An alkaline unwinding assay for the detection of DNA damage in aquatic organisms

This paper reports a rapid and facile procedure for obtaining of DNA from small, whole aquatic organisms (fathead minnow, Pimephales promelas) as well as from the liver of another species (bluegill sunfish, Lepomis macrochirus) and the subsequent estimation of its double strandedness. DNA was isolated from various tissues by homogenization in a basic pH solution containing detergent followed by differential extraction using organic solvents. Additional purification was accomplished by molecular sieve chromatography. Under defined alkaline unwinding conditions, the amount of double-stranded and single-stranded DNA was determined using the bisbenzimidazole dye Hoechst 33258. The technique was used to detect the occurrence of strand breaks in DNA of fathead minnows chemically exposed to benzo[a]pyrene.     

Tetraploid induced by physical and chemical methods in Jinjiang oyster(Crassostrea rivularis)

ＴｅｔｒａｐｌｏｉｄｉｎｄｕｃｅｄｂｙｐｈｙｓｉｃａｌａｎｄｃｈｅｍｉｃａｌｍｅｔｈｏｄｓｉｎＪｉｎｊｉａｎｇｏｙｓｔｅｒ（Ｃｒａｓｓｏｓｔｒｅａｒｉｖｕｌａｒｉｓ）￥ＲｏｎｇＳｈｏｕｂａｉ，ＳｈｉＳｈｕｍｅｉ，ＭｃＱｉｒｏｎｇ，ＬｉｕＳｈａｅｑｉｏ...     

多传感器观测下带乘性噪声系统的逆向滤波与反褶积融合算法

针对多传感器观测环境下带乘性噪声系统的逆向最优滤波与反褶积融合估计问题 ,本文提出了 1种基于极大似然准则的最优融合算法。该算法中各单传感器间并行计算 ,并且融合中心与单传感器处理中心间无反向通讯 ,因而执行效率较高。仿真表明 ,该融合算法产生的逆向滤波与反褶积比单传感器处理结果有较明显提高     

Linear magnetic anomalies and tectonic development of the middle Okinawa Trough

ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅＯｋｉｎａｗａＴｒｏｕｇｈ (ＯＴ) ,ｌｏｃａｔｅｄｎｏｒｔｈｗｅｓｔｏｆｔｈｅＲｙｕｋｙｕＴｒｅｎｃｈａｎｄｔｈｅＲｙｕｋｙｕｇｕｎｔｏ ,ｅａｓｔｏｆｔｈｅＥａｓｔＣｈｉｎａＳｅａｓｈｅｌｆ,ｉｓａｂａｃｋ ａｒｃｂａｓｉｎｂｕｌｇｅｄｔｏｔｈｅＲｙｕｋｙｕＴｒｅｎｃｈ .ＴｈｅＯＴｅｘｔｅｎｄｓｆｒｏｍＫｙｕｓｈｕｉｎｔｈｅｓｏｕｔｈｗｅｓｔｏｆＪａｐａｎｔｏｔｈｅＩｌａｎＰｌａｉｎｉｎｔｈｅｎｏｒｔｈｅａｓｔｅｒｎＴａｉｗａｎ ,Ｃｈｉｎａｉｎｔｈｅｓｏｕｔｈｗｅｓｔ…     

鱼类寄生宫脂属线虫分种检索表

本文对宫脂属线虫的研究历史进行了简单的回顾,井论述了该属线虫分类中常用的一些形态学特征。依据2005年以前发表的有关宫脂属线虫的原始资料.分析了宫脂属线虫各种的形态学特征(主要包括:侧翼的有无,尾乳突的数目和分布.尾尖有无尾饰.肠盲囊和胃盲囊及食道的比值.交合刺的长度以及与体长的比值等),依据这些分类学特征编制了该属种的分类检索表。     

Internal generation of waves: Delta source function method and source term addition method

In this study, we investigate two internal wave generation methods in numerical modeling of time-dependent equations for water wave propagation, i.e., delta source function method and source term addition method, the latter of which has been called the line source method in literatures. We derive delta source functions for the Boussinesq-type equations and extended mild-slope equations. By applying the fractional step splitting method, we show that the delta source function method is equivalent to the source term addition method employing the energy velocity. This suggests that the energy velocity should be used rather than the phase velocity for the transport of incident wave energy in the source term addition method. Finally, the performance of the delta source function method is verified by accurately generating nonlinear cnoidal waves as well as linear waves for horizontally one-dimensional cases.     

Generation of random waves in time-dependent extended mild-slope equations using a source function method

We develop techniques of numerical wave generation in the time-dependent extended mild-slope equations of Suh et al. [1997. Time-dependent equations for wave propagation on rapidly varying topography. Coastal Engineering 32, 91–117] and Lee et al. [2003. Extended mild-slope equation for random waves. Coastal Engineering 48, 277–287] for random waves using a source function method. Numerical results for both regular and irregular waves in one and two horizontal dimensions show that the wave heights and the frequency spectra are properly reproduced. The waves that pass through the wave generation region do not cause any numerical disturbances, showing usefulness of the source function method in avoiding re-reflection problems at the offshore boundary.     

Effects of salinity, temperature and food type on the uptake and elimination rates of cd, cr, and zn in the asiatic clamcorbicula fluminea

Laboratory radiotracer experiments were conducted to determine assimilation efficiencies (AE) from ingested algal food and oxic sediment particles, uptake rates from the dissolved phase, and the efflux rates of Cd, Cr and Zn in the Asiatic clamCorbicula fluminea. Among three elements, AE from both algal and sediment food was greatest for Cd, followed by Zn and Cr. The AEs of tested elements from algal food(Phaeodactylum tricornutum) were consistently higher than those from sediments at a given salinity and temperature. The influence of salinity (0, 4 and 8 psu) and temperature (5, 13 and 21¼) on the metal AEs was not evident for most tested elements, except Cd AEs from sediment. The rate constant of metal uptake from the dissolved phase (k _u was greatest for Cd, followed by Zn and Cr in freshwater media. However, in saline water, thek _u of Zn were greater than those of Cd. The influx rate of all tested metals increased with temperature. The efflux rate constant was greatest for Cr (0.02 d^-1), followed by Zn (0.010~0.017 d^-1) and Cd (0.006 d^-1). The efflux rate constant for Zn in clam tissues depurated in 0 psu (0.017 d^-1) was faster than that in 8 psu (0.010 d^-1). Overall results showed that the variation of salinity and temperature in estuarine systems can considerably influence the metal bioaccumulation potential in the estuarine clamC. fluminea. The relatively high Cd accumulation capacity ofC. fluminea characterized by the high AE, high dissolved influx rate and low efflux rate, suggested that this clam species can be used as an efficient biomonitor for the Cd contamination in freshwater and estuarine environments.     

Cloning of cytochrome P450 1A (CYP1A) genes from the hermaphrodite fish Rivulus marmoratus and the Japanese medaka Oryzias latipes

To use two small fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae) and the Japanese medaka Oryzias latipes (Belloniformes) as testing models in molecular ecotoxicology, we have cloned the cytochrome P450 1A (CYP1A) gene after screening of both genomic DNA libraries, and sequenced 11,863 and 7,243 bp including all the exons and introns with promoter regions, respectively. The Rivulus and the medaka CYP1A gene consisted of seven exons (including non-coding exons) with high homology to mammals. In the promoter region, Rivulus CYP1A gene has seven xenobiotic response elements (XREs) and two metal response elements (MREs), while the Japanese medaka CYP1A gene has six XREs and four MREs. Interestingly, medaka CYP1A gene has a number of MREs at the promoter, which may affect its response on metal exposure. We describe here the gene structure of both fish CYP1A genes.     

The optimum control of thruster system for dynamically positioned vessels

An offshore vessel with a dynamic positioning system (DP system) needs fast response to produce thrust to counteract the environmental forces acting on it for the purpose of maintaining its position and heading as close as possible to the working position. Therefore, quick and effective modulation of the thrust is the problem to determine the thrust and the rotation angle of the thruster devices of the ship. This paper presents an effective optimum control for a thruster system, using the sequential quadratic method to achieve economical and effective modulation of the thrust and the direction of the thruster. An optimum control study of a 2280 tons DP coring vessel with five rotary azimuth thruster marine positioning is studied in detail, which can quickly and exactly estimate the thrusts and angles of direction of all the thrusters. The results can provide a valuable thruster system for a dynamically positioned vessel.