Identification and characterization of 43 microsatellite markers derived from expressed sequence tags of the sea cucumber (Apostichopus japonicus)

The sea cucumber Apostichopus japonicus is a commercially and ecologically important species in China. A total of 3056 potential unigenes were generated after assembling 7597 A. japonicus expressed sequence tags (ESTs) downloaded from Gen-Bank. Two hundred and fifty microsatellite-containing ESTs (8.18%) and 299 simple sequence repeats (SSRs) were detected. The average density of SSRs was 1 per 7.403 kb of EST after redundancy elimination. Di-nucleotide repeat motifs appeared to be the most abundant type with a percentage of 69.90%. Of the 126 primer pairs designed, 90 amplified the expected products and 43 showed polymorphism in 30 individuals tested. The number of alleles per locus ranged from 2 to 26 with an average of 7.0 alleles, and the observed and expected heterozygosities varied from 0.067 to 1.000 and from 0.066 to 0.959, respectively. These new EST-derived microsatellite markers would provide sufficient polymorphism for population genetic studies and genome mapping of this sea cucumber species.     

洛阳分县温度周滚动预报系统

利用洛阳9县(市)2002年11月~2004年9月逐日最高、最低气温资料,应用欧洲中心数值预报产品,建立了洛阳9个县(市)的温度周滚动预报方程。2004~2005年试报结果表明:24~48 h预报准确率在70%左右,绝对误差在2℃以内;24~144 h预报准确率在62%~70%之间,绝对误差在2℃左右;最低气温预报效果要好于最高气温,最低气温的绝对误差与准确率分别为1.98℃和67%,最高气温的绝对误差与准确率分别为2.28℃和61%。     

Post-earthquake investigation on several geosynthetic-reinforced soil retaining walls and slopes during the Ji-Ji earthquake of Taiwan

This paper gives an overview on the application of geosynthetic-reinforced soil structures in Taiwan. Taiwan has an unique topography and geotechnical conditions that rendered a less conservative and more challenging design compared to that of North America, Europe and Japan. The Ji-Ji (Chi-Chi) earthquake of 1999 gave an opportunity to examine the behavior of reinforced soil structures. The performance of several modular-block reinforced soil retaining walls and reinforced slopes at the vicinity of the fault was evaluated. Reinforced structures performed better than unreinforced soil retaining walls. The failure cases were highlighted and the cause of failure was identified. The lack of seismic design consideration could be a major cause of failure. The compound failure mode, the inertia force of the blocks, and the connection stiffness and strength relative to the large dynamic earth pressure, were among major items that would warrant further design consideration.     

Evaluation of bending moments and shear forces at unit connections of very large floating structures using hydroelastic and rigid body analyses

This paper investigates the characteristics of bending moments, shear forces and stresses at unit connections of very large floating structures (VLFS) under wave loads. The responses of VLFS are calculated by solving multi-body motion equation considering hydroelasticity and connection stiffness. Hydroelastic responses are calculated by the direct method. Higher-order boundary element method (HOBEM) is used for fluid analysis and finite element method (FEM) is introduced for structural analysis. The equation of motion is modified to describe the unit connections by employing spring elements. Bending moments and shear forces at the connections are obtained from the dynamic equilibrium condition for pressures and inertia forces. Two types of VLFS units such as tandem arranged units and side-by-side arranged units are considered in the numerical examples. The influences of connection stiffness, wave frequency and heading angle on responses of VLFS are investigated through the numerical examples. Rigid body analysis along with hydroelastic analysis is also carried out in the numerical analysis and comparison of those two approaches is discussed.     

喀斯特流域径流量时序演变特征分析——以贵州省为例

以贵阳水文站近45年年径流量序列为例,利用Mo r let 小波变换对喀斯特流域径流量时序演变特性进行了分析预测。结果表明: ( 1)贵阳水文站年径流主要存在4a和25a 两个时间尺度的周期变化,在这两种时间尺度下,径流演变呈现出较明显的丰、枯交替振荡。( 2) 2000年以后的10a左右,年径流量有由丰转为枯、逐步下降的趋势。( 3)年平均径流量的丰、枯周期变化主要受年平均降水量的影响;人类活动对喀斯特流域年径流量有增加和减小两方面的影响,总体上会使径流量略有增加。      

Carbon isotope composition of Upper Cambrian to Lower Ordovician carbonate in Korea,and its bearing on the Cambrian–Ordovician boundary and Lower Ordovician paleoceanography

This study presents an example of locating Cambrian–Ordovician boundary in the lower Paleozoic carbonate succession in Korea using carbon isotope stratigraphy. The Yeongweol Unit of the lower Paleozoic Joseon Supergroup comprises the Upper Cambrian Wagok Formation and the Lower Ordovician Mungok Formation in the Cambrian–Ordovician transition interval. Conventionally, the boundary was placed at the lithostratigraphic boundary between the two formations. This study reveals that the boundary is positioned in the basal part of the Mungok Formation based on the carbon isotope stratigraphy coupled with biostratigraphic information of conodont and trilobite faunas. The δ¹³C curve of the Lower Ordovician Mungok Formation shows a similar trend to that of the coeval stratigraphic interval of Argentine Precordillera (Buggisch et al., 2003), suggesting that the δ¹³C curve of the Mungok Formation reflects the Early Ordovician global carbon cycle.     

基于可靠性的海洋石油平台维修策略研究

针对海洋石油平台传统维修方式依赖经验的缺陷,从可靠性分析角度来制定维修策略,包括确定最佳维修周期和制定设备的检修顺序。以胜利油田埕岛中心一号海洋石油动力平台电力系统为例,采用基于最小割集的数值仿真方法进行故障树分析,通过任务可靠度推出任务时间,实现以可靠性为中心的最佳预防性维修周期的确定。此外,对系统进行重要度分析,按关键重要度大小进行排序,从而制定设备的检修顺序。     

青藏高原东北部古喀斯特过程与环境

本文介绍了青藏高原东北部地区的古喀斯特现象，并对相关沉积进行了化学成、粘土矿物的Ｘ－射线衍射以及石英砂表面结构等气候代用指标的分析。分析结果一致，指示了温暖湿润的亚热带气候条件。重结晶方解石的裂变径迹测年结果表明，该地区古喀斯特发育于中中新世。     

西昌市泸山地区典型火后泥石流特征与成因机制研究——以响水沟左岸3#支沟为例

2020年3月30日,四川省凉山州西昌市经久乡发生森林大火,山火燃烧后形成的山火灰和烧焦土体在强降雨作用下极易启动形成泥石流,给西昌市泸山、琼海景区生态环境及当地百姓生命财产安全带来潜在地质灾害风险。以西昌市安哈镇响水沟左岸3^#支沟为例,采用野外调查、无人机航拍、地球物理探测、现场及室内实验等手段,对2020年5月1日该沟发生的火后泥石流流体特征、动力学参数及成因机制等开展研究,系统分析了山火及降雨过程对该次火后泥石流形成的影响机制。该成果可为系统认识西昌泸山“3·30”山火地区火后泥石流的运动特征、物源规模、激发雨量及其发展趋势研判提供科学参考。     

基于卫星云参数的短时降水动态估测方法

利用实时FY-2静止气象卫星红外云图和区域中尺度地面自动监测网加密降水资料,通过对云图进行参数化处理,采用动态相关方法分析长波红外分裂窗双谱组合、长波红外水汽双谱组合、亮温面积等各类卫星云参数与不同级别降水强度的关系,寻找当时与降水强度的最佳相关云参数化因子建立短时降水动态估测模型,并利用实时最新降水估测模型对未来3 ...