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A comparison of three widely used GPS triple-frequency precise point positioning models

GPS Solutions - The EUREF Permanent Network (EPN) is a network of continuously operating GNSS stations installed throughout the European continent. The EPN Central Bureau (CB) performs the...     

Comparative analysis on microbial community associated with different gastrointestinal regions of wild northern snakehead Channa argus Cantor, 1842

Microbial communities in different gastrointestinal regions(stomach, foregut, midgut, and hindgut) of the northern snakehead C hanna argus(Cantor, 1842) were compared by polymerase chain reaction and partial 16 S r DNA sequencing. A total of 194, 140, 212, and 122 OTUs were detected in the stomach, foregut, midgut, and hindgut, respectively. Significant dif ferences were found in the Sobs, ACE, Shannon, and Simpson indices among samples(P 0.05). The gastrointestinal microbial community of C. argus consisted predominantly of Proteobacteria with either H alomonas, Shewanella, Plesiomonas, or Sphingomonas. Fusobacteria, Firmicutes, and Bacteroidetes also existed in the gastrointestinal tracts. However, significant diff erences were found in the compositions of microbial community among the four regions( P 0.05). Cyanobacteria and Spirochetes were significantly higher in the midgut and hindgut( P 0.05). Fusobacteria and Firmicutes were dominant in the hindgut and foregut, respectively(P 0.05). Proteobacteria was the lowest in the hindgut( P 0.05). At genus level, C etobacterium and Plesiomonas were significantly higher in the hindgut than in the other three samples( P 0.05). Clostridium and Prevotella were the highest in the midgut(P 0.05). Halomonas, Shewanella, and S phingomonas were the highest in the foregut( P 0.05). Paracoccus and Vibrio were the highest in the stomach. Several genera were only detected in certain regions, as follows: stomach, P aracoccus and Vibrio; foregut, Halomonas, Shewanella, and Sphingomonas; midgut, Clostridium and Prevotella; and hindgut, C etobacterium and Plesiomonas( P 0.05). At the species level, A cinetobacter rhizosphaerae was only detected in the stomach. P revotella copri and Clostridium perfring were not detected in the foregut and midgut, respectively, whereas Prevotella copri and Faecalibacterium pra were not detected in the hindgut. These findings provide valuable information on the microbial community in each gastrointestinal region of C. argus. Moreover, this study indicated that microbial community was not only related to rearing environment but also to the physico-chemical characteristics of each gastrointestinal region.     

机载LiDAR地形特征信息快速提取

为更有效地获取地形特征信息,提出一种机载LiDAR地形特征信息快速提取算法。首先,通过构建二次曲面拟合模型,建立实测LiDAR地形数据与拟合曲面的几何规则;然后,采用LM算法迭代参数寻优,获得最优化结果下的地形拟合参数,计算拟合时间及拟合精度;最后,以地形拟合模型为基础,进行地形特征信息的快速提取。通过机载LiDAR实测数据验证,当最优搜索半径为2 m时,地形曲面的拟合时间仅为0.02 s,RMSE仅为5.09 cm。该算法保证了地形特征信息提取效率和精度,能够有效满足机载LiDAR科学研究和工程应用的技术需求。     

Cloning and phylogenetic analysis of a fatty acid elongase gene from Nannochloropsis oculata CS179

Nannochloropsis oculata CS179, a unicellular marine microalga, is rich in long-chain polyunsaturated fatty acids (LCPUFAs). Elongase and desaturase play a key role in the biosynthesis of PUFAs. A new elongase gene, which encodes 322 amino acids, was identified via RT-PCR and 5′ and 3′ RACE. The sequence of the elongase gene was blast-searched in the NCBI GenBank and showed a similarity to those of the cryptosporidium. But the NJ-tree revealed that the N. oculata CS179 elongase clustered with those of the microalgae Phaeodactylum tricornutum, Ostreococcus tauri and Thalassiosira pseudonana.     

海洋毒素大田软海绵酸完全抗原的制备与分析

为了建立大田软海绵酸(Okadaic acid,0A)的免疫学检测方法,利用活泼酯法将OA分子中的羧基与栽体蛋白上的氨基偶联,人工制备OA的免疫抗原(OA-IgG)和检测抗原(OA-BSA),经电泳、动物实验及ELISA法进行了鉴定,完全抗原的偶联成功,为利用杂交瘤技术制备OA的单克隆抗体和海产品OA免疫学检测方法奠定了良好的基础.     

苏鲁豫皖省际边界区经济发展空间特征与类型划分

边界区类型划分与经济发展水平密切相关,对省际边界区经济协调发展具有参考价值.该文以苏鲁豫皖省际边界区为研究对象,利用空间自相关分析、产业差异度等方法,分析该地区经济发展的时空特征,并从产业结构视角划分边界区类型和边界线类型.主要结论如下:1)苏鲁豫皖省际边界区经济集聚特征明显且不断增强,并出现地域分化现象,各省域内县域经济发展水平差异不断减小,而省域间经济差异在扩大;2)经济发展水平高值区和低值区集聚趋势明显,省际边界效应带来的经济差异呈现显著的地域特征;3)根据省际边界两侧相邻地区的产业结构差异,将边界区划分为"桥梁型"和"切变型",将省际边界线划分为"桥梁型""切变型"和"中间型".     

基于倾斜摄影技术的戈壁灌丛沙丘形态反演及其对粉尘释放研究的意义

灌丛沙丘是广泛分布于西北干旱区的风沙地貌。戈壁上发育的灌丛沙丘在戈壁沙尘释放和固沙能力方面具有重要的指示意义和应用价值。本研究采用倾斜摄影技术对戈壁上的灌丛沙丘形态特征进行观测和提取,并分析沉积物特征,计算戈壁灌丛沙丘的固沙能力,为沙尘释放研究提供依据。结果表明:(1)戈壁上发育的灌丛沙丘有明显的空间分布格局,即主要分布在干河床的两侧和地势低洼地区。(2)戈壁灌丛沙丘在形态上和其他地表灌丛沙丘类似,但沙丘的长度、宽度、底面积、体积等小得多,而且参数之间具有明显的相关关系。(3)沉积物的累积概率曲线表现为三段式,跃移组分的粒度范围(50~700 μm)明显与以往研究不同。沙丘内部和表层的粉沙和黏土含量分别为4.47%和5.24%,从而说明灌丛沙丘能够捕获戈壁上释放的粉尘物质。(4)戈壁地区单个灌丛沙丘的粉尘释放量约0.05~0.08 m³,固沙能力最大可达18.71 m³。