90 ℃ the optical absorption resonance of 85Rb D1 transition with proper broadening and the corresponding coherent population trapping (CPT) resonance, with a signal contrast of 1.5% and linewidth of about 1.7 kHz, have been detected. The sealing quality and the stability of the cells have also been demonstrated experimentally by using the helium leaking detection and the after-9-month optoelectronics measurement which shows a similar CPT signal as its original status. In addition, the physics package of chip-scale atomic clock (CSAC) based on the cell was realized. The measured frequency stability of the physics package can reach to 2.1×10-10 at one second when the cell was heated to 100 ℃ which proved that the cell has the quality to be used in portable and battery-operated devices. 相似文献
77.
A Wind stress–Current Coupled System (WCCS) consisting of the HYbrid Coordinate Ocean Model (HYCOM) and an improved wind stress algorithm based on Donelan et al. [Donelan, W.M., Drennan, Katsaros, K.B., 1997. The air–sea momentum flux in mixed wind sea and swell conditions. J. Phys. Oceanogr. 27, 2087–2099] is developed by using the Earth System Modeling Framework (ESMF). The WCCS is applied to the global ocean to study the interactions between the wind stress and the ocean surface currents. In this study, the ocean surface current velocity is taken into consideration in the wind stress calculation and air–sea heat flux calculation. The wind stress that contains the effect of ocean surface current velocity will be used to force the HYCOM. The results indicate that the ocean surface velocity exerts an important influence on the wind stress, which, in turn, significantly affects the global ocean surface currents, air–sea heat fluxes, and the thickness of ocean surface boundary layer. Comparison with the TOGA TAO buoy data, the sea surface temperature from the wind–current coupled simulation showed noticeable improvement over the stand-alone HYCOM simulation. 相似文献
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We conducted studies of phytoplankton and hydrological variables in a semi-enclosed bay in northern China to understand the spatial–temporal variability and relationship between these variables. Samples were collected during seven cruises in Jiaozhou Bay from November 2003 to October 2004, and were analyzed for temperature, nutrients and phytoplankton pigments. Pigments from eight possible phytoplankton classes (Diatoms, Dinoflagellates, Chlorophyceae, Prasinophyceae, Chrysophyceae, Haptophyceae, Cryptophyceae and Caynophyceae) were detected in surface water by high performance liquid chromatography (HPLC). Phytoplankton pigment and nutrient concentrations in Jiaozhou Bay were spatially and temporally variable, and most of them were highest in the northern and eastern parts of the sampling regions in spring (May) and summer (August), close to areas of shellfish culturing, river estuaries, dense population and high industrialization, reflecting human activities. Chlorophyll a was recorded in all samples, with an annual mean concentration of 1.892 μg L−1, and fucoxanthin was the most abundant accessory pigment, with a mean concentration of 0.791 μg L−1. The highest concentrations of chlorophyll a (15.299 μg L−1) and fucoxanthin (9.417 μg L−1) were observed in May 2004 at the station close to the Qingdao Xiaogang Ferry, indicating a spring bloom of Diatoms in this area. Although chlorophyll a and other biomarker pigments showed significant correlations, none of them showed strong correlations with temperature and nutrients, suggesting an apparent de-coupling between the pigments and these hydrological variables. The nutrient composition and phytoplankton community composition of Jiaozhou Bay have changed significantly in the past several decades, reflecting the increasing nutrient concentrations and decline of phytoplankton cell abundance. The unchanged total chlorophyll a levels indicated that smaller species have filled the niche vacated by the larger species in Jiaozhou Bay, as revealed by our biomarker pigment analysis. 相似文献
80.
Cui-Ping Kuang Si-Yu Chen Yu Zhang Jie GuLing Deng Yi PanJing Huang 《Coastal Engineering》2012,59(1):14-27
This paper is a companion of a two-part papers on the development of a two-dimensional morphological model—CurWaC2D-Sed, based on next generation circulation solver. In this paper, the model developed in paper I is applied to the large-scale construction of near-harbor industrial zone with deep water harbor by reclamation in Caofeidian, Bohai Bay, which is a typical sandbar-lagoon bay-type tidal inlet system. Characteristics of tides, currents, waves and sediments in Caofeidian are firstly analyzed with field data from comprehensive field observations carried out in 2008. These data are then used for model calibration and validation, showing a good agreement between simulated results and field measurements for tides, currents and suspended sediments. The verified model is applied to study the effects of the construction of Caofeidian embankment and the on-going engineering scheme on hydrodynamics and sediment transports as compared with no constructions under three representative tide conditions and their combinations with normal and strong waves as well as on morphology evolution. The simulated results indicate that the present scheme leads to morphological changes in Caofeidian with acceptable rates of erosion and sedimentation. However, some attentions should be paid to erosion at offshore wharf in front of Caofedian foreland which may lead to potentially increasing landslide risks due to steep slope and sedimentation in Laogonggou creek. 相似文献