Use of suppressive subtractive hybridization and cDNA arrays to discover patterns of altered gene expression in the liver of dihydrotestosterone and 11-ketotestosterone exposed adult male largemouth bass (Micropterus salmoides)

In this study male largemouth bass (LMB) were exposed to the naturally occurring androgens, dihydrotestosterone (DHT) or 11-ketotestosterone (11-KT) in order to identify genes that are differentially regulated by these steroid hormones. Using subtractive hybridization on livers of fish treated with DHT against vehicle control, many novel LMB genes were cloned. These genes were added to our gene library and arrayed. Six genes were up-regulated and five were down-regulated by both androgens. But, each androgen also regulated specific genes. One gene that was identified as a potential androgen marker was spermidine-spermine-N(1)-acetyltransferase that was up-regulated by both androgens. Determining which genes are responsive to natural androgens will help to identify biochemical pathways that are impacted.     

Measurement of DNA single-strand breaks in gill and hemolymph cells of mussels, Mytilus sp., collected on the French Atlantic Coast

DNA single-strand breaks were measured by the comet assay in both gill and hemolymph cells of mussels collected in 3 sampling areas of the French coast (Pointe du Castelli, Pen Bron and Saint-Nazaire Harbour). Whole mussel tissue samples were also collected for the chemical determination of PAH, PCB and heavy metal concentrations. In mussel, a higher level of DNA strand breaks was measured in gill than in hemolymph cells (p < 0.01). Despite a factor of contamination from 2 to 3 between sites, no difference in the extent of mussel DNA strand breaks was shown between sampling locations (p > 0.05), questioning the sensitivity of the assays used in biomonitoring studies.     

北海盆地第三纪深海扇的定量分析

北海盆地第三纪深海扇的定量分析Ｔ．Ｒｅｙｎｏｌｄｓ深海扇内富存有大量的碳氢化合物。因此，无论是从扇容积作为石油储层还是在生产期提供水的补给方面来说，它们的规模、几何形态、砂的体积、纯砂体积与总体积之比以及砂的连通性都是石油工业的关键问题。但是，至今深...     

海洋微生物学的主要任务

海——微生物栖息的特殊环境。特殊的盐类成分,低温,高压,低浓度的有机物质,植物及动物区系的稀少等组成了微生物生命活动的广阔海洋区域一些主要生态特征。 海洋中最初的微生物学研究,早在上一世纪就已进行了,但足它们局限于较狭小的任务——     

Responses of a Mediterranean soft bottom community to short-term (1993-1996) hydrological changes in the Rhone river

The polychaete fauna of muddy bottoms off the Rhone delta (NW Mediterranean) was seasonally sampled at two sites at 70 m depth, from 1993 to 1996. During this period, five severe flood events occurred. A clustering analysis (distance coefficient of Whittaker) and three way fixed factor ANOVAs (site x season x year) showed strong year-to-year changes in species density and community structure, changes that masked seasonal variations. Total density increased by a factor of 3 and density of most species significantly increased following the first flooding event. Changes in the community structure were due to the successive dominance of a few species. The opportunistic species, such as Cossura sp., Mediomastus sp. and Polycirrus sp., exhibited peaks in density 1-3 months after the flood. These peaks were followed by a drastic decline. For species with a long life span, such as Sternaspis scutata, a slower but continuous increase in density was observed which was maintained for several months. The density fluctuations of these species exhibited a good correlation with river flow with time lags of 1-2 years. The successional dynamics observed are explained according to the geographical origin of the floods and the biology and feeding ecology of species.     

Seasonal variation in lysosomal destabilization in oysters,Crassostrea virginica

Lysosomal destabilization assays have been used as valuable biomarkers of pollutant exposures in a variety of bivalve and fish species. The responses of oysters, Crassostrea virginica, deployed at and native to various reference and degraded sites were evaluated for lysosomal destabilization during both summer and winter seasons. In both native and deployed oysters, lysosomal destabilization rates tended to be higher during the winter at both reference and polluted sites. There are at least two hypothetical explanations. Greater lysosomal destabilization rates may be related to physiological changes associated with mobilization of nutrient reserves during the winter and gametogenesis. However, lysosomal destabilization in deployed oysters was correlated with tissue metal concentrations. These data also support a second hypothesis that seasonal differences in physico-chemical factors (such as reduced levels of acid volatile sulfides) may increase the bioavailability of metals during the winter so that adverse effects are more pronounced.     

A NEW MODEL FOR PEBBLE BEACH TRACER DISPERSAL

A series of eight tracer experiments were carried out over one year (1983) on a low energy, macro-tidal beach in South Wales, UK. Tracer movement was monitored and 30 pebbles adjacent to coloured tracer pebbles were measured. Measurements were of the A, B, and C axes, roundness, Maximum Projection Sphericity and Oblate-Prolate indices. Analysis shows that through time, the percentage rejection of these properties increased. It is theorised that the surface tracers are 'misfits' or 'rejects' which have not found their equilibrium position in the pebble facies.     

南极沿海伯尔顿盐湖双刺镰状水蚤盐度耐性的季节变化

双刺镰状水蚤Drepanopus bispinosus为南极沿海伯尔顿盐湖(Burton lake)中的越冬种类之一,其雌性性成熟大约于每年的初冬季节,在随后8个多月的生活史中,经历了一个盐度从33(5月份)到39(11月份)的环境变化.本文报道了该水蚤雌性成体对环境盐度适应的季节差异,动物在冬季的耐低盐能力较夏季强,从冬季到夏季,动物对盐度的适应范围明显地向高盐度方向偏移,这与环境盐度对动物的驯化有关.在低盐水中,动物的呼吸率增高,且夏季大于冬季,因而进一步证实了环境盐度的变化对动物盐度适应性的影响.     

印度东海岸沃姆瑟塔拉河流盆地粘土组分和全样的痕量元素地球化学

沃姆瑟塔拉河是一条中等河流，起源于山口东部的丘陵区．流域盆地的主要地质建造由榴英硅线变质岩、紫苏花岗岩和花岗岩组成．时代属太古代．流域盆地属半干旱-干旱气候。