Review: Microbial biocenoses in pristine aquifers and an assessment of investigative methods

The current knowledge of microbial biocenoses (communities) in pristine aquifers is presented in a review, which also discusses their relevance for questions of groundwater protection. Aquifers are heterogeneous on all scales and structured in a variety of habitats. The void spaces in many aquifers are small. The biocenoses are thus predominantly composed of microorganisms and, often, microinvertebrates. Larger voids and macroorganisms occur in karst cavities. Due to the absence of light, the biocenoses depend on chemical energy resources, which are, however, scarce in non-contaminated groundwater. The microorganisms thus show small cell sizes, low population densities and reduced activity; they developed specific strategies to survive oligotrophic conditions. The review also discusses the impact of contamination on the biocenoses, and the potential use of the biocenoses or specific organisms as indicators for groundwater quality, and the limits of this approach. Bacteria are either planktonic or attached to aquifer material, which requires both fluid and solid phase sampling. Most groundwater bacteria are viable but non-culturable. Consequently, cultivation techniques give an incomplete picture of the biocenoses, while methods from molecular microbiology provide genetic fingerprints of the entire community. Different analytical methods are available to count microorganisms, identify species, characterise microbial diversity, and measure activity.     

Study of geothermal water intrusion due to groundwater exploitation in the Puebla Valley aquifer system, Mexico

Significant intrusion of geothermal water into fresh groundwater takes place in the Puebla Valley aquifer system, Mexico. The decline in the potentiometric surface due to the overexploitation of the groundwater induces this intrusion. This hydrological system comprises three aquifers located in Plio-Quaternary volcanic sediments and Mesozoic calcareous rocks. The hydraulic balance of the aquifer shows that the annual output exceeds the natural inputs by 12 million m³. Between 1973 and 2002, a drop in the potentiometric surface, with an 80 m cone of depression, was identified in a 5-km-wide area located southwest of the city of Puebla. Chemical analyses performed on water samples since 1990 have shown an increase in total dissolved solids (TDS) of more than 500 mg/L, coinciding with the region showing a cone of depression in the potentiometric surface. A three-dimensional flow and transport model, based on the hydrogeological and geophysical studies, was computed by using the MODFLOW and MT3D software. This model reproduces the evolution of the aquifer system during the last 30 years and predicts for 2010 an additional drawdown in the potentiometric surface of 15 m, and an increase in the geothermal water intrusion.     

Monte Carlo simulation of mixing in Ca3Fe2Ge3O12–Ca4Ge4O12 garnets and implications for the thermodynamic stability of pyrope–majorite solid solution

Static lattice energy calculations, based on empirical pair potentials, were performed for a large set of structures differing in the arrangement of octahedral cations within the garnet 2 × 2 × 2 supercell. The compositions of these structures varied between Ca₃Fe₂Ge₃O₁₂ and Ca₄Ge₄O₁₂. The energies were cluster expanded using pair and quaternary terms. The derived ordering constants were used to constrain Monte Carlo simulations of temperature-dependent mixing properties in the ranges of 1,073–3,673 K and 0–10 GPa. The free energies of mixing were calculated using the method of thermodynamic integration. The calculations predict a wide miscibility gap between Fe-rich (cubic) and Fe-pure (tetragonal) garnets consistent with recent experimental observations of Iezzi et al. (Phys Chem Miner 32:197–207, 2005). It is shown that the miscibility gap arises due to a very strong cation ordering at the Fe-pure composition, driven by the charge difference between Ca²⁺ and Ge⁴⁺ cations. The structural and thermodynamic analogies between Ca–Ge and Mg–Si systems suggest that a similar miscibility gap should exist between pyrope and Mg–Si-majorite.     

U–Pb and Hf isotopic analysis of zircon in lower crustal xenoliths from the Navajo volcanic field: 1.4 Ga mafic magmatism and metamorphism beneath the Colorado Plateau

Zircon from lower crustal xenoliths erupted in the Navajo volcanic field was analyzed for U–Pb and Lu–Hf isotopic compositions to characterize the lower crust beneath the Colorado Plateau and to determine whether it was affected by ∼1.4 Ga granitic magmatism and metamorphism that profoundly affected the exposed middle crust of southwestern Laurentia. Igneous zircon in felsic xenoliths crystallized at 1.73 and 1.65 Ga, and igneous zircon in mafic xenoliths crystallized at 1.43 Ga. Most igneous zircon has unradiogenic initial Hf isotopic compositions (ɛ_Hf=+4.1–+7.8) and 1.7–1.6 Ga depleted mantle model ages, consistent with 1.7–1.6 Ga felsic protoliths being derived from “juvenile” Proterozoic crust and 1.4 Ga mafic protoliths having interacted with older crust. Metamorphic zircon grew in four pulses between 1.42 and 1.36 Ga, at least one of which was at granulite facies. Significant variability within and between xenoliths in metamorphic zircon initial Hf isotopic compositions (ɛ_Hf=−0.7 to +13.6) indicates growth from different aged sources with diverse time-integrated Lu/Hf ratios. These results show a strong link between 1.4 Ga mafic magmatism and granulite facies metamorphism in the lower crust and granitic magmatism and metamorphism in the exposed middle crust.     

A rationale for beach selection by the public on the coast of Wales, UK

Questionnaires were completed by 2306 beach users at 19 Welsh beaches with respect to beach selection parameters. The modal group of respondents was female aged 30–39. Beach choice was primarily determined by clean litter-free sand and seawater, followed by safety. Refreshment facilities and beach awards were deemed minor considerations by the public when choosing a beach to visit. Approximately 58 per cent of respondents were aware of beach award and rating schemes. Of coastal visitors interviewed for this paper, 67 per cent rated a beach as 'important' or 'very important' to their holiday, with just 2 per cent replying that they were unimportant.     

A chronological assessment of Lake Okeechobee (Florida) sediments using multiple dating markers

Reconstructing recent limnological history often relies on lead-210 dating to accurately ascribe a chronology to a sediment profile. In Lake Okeechobee, Florida, a large, shallow subtropical lake that may experience severe mixing, multiple dating methods are required to confirm that conformable sedimentation has been preserved and that the assumptions of the ²¹⁰Pb method are satisfied. This study uses stratigraphic profiles of heavy metals, ¹³⁷Cs, PCBs and pollen as independent dating markers to validate the sediment chronology as determined by ²¹⁰Pb for three cores from the central mud zone of the lake. Unsupported ²¹⁰Pb and most dating markers show distinct concentration/depth profiles, suggesting that the sediments have not been severely mixed for at least the last 75 years. Onset and maximum activity of the radioisotope ¹³⁷Cs in the cores coincides with the ²¹⁰Pb-dated interval of 1945–1970. This agrees well with the known timing of atmospheric deposition of ¹³⁷Cs that resulted from above-ground nuclear testing during late 1940s until 1963. Sediment core profiles of atmospherically deposited metals such as Zn and Pb, which reflect regional increases during industrialization and decreases after regulation in the 1970s, exhibit expected concentration increases and peaks coinciding within 5–15 years of the predicted ²¹⁰Pb dates. Uranium, a contaminant in some phosphate fertilizers, shows large concentration increases at core depths dated to be about 1950 by ²¹⁰Pb, matching the intensification of agriculture after WWII. PCBs, which are expected to peak in the early 1970s, were measured in one core, and the observed peak corresponds to a ²¹⁰Pb date of about 1960. Pollen makers were unable to verify specific events, but increases in disturbance taxa and declines in native types correspond generally with the expected dates assigned by ²¹⁰Pb dating. Conformity between the ²¹⁰Pb defined dates and independent markers of < ±15 years confirm that Lake Okeechobee sediments do preserve a sequential and reliable stratigraphic history of the lake, useful for reconstructing past limnological conditions.     

Mineralogical and chemical evolution of the Ernest Henry Fe oxide–Cu–Au ore system, Cloncurry district, northwest Queensland, Australia

The Ernest Henry Cu–Au deposit was formed within a zoned, post-peak metamorphic hydrothermal system that overprinted metamorphosed dacite, andesite and diorite (ca 1740–1660 Ma). The Ernest Henry hydrothermal system was formed by two cycles of sodic and potassic alteration where biotite–magnetite alteration produced in the first cycle formed ca 1514±24 Ma, whereas paragenetically later Na–Ca veining formed ca 1529 +11/−8 Ma. These new U–Pb_titanite age dates support textural evidence for incursion of hydrothermal fluids after the metamorphic peak, and overlap with earlier estimates for the timing of Cu–Au mineralization (ca 1540–1500 Ma). A distal to proximal potassic alteration zone correlates with a large (up to 1.5 km) K–Fe–Mn–Ba enriched alteration zone that overprints earlier sodic alteration. Mass balance analysis indicates that K–Fe–Mn–Ba alteration—largely produced during pre-ore biotite- and magnetite-rich alteration—is associated with K–Rb–Cl–Ba–Fe–Mn and As enrichment and Na, Ca and Sr depletion. The aforementioned chemical exchange almost precisely counterbalances the mass changes associated with regional Na–Ca alteration. This initial transition from sodic to potassic alteration may have been formed during the evolution of a single fluid that evolved via alkali exchange during progressive fluid-rock interaction. Cu–Au ore, dominated by co-precipitated magnetite, minor specular hematite, and chalcopyrite as breccia matrix, forms a pipe-like body at the core of a proximal alteration zone dominated by K-feldspar alteration. Both the core and K-feldspar alteration overprint Na–Ca alteration and biotite–magnetite (K–Fe) alteration. Ore was associated with the concentration of a diverse range of elements (e.g. Cu, Au, Fe, Mo, U, Sb, W, Sn, Bi, Ag, F, REE, K, S, As, Co, Ba and Ca). Mineralization also involved the deposition of significant barite, K(–Ba)–feldspar, calcite, fluorite and complexly zoned pyrite. The complexly zoned pyrite and variable K–(Ba)–feldspar versus barite associations are interpreted to indicate fluctuating sulphur and/or barium supply. Together with the alteration zonation geochemistry and overprinting criteria, these data are interpreted to indicate that Cu–Au mineralization occurred as a result of fluid mixing during dilation and brecciation, in the location of the most intense initial potassic alteration. A link between early alteration (Na–Ca and K–Fe) and the later K-feldspathization and the Cu–Au ore is possible. However, the ore-related enrichments in particular elements (especially Ba, Mn, As, Mo, Ag, U, Sb and Bi) are so extreme compared with earlier alteration that another fluid, possibly magmatic in origin, contributed the diverse element suite geochemically independently of the earlier stages. Structural focussing of successive stages produced the distinctive alteration zoning, providing a basis both for exploration for similar deposits, and for an understanding of ore genesis.