基于数字全息显微的海洋浮游生物三维形貌快速重建方法研究

针对海洋浮游生物实时探测中,三维形貌特征难以快速获取的问题,本文提出了一种海洋浮游生物三维形貌快速重建的方法。基于离轴菲涅尔数字全息显微系统,通过改进相位恢复方法预消除相位畸变,直接得到正确的相位信息,进而对浮游生物三维形貌重建。该方法不需要进行后期复杂的相位补偿计算,就可以对浮游生物三维形貌进行快速重建,有利于对动态、微小尺寸的浮游生物进行实时探测及分析。论文对青岛近海岸浮游生物桡足类和夜光虫进行了三维形貌重建,分辨率可达到3.5μm。实验结果为离轴菲涅尔数字全息显微系统用于海洋浮游生物原位、实时探测的可行性提供了依据。     

Long-term changes in the fish community structure from the Tsushima warm current region of the Japan/East Sea with an emphasis on the impacts of fishing and climate regime shift over the last four decades

Japanese fisheries production in the Japan/East Sea between 1958 and 2003 increased to their peak (1.76 million tons) in the late 1980s and decreased abruptly with the collapse of Japanese sardine. Catch results for 58 fisheries and various environmental time-series data sets and community indices, including mean trophic level (MTL) and Simpson’s diversity index (DI), were used to investigate the impacts of fishing and climate changes on the structure of the fish community in the Tsushima warm current (TWC) region of the Japan/East Sea. The long-term trend in fisheries production was largely dependent on the Japanese sardine that, as a single species, contributed up to 60% of the total production in the Japanese waters of the Japan/East Sea during the late 1980s. Excluding Japanese sardine, production of the small pelagic species was higher during 1960s and 1990s but lower during 1970s and 1980s. This variation pattern generally corresponds with the trend in water temperature, warmer before early 1960s and after 1990s but colder during 1970s and 1980s. The warm-water, large predatory fishes and cold water demersal species show opposite responses to the water temperature in the TWC region, indicating the significant impact of oceanic conditions on fisheries production of the Japan/East Sea. Declines in demersal fishes and invertebrates during 1970s and 1980s suggested some impact of fishing. MTL and DI show a similar variation pattern: higher during 1960s and 1990s but lower during 1970s and 1980s. In particular, the sharp decline during the 1980s resulted from the abundant sardine catches, suggesting that dominant species have a large effect on the structure of the fish community in the Japan/East Sea. Principal component analysis for 58 time-series data sets of fisheries catches suggested that the fish community varied on inter-annual to inter-decadal scales; the abrupt changes that occurred in the mid-1970s and late 1980s seemed to correspond closely with the climatic regime shifts in the North Pacific. These results strongly suggest that the structure of the fish community in the Japan/East Sea was largely affected by climatic and oceanic regime shifts rather than by fishing. There is no evidence showing “fishing down food webs” in the Japan/East Sea. However, in addition to the impacts of abrupt shifts that occurred in the late 1980s, the large predatory and demersal fishes seem to be facing stronger fishing pressure with the collapse of the Japanese sardine.     

热带西太平洋Y3和M2海山微食物网主要类群生态分布与比较

2014年12月和2016年3月分别对热带西太平洋Y3海山(中层海山)和M2海山(浅海山)微食物网主要类群(包括聚球藻、原绿球藻、微微型真核浮游生物、异养细菌和浮游纤毛虫)丰度和生物量垂直分布进行了研究。结果表明,Y3和M2海山水文环境比较相似但略有区别,叶绿素最大值层(DCM)分别在75—100m和110m水层,微食物网各主要类群在垂直尺度上的分布与叶绿素a浓度紧密相关。其中浮游纤毛虫呈现"双峰型"模式,即丰度高值出现在表层和DCM层;原绿球藻和微微型真核浮游生物呈现"单峰型"模式,丰度高值出现在DCM层;聚球藻和异养细菌峰型相对不显著,DCM层以浅丰度较高,DCM层以深丰度明显降低。分析其原因,可能是受到温度、光照和营养盐的共同影响。Y3和M2海山微食物网结构的垂直变化不完全一致。其中,Y3海山30m以浅和150m以深异养细菌生物量占绝对优势,75—100m水层自养型生物(原绿球藻和微微型真核浮游生物)占绝对优势;M2海山75m以浅和200m以深异养细菌占绝对优势,110—150m自养型生物占绝对优势。M2海山自养型生物占优势的水层要明显深于Y3海山,可能与它们的海山类型和采样季节不同有关。     

莺歌海盆地中新统轴向重力流沉积特征及勘探潜力

轴向重力流沉积是一种重要的深水储层,其形成的岩性油气藏也是目前莺歌海盆地重点勘探领域。通过钻井、测井、地震和区域地质等资料的综合研究,分析莺歌海盆地中新统轴向重力流沉积特征和演化规律,探讨轴向重力流岩性油气藏的成藏条件和控制因素。结果表明,中新统储层为重力流沉积成因的厚层细砂岩,主水道和朵叶复合体是重力流沉积有利的沉积微相;中新世海南物源供给充足、断裂坡折带发育以及盆地轴向负向地形是该区形成轴向重力流沉积的宏观地质条件,在中新世各个时期形成了一系列沿盆地轴向分布、具有前积反射结构特征的轴向重力流沉积。研究区中新统轴向重力流储层厚度大、沉积规模广、临近烃源岩、构造脊微裂隙发育、圈闭保存好,具备优越的岩性油气藏成藏条件,是盆地下一步勘探的重要领域。     

On the Conversion of Antarctic Ice-Mass Change to Sea Level Equivalent

This technical note aims to provide a quick reference and some computational examples for the conversion between Antarctic ice-mass changes and global sea level equivalent (SLE) changes using a few assumptions that computationally simplify this complex problem and that acknowledge gaps in our knowledge of the Antarctic environment. A number of factors involved in the conversion process are discussed, and the sensitivity of the conversion result to certain aspects is analyzed. It was found that the global ocean area calculated from a recently improved global shoreline dataset has little impact on the uncertainty of the SLE estimation. SLE estimation using satellite gravity observations, such as those by GRACE, are sensitive to glacial isostatic adjustment (GIA) models. One more important result from the computation is that the effective density of the volume that is gained or lost during mass change may greatly affect the outcome of the conversion if it differs greatly from the actual density of the firn/ice layers. Finally, a table of computational examples is provided for reference under some assumptions for simplifying the computation.     

A Lagrangian particle random walk model for simulating a deep-sea hydrothermal plume with both buoyant and non-buoyant features

This paper presents a computational model of simulating a deep-sea hydrothermal plume based on a Lagrangian particle random walk algorithm. This model achieves the efficient process to calculate a numerical plume developed in a fluid-advected environment with the characteristics such as significant filament intermittency and significant plume meander due to flow variation with both time and location. Especially, this model addresses both non-buoyant and buoyant features of a deep-sea hydrothermal plume in three dimensions, which significantly challenge a strategy for tracing the deep-sea hydrothermal plume and localizing its source. This paper also systematically discusses stochastic initial and boundary conditions that are critical to generate a proper numerical plume. The developed model is a powerful tool to evaluate and optimize strategies for the tracking of a deep-sea hydrothermal plume via an autonomous underwater vehicle (AUV).     

Modeling the long-term variability of phytoplankton functional groups and primary productivity in the South China Sea

Primary productivity (PP) and phytoplankton structure play an important role in regulating oceanic carbon cycle. The unique seasonal circulation and upwelling pattern of the South China Sea (SCS) provide an ideal natural laboratory to study the response of nutrients and phytoplankton dynamics to climate variation. In this study, we used a three-dimensional (3D) physical–biogeochemical coupled model to simulate nutrients, phytoplankton biomass, PP, and functional groups in the SCS from 1958 to 2009. The modeled results showed that the annual mean carbon composition of small phytoplankton, diatoms, and coccolithophores was 33.7, 52.7, and 13.6 %, respectively. Diatoms showed a higher seasonal variability than small phytoplankton and coccolithophores. Diatoms were abundant during winter in most areas of the SCS except for the offshore of southeastern Vietnam, where diatom blooms occurred in both summer and winter. Higher values of small phytoplankton and coccolithophores occurred mostly in summer. Our modeled results indicated that the seasonal variability of PP was driven by the East Asian Monsoon. The northeast winter monsoon results in more nutrients in the offshore area of the northwestern Luzon Island and the Sunda Shelf, while the southwest summer monsoon drives coastal upwelling to bring sufficient nutrients to the offshore area of southeastern Vietnam. The modeled PP was correlated with El Niño/Southern Oscillation (ENSO) at the interannual scale. The positive phase of ENSO (El Niño conditions) corresponded to lower PP and the negative phase of ENSO (La Niña conditions) corresponded to higher PP.     

棉粕蛋白酶解物对异育银鲫(Carassius auratus gibelio)消化、生长和胰蛋白酶mRNA表达量的影响

以棉粕蛋白为酶解底物,用枯草杆菌蛋白酶对其进行酶解,以酶解产物1.5%和3.0%两个梯度等量替代鱼饲料配方中棉粕,在室内流水养殖系统中喂养异育银鲫鱼种[体重为(30±2)g]65天。测定鱼的生长、营养物质表观消化率、消化蛋白酶活性及肝胰脏中胰蛋白酶mRNA表达水平等指标。结果表明,添加1.5%和3.0%棉粕酶解产物的鱼在饲养35天后的特定增长率(SGR)分别比对照组高32.5%和56.7%,且差异显著(P<0.05);在饲养65天后,两组特定增长率分别比对照组高8.0%和21.0%,且差异极显著(P<0.01),肝胰脏中胰蛋白酶mRNA表达水平也随棉粕酶解产物添加梯度提高而相应提高,表明鱼的生长与消化蛋白酶mRNA表达水平相关。同时棉粕蛋白酶解物对肠道蛋白酶的活性和营养物质表观消化率都有促进作用,而棉粕蛋白酶解物对鱼肌肉成分并没有改变,这也表明棉粕蛋白酶解物在促进鱼生长、内源酶活性同时并未降低鱼的品质。     

鳗鲡目鱼类线粒体蛋白质编码基因易位及系统演化关系分析

鳗鲡目鱼类线粒体基因组的基因组成较为保守,在58个线粒体基因组中,仅有3个物种存在基因数量的差异。在19个鳗鲡目鱼类线粒体基因组中存在主编码基因的重排。主编码基因的变异位点分析结果支持nad5、nad4和nad2基因作为cox1和lrRNA基因辅助的分子标记。鳗鲡科Anguillidae的20个种(亚种)聚在一起,强烈支持鳗鲡科为单系群(BPP=100)。鳗鲡科下属的3个类群(大洋洲类群、大西洋类群和印度洋-太平洋类群)也同时得到有力的验证(BPP均为100)。线鳗科Nemichthyidae和锯齿鳗科Serrivomeridae亲缘关系最近,二者聚类后,与鳗鲡科Anguillidae构成姊妹群(BPP=100)。在囊喉鱼亚目Saccopharyngoidei中,宽咽鱼科Eurypharyngidae与囊鳃鳗科Saccopharyngidae聚类(BPP=100),同时,单颌鳗科Monognathidae与月尾鳗科Cyematidae聚类(BPP=100),4个科聚在一支,支持囊喉鱼亚目为单系群(BPP=100)。在囊喉鱼亚目线粒体基因组中,3个物种(吞鳗Eurypharynx pelecanoides、拉文囊鳃鳗Saccopharynx lavenbergi和杰氏单颌鳗Monognathus jesperseni)存在主编码基因的重排。16种鳗鲡(细美体鳗Ariosoma shiroanago、短吻颈鳗Derichthys serpentinus、凯氏短尾康吉鳗Coloconger cadenati、鸭颈鳗Nessorhamphus ingolfianus、龟草鳗Thalassenchelys sp.、粗犁齿海鳗Cynoponticus ferox、百吉海鳗Muraenesox bagio、巨斑花蛇鳗Myrichthys maculosus、大吻沙蛇鳗Ophisurus macrorhynchos、几内亚副康吉鳗Paraconger notialis、哈氏异康吉鳗Heteroconger hassi、小头鸭嘴鳗Nettastoma parviceps、弱头鳗Leptocephalus sp.、斑点长犁齿鳗Hoplunnis punctata、尖吻小鸭嘴鳗Facciolella oxyrhyncha和星康吉鳗Conger myriaster),与鳗鲡目线粒体主编码基因的原始排列相比,共享nad6基因的易位。同时,基于线粒体基因组13个蛋白质编码基因构建的系统演化树,强烈支持这16个物种聚为一支(BPP=100)。然而,由此而带来的海鳗科Muraenesocidae、拟鯙科Chlopsidae和糯鳗科Congridae是否为单系群的问题,值得今后深入探究。     

海山区浮游生态学研究

本文综述了海山浮游生态学研究的现状。海山是大洋中常见的地形单元,在有的海山附近鱼类和底栖生物的生物量比周围大洋中要多,针对这些鱼类和底栖生物的饵料来源提出了三种假说,第一种假说认为海山区特殊的物理环境导致初级生产力较高(经典假说),第二种假说认为海山区的饵料不是本地生产的,地形和流场使得外来的浮游生物在海山区富集或通量加大(外来营养补充假说),第三种假说认为海山上部的颗粒有机物营养较高,是鱼类和底栖生物的营养来源之一(颗粒有机物补充假说)。对海山浮游生态学的研究基本围绕这几个假说进行。目前只在9座海山进行了浮游生态学研究,这些研究表明单个假说并不能解释海山附近鱼类生物量较大的现象,海山鱼类和底栖生物的营养来源可能有很多,这些假说阐述的机制并不互相矛盾,海山生物之间的营养关系也比原来想象的复杂得多。已有研究都是针对其中某个假说进行验证研究,建议我国即将进行的海山浮游生态学研究针对三种假说进行海上调查,以提供完备的数据支持。