Ion balance,acid-base regulation,and chloride cell function in the common killifish,Fundulus heteroclitus—a euryhaline estuarine teleost

The common killifish,Fundulus heteroclitus, is a euryhaline teleost common throughout estuaries of eastern North America. This symposium paper reviews the important contributions of the killifish to our present understanding of ionoregulation in seawater (SW) fish and their mechanisms of euryhalinity, and presents new data developing the killifish as a freshwater (FW) model system. Experiments on killifish have characterized (i) drinking in SW and its reduction in FW; (ii) the adaptive roles of the kidney to SW and FW conditions; (iii) the instantaneous (Phase I) and delayed (Phase II) reductions in Na⁺ outflux that occur upon transfer from SW to FW; (iv) the importance of prolactin secretion in the Phase II effect; (v) the cortisol-stimulated induction of branchial Na⁺, K⁺-ATPase that occurs upon transfer from FW to SW; (vi) the accompanying changes in morphology of the mitochondria-rich (MR) or “chloride cells” on the gills; (vii) the localization of this Na⁺, K⁺-ATPase activity to the basolateral membrane of chloride cells; and (viii) the NaCl-secretory function of these cells in SW. The opercular epithelium, which is rich in chloride cells, has been used as an in vitro model to characterize the mechanisms and control of NaCl secretion in SW fish. Much less is known about gill function in fresh water (inward NaCl transport), primarily due to the absence of a comparable freshwater model. Here we show that killifish acclimated to dilute FW ([NaCl] = 1 mmol I^?1) possess large numbers of MR cells on the opercular epithelium. When mounted in vitro with FW on the outside, the preparation develops a large inside negative transepithelial potential (TEP) that is a Na⁺ diffusion potential. By the Ussing flux ratio criterion, Na⁺ fluxes are passive, but a small active influx of Cl^? occurs, an observation that supports the involvement of MR cells in active Cl^? uptake. This FW opercular epithelium if bathed with isotonic saline on both sides does not secrete Cl^?, indicating that the MR cells indeed are of the FW type. In vivo, the fish exhibits a high rate of Na⁺ influx and outflux; Cl^? outflux is much lower, and there is no detectable Cl^? influx. Experimental variation of FW [NaCl] reveals a saturable, low affinity Na⁺ uptake mechanism, a Cl^? influx mechanism that is activated only at much higher concentrations, and no evidence of exchange diffusion. Acid-base disturbance appears to be corrected by differential regulation of the outflux components only. Hence, the FW killifish ionoregulates somewhat differently from the few other FW teleosts that have been examined, and its opercular epithelium will serve as a very useful model system.     

Geographical Dimensions of Environmental Restructuring in New Zealand*

During the 1980s, New Zealand underwent a period of dramatic economic, social, and administrative restructuring. The reform extended to the administrative arrangements for environmental management. A geographic restructuring model is used in this paper to establish the context in which the reforms were carried out. A combination of economic, environmental, and social influences operating at different geographic scales can be identified. These influences are subsequently illustrated through reference to three aspects of the restructuring that have distinct geographical dimensions: the definition of human-environment relations, the spatial definition of planning regions, and the implications of spatial differentiation for resource management policy and practice.     

Recent crustal movements in The Central Sierra Nevada—Walker lane region of California—Nevada: Part iii, The Olinghouse fault zone

The Olinghouse fault zone is one of several NE—ENE-trending fault zones and lineaments, including the Midas Trench and the Carson—Carson Sink Lineament, which exhibit left-lateral transcurrent movement conjugate to the Walker Lane in western Nevada. The active portion of this fault zone extends for approximately 23 km, from 16 km east of Reno, Nevada, to the southern extent of Pyramid Lake. The fault can be traced for most of its length from its geomorphic expression in the hilly terrain, and it is hidden only where overlain by recent alluvial sediments. Numerous features characteristic of strike-slip faulting can be observed along the fault, including: scarps, vegetation lines, sidehill and shutter ridges, sag ponds, offset stream channels and stone stripes, enclosed rhombohedral and wedge-shaped depressions, and en-echelon fractures.A shear zone having a maximum observable width of 1.3 km is defined principally by Riedel shears and their symmetrical P-shears, with secondary definition by deformed conjugate Riedel shears. Several continuous horizontal shears, or principal displacement shears, occupy the axial portion of the shear zone. The existence of P-shears and principal displacement shears suggests evolution of movement along the fault zone analogous to the “Post-Peak” or “Pre-Residual Structure” stage.Historic activity (1869) has established the seismic potential of this zone. Maximum intensities and plots of the isoseismals indicate the 1869 Olinghouse earthquake had a magnitude of 6.7. Field study indicates the active length of the fault zone is at least 23 km and the maximum 1869 displacement was 3.65 m of left-slip. From maximum fault length and maximum fault displacement to earthquake magnitude relations, this corresponds to an earthquake of about magnitude 7.