三江平原沼泽地碳循环初探

在三江平原多年考察与研究的基础上，通过典型沼泽的定位观测，估算了三江平原沼泽土壤中的碳储量，探讨了沼泽植物对大气ＣＯ２的固定，碳素自沼泽土壤向大气的转移，沼泽近地气层ＣＯ２流，以及沼泽植物－土壤－大气之间的碳素流动。     

A star-based method for precise wavelength calibration of the Chinese Space Station Telescope(CSST) slitless spectroscopic survey

The Chinese Space Station Telescope(CSST)spectroscopic survey aims to deliver high-quality low-resolution(R>200)slitless spectra for hundreds of millions of targets down to a limiting magnitude of about 21 mag,distributed within a large survey area(17500 deg2)and covering a wide wavelength range(255-1000 nm by three bands GU,GV,and GI).As slitless spectroscopy precludes the usage of wavelength calibration lamps,wavelength calibration is one of the most challenging issues in the reduction of slitless spectra,yet it plays a key role in measuring precise radial velocities of stars and redshifts of galaxies.In this work,we propose a star-based method that can monitor and correct for possible errors in the CSST wavelength calibration using normal scientific observations,taking advantage of the facts that(ⅰ)there are about ten million stars with reliable radial velocities now available thanks to spectroscopic surveys like LAMOST,(ⅱ)the large field of view of CSST enables efficient observations of such stars in a short period of time,and(ⅲ)radial velocities of such stars can be reliably measured using only a narrow segment of CSST spectra.We demonstrate that it is possible to achieve a wavelength calibration precision of a few km s^-1 for the GU band,and about 10 to 20 kms^-1 for the GV and GI bands,with only a few hundred velocity standard stars.Implementations of the method to other surveys are also discussed.     

Antibacterial activity of extracts from <Emphasis Type="Italic">Zostera marina</Emphasis> against pathogens of <Emphasis Type="Italic">Apostichopus japonicus</Emphasis> skin ulceration disease

The purpose of this study was to investigate the antibacterial activity of extracts from Zostera marina against the pathogens of Apostichopus japonicus skin ulceration disease. When 95% ethanol (v/v) solvent was used to extract Zostera marina at 50°C, aqueous extract (ZA) showed obvious bacteriostatic effects on the tested bacterial strains (inhibition halo diameters between 8.23 mm and 13.62 mm), whereas the ethyl acetate extract (ZE) was almost inactive. The minimal inhibitory concentration (MIC) of ZA against four pathogens were homogeneous at 12.8 g L⁻¹. ZA components were analyzed by thin layer chromatography (TLC) assay and six fractions were obtained. In another study, the six fractions showed inhibitory effects against the tested bacteria while their functions seemed to counteract the ZA activity.     

FPSO甲板上浪研究现状

浮式生产储油轮（FPSO）是当今海洋石油开发的主流设施。FPSO通常采用单点系泊系统,船首暴露在波浪作用下,定位于特定海域进行长达数年的长期作业,上浪的风险很大。甲板上浪及其产生的载荷已是当前国际FPSO工程和研究领域的热点之一。介绍了国内外在试验研究和理论研究两方面对FPSO甲板上浪研究的进展情况,并建议我国开展这方面的研究。     

Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.     

The influence of Stokes drift on oil spills: Sanchi oil spill case

Spilled oil floats and travels across the water’s surface under the influence of wind, currents, and wave action. Wave-induced Stokes drift is an important physical process that can affect surface water particles but that is currently absent from oil spill analyses. In this study, two methods are applied to determine the velocity of Stokes drift, the first calculates velocity from the wind-related formula based upon a one-dimensional frequency spectrum, while the second determines velocity directly from the wave model that was based on a two-dimensional spectrum. The experimental results of numerous models indicated that: (1) oil simulations that include the influence of Stokes drift are more accurate than that those do not; (2) for medium and long-term simulations longer than two days or more, Stokes drift is a significant factor that should not be ignored, and its magnitude can reach about 2% of the wind speed; (3) the velocity of Stokes drift is related to the wind but is not linear. Therefore, Stokes drift cannot simply be replaced or substituted by simply increasing the wind drift factor, which can cause errors in oil spill projections; (4) the Stokes drift velocity obtained from the two-dimensional wave spectrum makes the oil spill simulation more accurate.     

Accumulation and ecological effects of soil heavy metals in conventional and organic greenhouse vegetable production systems in Nanjing,China

The spatial variability of soil heavy metals in conventional and organic greenhouse vegetable production (CGVP and OGVP) systems can reveal the influence of different farming activities on their accumulation and plant uptake. This provides important basic data for soil utilization and pollution risk assessment. Based on horizontal and vertical spatial analysis, this paper presents the spatial variability and accumulation of soil heavy metals. The effects on plant uptake and factors influencing heavy metal accumulation are presented using the two typical greenhouse vegetable sites in Nanjing City, China as examples. Results showed that different greenhouse vegetable production systems had their own dominant heavy metal accumulation, specifically, Hg and Pb in CGVP system and Cd in OGVP system. The spatial analysis showed that horizontally, distribution of soil properties and heavy metal concentrations in the two sites showed decreases from specific regions to the periphery for organic matter (OM), Cd, Cu, Hg, Pb, and Zn in CGVP and OM, As, Cd, Cu, Hg, Pb, and Zn in OGVP. Vertically, soil properties and heavy metals mainly vary in the topsoil. The key factor for the accumulation was excess fertilizer input. Variation of soil properties and the accumulation of soil heavy metals significantly influenced heavy metal uptake by plants. However, accumulation risk varied according to different heavy metals and different plant species. Environmental management of these two kinds of production systems should pay more attention to fertilizer application, plant selection, and soil properties.