Microbial–silica interactions in Icelandic hot spring sinter: possible analogues for some Precambrian siliceous stromatolites

Silicified deposits, such as sinters, occur in several modern geothermal environments, but the mechanisms of silicification (and crucially the role of microorganisms in their construction) are still largely unresolved. Detailed examination of siliceous sinter, in particular sections of microstromatolites growing at the Krisuvik hot spring, Iceland, reveals that biomineralization contributes a major component to the overall structure, with approximately half the sinter thickness attributed to silicified microorganisms. Almost all microorganisms observed under the scanning electron microscope (SEM) are mineralized, with epicellular silica ranging in thickness from < 5 μm coatings on individual cells, to regions where entire colonies are cemented together in an amorphous silica matrix tens of micrometres thick. Within the overall profile, there appears to be two very distinct types of laminae that alternate repeatedly throughout the microstromatolite: ‘microbial’ layers are predominantly consisting of filamentous, intact, vertically aligned, biomineralized cyanobacteria, identified as Calothrix and Fischerella sp.; and weakly laminated silica layers which appear to be devoid of any microbial component. The microbial layers commonly have a sharply defined base, overlying the weakly laminated silica, and a gradational upper surface merging into the weakly laminated silica. These cyclic laminations are probably explained by variations in microbial activity. Active growth during spring/summer allows the microorganisms to keep pace with silicification, with the cell surfaces facilitating silicification, while during their natural slow growth phase in the dark autumn/winter months silicification exceeds the bacteria’s ability to compensate (i.e. grow upwards). At this stage, the microbial colony is probably not essential to microstromatolite formation, with silicification presumably occurring abiogenically. When conditions once again become favourable for growth, recolonization of the solid silica surface by free‐living bacteria occurs: cell motility is not responsible for the laminations. We have also observed that microbial populations within the microstromatolite, some several mm in depth, appear viable, i.e. they still have their pigmentation, the trichomes are not collapsed, cell walls are unbroken, cytoplasm is still present and they proved culturable. This suggests that the bulk of silicification occurred rapidly, probably while the cells were still alive. Surprisingly, however, measurements of light transmittance through sections of the microstromatolite revealed that photosynthetically active light (PAL) only transmitted through the uppermost 2 mm. Therefore the ‘deeper’ microbial populations must have either: (i) altered their metabolic pathways; (ii) become metabolically inactive; or (iii) the deeper populations may be dominated by different microbial assemblages from that of the surface. From these collective observations, it now seems unequivocal that microstromatolite formation is intimately linked to microbial activity and that the sinter fabric results from a combination of biomineralization, cell growth and recolonization. Furthermore, the similarities in morphology and microbial component to some Precambrian stromatolites, preserved in primary chert, suggests that we may be witnessing contemporaneous biomineralization processes and growth patterns analogous to those of the early Earth.     

Interactive Web-Based Map: Applications to Large Data Sets in the Geosciences

Current advances in computer hardware, information technology and data collection techniques have produced very large data sets in a wide variety of scientific and engineering disciplines. We must harness this opportunity to visualize and extract useful information from geophysical and geological data. We have taken the task of data mining by implementing a map-like approach over a web server for interrogating the data, using a client-server paradigm. The spatial-data is mapped onto a two-dimensional grid from which the user (client) can quiz the data with the map-interface as a user extension. The data is stored on the server, while the computational gateway separating the client and the server can be the front-end of an electronic publication, electronic classroom, a survey, or an e-business. We have used a combination of Java, Java3D, and Perl for processing the data and communicating between the client and the server. The user can interrogate the geospatial data over any particular region with arbitrary dimensions and then receive back relevant statistical analysis, such as the histogram plots and local statistics. We have applied this method for the following data sets: (1.) distribution of prime numbers (2.) two-dimensional mantle convection (3.) three-dimensional mantle convection (4.) high-resolution satellite reflectance data over multiple wavelengths (5.) molecular dynamics describing the flow of blood in narrow vessels. Using the map-interface, one can actually interrogate this data over the Internet.     

Reactivated strike–slip faults: examples from north Cornwall, UK

Several strike–slip faults at Crackington Haven, UK show evidence of right-lateral movement with tip cracks and dilatational jogs, which have been reactivated by left-lateral strike–slip movement. Evidence for reactivation includes two slickenside striae on a single fault surface, two groups of tip cracks with different orientations and very low displacement gradients or negative (left-lateral) displacements at fault tips.

Evidence for the relative age of the two strike–slip movements is (1) the first formed tip cracks associated with right-lateral slip are deformed, whereas the tip cracks formed during left-lateral slip show no deformation; (2) some of the tip cracks associated with right-lateral movement show left-lateral reactivation; and (3) left-lateral displacement is commonly recorded at the tips of dominantly right-lateral faults.

The orientation of the tip cracks to the main fault is 30–70° clockwise for right-lateral slip, and 20–40° counter-clockwise for left-lateral slip. The structure formed by this process of strike–slip reactivation is termed a “tree structure” because it is similar to a tree with branches. The angular difference between these two groups of tip cracks could be interpreted as due to different stress distribution (e.g., transtensional/transpressional, near-field or far-field stress), different fracture modes or fractures utilizing pre-existing planes of weakness.

Most of the d–x profiles have similar patterns, which show low or negative displacement at the segment fault tips. Although the d–x profiles are complicated by fault segments and reactivation, they provide clear evidence for reactivation. Profiles that experienced two opposite slip movements show various shapes depending on the amount of displacement and the slip sequence. For a larger slip followed by a smaller slip with opposite sense, the profile would be expected to record very low or reverse displacement at fault tips due to late-stage tip propagation. Whereas for a smaller slip followed by larger slip with opposite sense, the d–x profile would be flatter with no reverse displacement at the tips. Reactivation also decreases the ratio of d_max/L since for an original right-lateral fault, left lateral reactivation will reduce the net displacement (d_max) along a fault and increase the fault length (L).

Finally we compare Crackington Haven faults with these in the Atacama system of northern Chile. The Salar Grande Fault (SGF) formed as a left-lateral fault with large displacement in its central region. Later right-lateral reactivation is preserved at the fault tips and at the smaller sub-parallel Cerro Chuculay Fault. These faults resemble those seen at Crackington Haven.     

Palaeoecology and taxonomy of Pentoxylales from the Albian of Antarctica

Reproductive pentoxylalean material from the Albian Triton Point Formation, Fossil Bluff Group of Alexander Island, Antarctica is the youngest record of this group globally. Leaves are referred to Taeniopteris daintreei McCoy, ovulate reproductive organs to Carnoconites cranwelli Harris, and stem material to Pentoxylon sp. Occurring in leaf litter layers on palaeosol surfaces the sedimentology suggests that pentoxylalean plants grew upon areas of the floodplain distal to the river channel, where they covered the forest floor in a similar habit to modern brambles. The forest floor was shared with Equisetales and liverworts, an abundance of ferns [Cladophlebis Brongniart, Gleichenites Goeppert, Phyllopteroides Medwell, Sphenopteris (Brongniart) Sternberg], as well as angiosperms. Bennettitales, Ginkgoites Seward and Nilssonia Brongniart were rare within these communities, as were coniferous trees.     

Avoiding numerical stability problems of long duration DGPS/INS Kalman filters

A current pursuit of the geodetic community is the optimal integration of differential GPS (DGPS) and inertial navigation system (INS) data streams for precise and efficient position and gravity vector surveying. Therein a complete INS and multiple-antenna GPS receiver payload, mounted on a moving platform, is used in conjunction with a network of ground-fixed single antenna GPS receivers. This paper presents a complete, GPS-based, external updating measurement model for the applicable Kalman filter. The model utilizes four external observation types for every GPS satellite in-view: DGPS range differences, single phase differences, and single phase-rate differences; as well as the mobile, multipleantenna GPS receiver's measurement of theerrors in the INS's estimate of the phase difference between any two vehicle-borne GPS antennae. Although not widely conveyed in the geodetic world, the inertial navigation community has long known that traditional Kalman filter covariance propagation recurrences are inherently unstable when such highly accurate external updates are repeatedly applied (every 1 second) over long time durations. A hybrid square root covariance/U — D covariance factorization approach is a numerically stable alternative and is reviewed herein. The hybrid makeup of the algorithm is necessitated by the correlated nature of the fourth type of GPS external measurement listed above (each vehicle-borne GPS antenna formstwo baselines). Such measurement correlations require a functional transformation of the overall external updating model to permit the multiple updates (simultaneously available at each updating epoch) to be sequentially (and efficiently) processed. An appropriate transformation is given. Stable covariance propagation relationships are presented and the transformed Kalman gain is also furnished and its use in the determination of the externally updated error states is discussed. Specific DGPS/INS instabilities produced by the traditional recurrences are displayed. The stable alternative method requires about 25% more CPU time than the traditional Kalman recurrences. With the ever-increasing computational speeds of microprocessors, this added CPU time is of no real concern.     

Free cupric ion concentrations and Cu complexation in selected Swiss lakes and rivers

[Cu²⁺] and Cu complexation parameters in some selected freshwater systems in Switzerland were determined by the technique of ligand-exchange and DPCSV. Results from the water columns of some eutrophic and oligotrophic lakes are presented and compared to small acid lakes. Cu is strongly complexed by organic ligands which with very high stability constants at low concentrations are probably biologically produced, as indicated by the seasonal variations in the eutrophic lakes and by the relationship between Cu complexation and algal activity in the eutrophic (pCu=15–16), oligotrophic (pCu=13–14) and acidic (pCu=9–10) lakes. The extent of Cu complexation in river waters was generally lower than in the eutrophic lakes, at similar DOC levels. No obvious correlation between Cu complexation and DOC was observed, indicating that Cu complexing ligands are specific organic compounds.     

Petroleum Hydrocarbon Bioventing Kinetics Determined in Soil Core, Microcosm, and Tubing Cluster Studies

Aerobic biodegradation of vapor-phase petroleum hydrocarbons was evaluated in an intact soil core from the site of an aviation gasoline release. An unsaturated zone soil core was subjected to a flow of nitrogen gas, oxygen, water vapor, and vapor-phase hydrocarbons in a configuration analogous to a biofilter or an in situ bioventing or sparging situation. The vertical profiles of vapor-phase hydrocarbon concentration in the soil core were determined by gas chromatography of vapor samples. Biodegradation reduced low influent hydrocarbon concentrations by 45 to 92 percent over a 0.6-m interval of an intact soil core. The estimated total hydrocarbon concentration was reduced by 75 percent from 26 to 7 parts per million. Steady-state concentrations were input to a simple analytical model balancing advection and first-order biodegradation of hydrocarbons. First-order rate constants for the major hydrocarbon compounds were used to calibrate the model to the concentration profiles. Rate constants for the seven individual hydrocarbon compounds varied by a factor of 4. Compounds with lower molecular weights, fewer methyl groups, and no quaternary carbons tended to have higher rate constants. The first-order rate constants were consistent with kinetic parameters determined from both microcosm and tubing cluster studies at the field site.     

Micellar-Enhanced Ultrafiltration and Air Stripping for Surfactant-Contaminant Separation and Surfactant Reuse

Micellar-enhanced ultrafiltration (MELT) and air stripping were evaluated for surfactant-contaminant separation and surfactant recovery. Two linear alkyl diphenyloxide disulfonate (DPDS) surfactants were evaluated with the contaminants naphthalene and trichloroethylene. A separation model developed from micellar partitioning principles showed a good correlation to batch MEUF studies, whereas flux analysis highlighted concentration polarization effects in relation to hydrophobe length. MEUF effectively concentrated the surfactant-contaminant system (93 to 99 percent retention); however, this did not result in surfactant-contaminant separation. Batch and continuous flow air stripping models were developed based upon air/water ratio, surfactant concentration, and Micellar partitioning; model predictions were validated by experimental data. Sensitivity analyses illustrated the decline in contaminant-surfactant separation with increasing surfactant concentration (e.g., TCE removal efficiency declines from 83 percent to 37 percent as C-16 DPDS concentration increases from 0 to 55 mM). This effect is greater for more hydrophobic contaminants (naphthalene vs. TCE) and surfactants with greater solubilization potential (C16-DPDS vs. C-12 DPDS). The resulting design equations can account for this effect and thus properly size air strippers to achieve the desired removal efficiency in the presence of surfactant micelles. Proper selection and design of surfactant-contaminant separation and surfactant recovery systems are integral to optimizing surfactant-enhanced subsurface remediation.