Cloning,characterization,and expression analysis of the DEAD-box family genes,Fc-vasa and Fc-PL10a,in Chinese shrimp (Fenneropenaeus chinensis)

RNA helicases of the DEAD-box and related families are involved in various cellular processes including DNA replication,DNA repair,and RNA processing.However,the function of DEAD-box proteins in aquaculture species is poorly understood at molecular level.We obtained the full-length cDNA sequences of two genes encoding helicase-related proteins,Fc-vasa and Fc-PL10a,from the testes of Chinese shrimp,Fenneropenaeus chinensis.The two predicted amino acid sequences contain all the conserved motifs characterized ...     

Seismic response estimation method for earthquake‐damaged RC buildings

This study proposes a procedure for identifying spectral response curves for earthquake‐damaged areas in developing countries without seismic records. An earthquake‐damaged reinforced concrete building located in Padang, Indonesia was selected to illustrate the identification of the maximum seismic response during the 2009 West Sumatra earthquake. This paper summarizes the damage incurred by the building; the majority of the damage was observed in the third story in the span direction. The damage was quantitatively evaluated using the damage index R according to the Japanese guidelines for post‐earthquake damage evaluation. The damage index was also applied to the proposed spectral response identification method. The seismic performance of the building was evaluated by a nonlinear static analysis. The analytical results reproduced a drift concentration in the third story. The R‐index decreased with an increase in the story drift, which provided an estimation of the maximum response of the building during the earthquake. The estimation was verified via an earthquake response analysis of the building using ground acceleration data, which were simulated based on acceleration records of engineering bedrock that considered site amplification. The maximum response estimated by the R‐index was consistent with the maximum response obtained from the earthquake response analysis. Therefore, the proposed method enables the construction of spectral response curves by integrating the identification results for the maximum responses in a number of earthquake‐damaged buildings despite a lack of seismic records. Copyright © 2016 The Authors. Earthquake Engineering & Structural Dynamics published by John Wiley & Sons Ltd.     

Isotherm Study of Phosphorus Uptake from Aqueous Solution Using Aluminum Oxide

Aluminum oxide, which could be an alternative filter media for phosphorus uptake from aqueous solution, was selected as an adsorbent for the isotherm study of phosphorus uptake from aqueous solution. Batch method was adopted to investigate the adsorption behavior of phosphorus onto aluminum oxide. The Langmuir, Freundlich, and Redlich–Peterson isotherms were used to analyze the experimental data by both the linear and nonlinear regression methods. The adsorption experiment was conducted at various temperatures, to choose the appropriate method and obtain the creditable adsorption parameters for phosphorus uptake studies. The results indicated that the nonlinear regression method might be a better way to compare the best‐fitting isotherm and obtain the parameters for the adsorption of phosphorus onto aluminum oxide. Both the Redlich–Peterson and the Freundlich isotherms have high coefficients of determination for the adsorption of phosphorus onto aluminum oxide at various temperatures. In addition, a new relationship between the Redlich–Peterson and the Freundlich isotherm parameters was presented.     

Semi‐active tuned mass damper building systems: Design

Passive and semi‐active tuned mass damper (PTMD and SATMD) building systems are proposed to mitigate structural response due to seismic loads. The structure's upper portion self plays a role either as a tuned mass passive damper or a semi‐active resetable device is adopted as a control feature for the PTMD, creating a SATMD system. Two‐degree‐of‐freedom analytical studies are employed to design the prototype structural system, specify its element characteristics and effectiveness for seismic responses, including defining the resetable device dynamics. The optimal parameters are derived for the large mass ratio by numerical analysis. For the SATMD building system the stiffness of the resetable device design is combined with rubber bearing stiffness. From parametric studies, effective practical control schemes can be derived for the SATMD system. To verify the principal efficacy of the conceptual system, the controlled system response is compared with the response spectrum of the earthquake suites used. The control ability of the SATMD scheme is compared with that of an uncontrolled (No TMD) and an ideal PTMD building systems for multi‐level seismic intensity. Copyright © 2009 John Wiley & Sons, Ltd.     

A toxicity identification evaluation of silty marine harbor sediments to characterize persistent and non-persistent constituents

Sediment toxicity in silty marine harbor sediments is frequently dominated by ammonia or sulfide, leaving the adverse effects of persistent toxic substances unnoticed. To investigate the latter, we subjected interstitial water from three contaminated silty sediments to toxicity identification evaluation (TIE) phase I manipulations and tested for toxicity with four bioassays: the amphipod Corophium volutator (survival as an endpoint), the sea urchin Psammechinus miliaris (fertilization, embryo development) and the bacterium Vibrio fischeri (bioluminescence inhibition).The graduated pH manipulations identified the prominent toxicity of ammonia in the amphipod and sea urchin embryo tests, and also sulfide toxicity in the bacterium test. In two of the three samples tested with the amphipods, sea urchin embryos and bacteria, a small but significant reduction in interstitial water toxicity was achieved by removing persistent compounds through C(18) solid phase extraction. EDTA chelation resulted in a slight detoxification of the interstitial water for the amphipods and sea urchin embryos, but this was not related to any measured trace metals. Despite the presence of toxic levels of ammonia and sulfide in the harbor sediments, we established the adverse biological effects of persistent constituents by means of the TIE manipulations and in vivo interstitial water bioassays.     

Development and Implementation of Disaster Reduction Technology in Taiwan

In order to improve the efficiency of disaster reduction, the government of Taiwan has taken a number of initiatives in the development of disaster reduction technology since 1982, including the 1st, 2nd and 3rd 5-year plans for hazards prevention research, and the National Science and Technology Program for Hazards Mitigation (NAPHM). In this article, the contents and achievements of the 5-year plans and the NAPHM are briefly reviewed. On the one hand, the 5-year plans have produced meaningful research results; on the other hand, the NAPHM has provided a mechanism for integrating and translating the research results into technologies that can be implemented. The NAPHM was transformed in 2003 into the National Science and Technology Center for Disaster Reduction (NCDR) to further enhance the development and implementation of disaster technology. The mission and major future works of the NCDR are discussed.     

Some effects of sewage discharge upon phytoplankton in Hong Kong

The coastal waters of Hong Kong constitute a transition from estuarine conditions in the west to more oceanic conditions in the east, with a major discharge of untreated sewage located at the mid-point. Chlorophyll a was determined and net phytoplankton was sampled at 45 stations throughout this transition. Over a period of 20 months, chlorophyll a values rarely exceeded 2 μg l.^?1 in unpolluted coastal waters. Estuarine waters generally contained 2–6 μg l.^?1 and, in waters influenced by sewage discharge, values sometimes exceeded 20 μg l.^?1. There was no evidence of a reduction in taxonomic diversity in polluted areas except in summer, when the net phytoplankton was dominated by Chaetoceros spp. In the autumn and early winter, Skeletonema costatum was abundant in the central polluted areas.     

On Northern Hemisphere Wave Patterns Associated with Winter Rainfall Events in China

During extended winter(November–April),43% of the intraseasonal rainfall variability in China is explained by three spatial patterns of temporally coherent rainfall.These patterns were identified with empirical orthogonal teleconnection(EOT) analysis of observed 1982–2007 pentad rainfall anomalies and connected to midlatitude disturbances.However,examination of individual strong EOT events shows that there is substantial inter-event variability in their dynamical evolution,which implies that precursor patterns found in regressions cannot serve as useful predictors.To understand the physical nature and origins of the extratropical precursors,the EOT technique is applied to six simulations of the Met Office Unified Model at horizontal resolutions of 200–40 km,with and without air–sea coupling.All simulations reproduce the observed precursor patterns in regressions,indicating robust underlying dynamical processes.Further investigation into the dynamics associated with observed patterns shows that Rossby wave dynamics can explain the large inter-event variability.The results suggest that the apparently slowly evolving or quasi-stationary waves in regression analysis are a statistical amalgamation of more rapidly propagating waves with a variety of origins and properties.     

Aragonite undersaturation in Gwangyang Bay,South Korea: Effects of fresh water input

Dissolved inorganic carbon (DIC) and alkalinity were precisely measured for surface and bottom waters in Gwangyang Bay, Korea, during the four seasons to assess seasonal variations of aragonite saturation state (Ω_arag). Both the surface and bottom waters were undersaturated with respect to aragonite during summer but were supersaturated during the other seasons. The summertime undersaturation of the surface waters with respect to aragonite may have been a result of the dilution effect caused by higher seasonal river runoff. In the bottom waters, the average DIC concentration increased by 40.6 μmol kg^?1 from spring to summer, while the average alkalinity decreased by 74.7 μeq kg^?1. As a result, the alkalinity/DIC ratio decreased from 1.07 in spring to 1.01 in summer and pH also decreased from 7.91 to 7.48, which resulted in the summertime undersaturation of the bottom waters.