Resolving mechanisms of toxicity while pursuing ecotoxicological relevance?

In this age of modern biology, aquatic toxicological research has pursued mechanisms of action of toxicants. This has provided potential tools for ecotoxicologic investigations. However, problems of biocomplexity and issues at higher levels of biological organization remain a challenge. In the 1980s and 1990s and continuing to a lesser extent today, organisms residing in highly contaminated field sites or exposed in the laboratory to calibrated concentrations of individual compounds were carefully analyzed for their responses to priority pollutants. Correlation of biochemical and structural analyses in cultured cells and tissues, as well as the in vivo exposures led to the production and application of biomarkers of exposure and effect and to our awareness of genotoxicity and its chronic manifestations, such as neoplasms, in wild fishes. To gain acceptance of these findings in the greater environmental toxicology community, “validation of the model” versus other, better-established often rodent models, was necessary and became a major focus. Resultant biomarkers were applied to heavily contaminated and reference field sites as part of effects assessment and with investigations following large-scale disasters such as oil spills or industrial accidents.

Over the past 15 years, in the laboratory, small aquarium fish models such as medaka (Oryzias latipes), zebrafish (Danio rerio), platyfish (Xiphophorus species), fathead minnow (Pimephales promelas), and sheepshead minnow (Cyprinodon variegatus) were increasingly used establishing mechanisms of toxicants. Today, the same organisms provide reliable information at higher levels of biological organization relevant to ecotoxicology. We review studies resolving mechanisms of toxicity and discuss ways to address biocomplexity, mixtures of contaminants, and the need to relate individual level responses to populations and communities.     

Searches for very high energy gamma rays from blazars with CANGAROO-III telescope in 2005-2009

We have searched for very high energy (VHE) gamma rays from four blazars using the CANGAROO-III imaging atmospheric Cherenkov telescope. We report the results of the observations of H 2356-309, PKS 2155-304, PKS 0537-441, and 3C 279, performed from 2005 to 2009, applying a new analysis to suppress the effects of the position dependence of Cherenkov images in the field of view. No significant VHE gamma ray emission was detected from any of the four blazars. The GeV gamma-ray spectra of these objects were obtained by analyzing Fermi/LAT archival data. Wide range (radio to VHE gamma-ray bands) spectral energy distributions (SEDs) including CANGAROO-III upper limits, GeV gamma-ray spectra, and archival data, even though they are non-simultaneous, are discussed using a one-zone synchrotron self-Compton (SSC) model in combination with a external Compton (EC) radiation. The HBLs (H 2356-309 and PKS 2155-304) can be explained by a simple SSC model, and PKS 0537-441 and 3C 279 are well modeled by a combination of SSC and EC model. We find a consistency with the blazar sequence in terms of strength of magnetic field and component size.     

Solar Microwave Bursts from Electron Populations with a 'Broken’ Energy Spectrum

Usually the gyrosynchrotron emission of microwave bursts from electron populations with a power-law (PL) energy distribution has been considered under the assumption that the spectral index of the distribution is constant over a wide range of energies. Meanwhile, there is strong evidence, in particular from hard X-ray and -ray, but also from cm/mm wavelength radio observations, that in many solar flare events the spectrum of the emitting electrons is characterized by a significant hardening at energies above 100–500 keV. We present some examples of calculated microwave burst spectra at cm/mm wavelengths taking into account the above evidence. It is shown that a break in the energy spectrum of the PL electrons can indeed result in a spectral hardening sometimes observed in microwave bursts at frequencies above 10–30 GHz.     

Gamma-Hadron Separation using Čerenkov Photon Density Fluctuations

In the atmospheric Čerenkov technique γ-rays are detected against the abundant background produced by hadronic showers. In order to improve the signal to noise ratio of theexperiment, it is necessary to reject a significant fraction of hadronic showers. Traditional background rejection methods based on image shape parameters have been extensively used for the data from imaging telescopes. However, non-imaging Čerenkov telescopes have to develop very different means of statistically identifying and removing cosmic ray events. Some of the parameters, which could be potentially important for non-imaging arrays, are the temporal and spectral differences, the lateral distributions and density fluctuations of Čerenkov photons generated by γ-ray and hadron primaries. Here we study the differences in fluctuations of Čerenkov photon density in the light pool at the observation level from showers initiated by photons and those initiated by protons or heavier nuclei. The database of simulated events for the PACT array has been used to evaluate the efficiency of the new technique. Various types of density fluctuations like the short range and medium range fluctuations as well as flatness parameter are studied. The estimated quality factors reflect the efficiencies with which the hadrons can be rejected from the data. Since some of these parameters are independent, the cuts may be applied in tandem and we demonstrate that the proton rejection efficiency of ∼90% can be achieved. Use of density fluctuations is particularly suited for wavefront sampling observations and it seems to be a good technique to improve the signal to noise ratio. This revised version was published online in July 2006 with corrections to the Cover Date.     

Persistent organic pollutants and histological lesions in Mayan catfish Ariopsis assimilis from the Bay of Chetumal, Mexico

Livers of catfish (Ariopsis assimilis) from the Bay of Chetumal were analyzed for organochlorine compounds and hydrocarbons as part of a study to diagnose the environmental health of the Bay after a catfish mass mortality that occurred in 1996. The presence of histological lesions in several organs of the fish as result of chemical exposure was also evaluated. The concentrations of organic pollutants found in the Bay may be considered high if compared to the levels reported for sites affected by chemical pollution. High prevalences of cellular alteration histopathologies were found in liver, including hepatic tumors. The presence of some lesions may be related statistically to environmental pollution in the Bay, specially with chlorinated compounds.     

Low induction number, ground conductivity meters: A correction procedure in the absence of magnetic effects

Ground conductivity meters, comprising a variety of coil–coil configurations, are intended to operate within the limits provided by a low induction number (LIN), electromagnetic condition. They are now routinely used across a wide range of application areas and the measured apparent conductivity data may be spatially assembled and examined/correlated alongside information obtained from many other earth science, environmental, soil and land use assessments. The theoretical behaviour of the common systems is examined in relation to both the prevailing level of subsurface conductivity and the instrument elevation. It is demonstrated that, given the inherent high level of accuracy of modern instruments, the prevailing LIN condition may require operation in environments restricted to very low (< 12 mS/m) conductivities. Beyond this limit, non-linear departures from the apparent conductivity that would be associated with a LIN condition occur and are a function of the coil configuration, the instrument height and the prevailing conductivity. Using both theory and experimental data, it is demonstrated that this has the potential to provide biased and spatially distorted measurements. A simple correction procedure that can be applied to the measured data obtained from any of the LIN instruments is developed. The correction procedure would, in the limit of a uniform subsurface, return the same (correct) conductivity, irrespective of the ground conductivity meter used, the prevailing conductivity or the measurement height.     

A comparison of the photospheric and interplanetary magnetic fields during the flight of Mariner IV

The polarity of the interplanetary magnetic field observed by the Mariner IV spacecraft is compared to the polarity of the photospheric magnetic field observed with the solar magnetograph at Mt. Wilson Observatory. Unlike the results obtained from observations during the flight of IMP-I, these polarities are not well correlated when the photospheric polarity is determined from data along a narrow latitudinal strip. It is suggested that the structure of the interplanetary field is often related to major features in the photospheric field that are observed over a broad range of solar latitudes.     

The geometry and petrogenesis of hydrothermal dolomites at Navan, Ireland

The dolomites at Navan, Ireland, formed in Courceyan peritidal and shallow-shelf limestones. The dolomite body has a plume-like geometry, cross-cutting both lithological boundaries and diagenetic barriers generated by sea-floor cementation and emergence. The dolomitizing fluids rose parallel to major faults to diffuse laterally through the succession, controlled by variations in permeability that reflect both facies variation on deposition and pre-dolomitization diagenesis. Cathodoluminescent zones reveal three principal stages of dolomite emplacement, separated by dissolution surfaces, with each stage reflecting several changes in the character of dolomitizing solutions. The predominance of dull zones indicates burial rather than surface conditions. The dolomites formed some time after burial in response to an areally limited hydrothermally-driven flow. Isotope values (σ¹⁸O of — 6σ6 to — 10.4%_δ and σ¹³C of — 0σ2 to +2σ5%_δ PDB), and fluid inclusion data, suggest that these fluids had compositions similar to those of Carboniferous seawater. However, these became hotter with time, with temperature increasing from 60 to 160δC. The Navan dolomites are closely associated with Europe's largest zinc-lead deposit. The distribution of the ores follows the same trend as that of the dolomites and paragenetic relationships indicate that dolomitization and mineralization were temporally and genetically related.     

TEM study of a special grain boundary in a synthetic K-feldspar bicrystal: Manebach Twin

 A monoclinic KAlSi₃O₈ feldspar Manebach twin boundary was synthesized by diffusion bonding and examined using high-resolution transmission electron microscopy. The sharp (001) twin boundary is straight and free of strain. The boundary width is smaller than d₀₀₁. There is no rigid body shift observed at the twin boundary, and the feldspar structure is arranged symmetrically across (001). The twin boundary structure consists of bridged tetrahedral crankshafts, which are characteristic of the feldspar lattice. The grain boundary structure is in good agreement with the geometrical model of Taylor et al. (1934). The grain boundary composition of K_1/2H_1/2AlSi₃O₈ differs from their model. Received: 13 February 2002 / Accepted: 24 December 2002 Acknowledgements We thank M. Rühle, S. Hutt, J. Mayer, A. Strecker and U. Salzberger at MPI, Stuttgart, for their support and valuable advice in preparing TEM sections of bicrystals.     

The OSO-1 solar neutron experiment

BF₃ counters on OSO-1 were used to look for solar neutrons by trying to observe a diurnal variation in count rate. No effect was observed and an upper limit was placed on the solar neutron flux at the earth of J _n < 2 × 10^–3 neut/cm²/sec of 10 keV < E _n < 10 meV for the period March to May 1962. No proton-producing flares occurred during this time, so the most obvious source of solar neutrons could not be studied. The emulsion experiment of Apparao et al., flown during this period, which seemed to indicate solar neutrons has probably been misinterpreted.