Analysis of medaka cytochrome P450 3A homotropic and heterotropic cooperativity

We have previously demonstrated that medaka CYP3A is associated with metabolism of several endobiotics including steroids and bile acids. In this study, we demonstrate that medaka CYP3A catalysis exhibits unusual kinetic behaviors consistent with allosteric interaction which cannot be described by hyperbolic kinetic models. Using 7-benzyloxy-4-(trifluoromethyl)-coumarin (BFC) and nonylphenol as CYP3A substrates, we describe both homotropic and heterotropic cooperative interactions. Given the role of CYP3A in maintaining the homeostatic balance for numerous endobiotics, enzymatic activation/inhibition by endocrine disruptors (EDCs) represents a putative (non-genomic) mechanism for endocrine disruption.     

Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of ≈49 kDa size, and weak signals for proteins of ≈41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of ≈56 and 59 kDa size, and also a weak immunoreaction with a protein of ≈43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14–40 ng/g DW in controls to 373–638 ng/g DW in exposed mussels.     

Simulating a heavy metal spill under estuarine conditions: Effects on the clam Scrobicularia plana

We describe the effect of heavy metals Zn, Cd, Pb and Cu on the induction of methallothioneins on the clam Scrobicularia plana along a salinity gradient simulated under laboratory conditions. The clams were exposed to constant heavy metal concentrations in a dynamic estuary simulator during a 15-day assay to investigate possible induction of metal-binding proteins in them. The concentration of heavy metals in water was analysed. Clams were analysed for methallothionein concentrations. The speciation of Zn, Cd, Pb and Cu along the salinity gradient was modelled. Zn showed the highest concentrations and its prevalent species was the free ion. Intersite differences have been observed in methallothionein concentration and related to the salinity gradient. It seems that synthesis of methallothioneins is the result of physiological forces acting in concert with the changes in the chemical speciation of metals, owing to the trace metals uptake is controlled by means of an interaction of physiology and physicochemistry.     

Response of the barrier island coastal region of southwestern Nigeria to climate and non-climate forcing

Despite threats emanating from the influence of climate and non-climate forcing on the barrier island coastal region of southwestern Nigeria, the extent of the coastal erosion is poorly understood. We report evidence of coastal erosion and sediment accumulation in the region over a 34-year period (1973–2017), using Landsat imagery at intervals of approximately six years. Landsat image corrections and various water-extraction algorithms were used to systematically delineate coastal erosion and accumulation in the area. The region was subdivided into western and eastern subregions separated by Lagos Harbour. In the west, erosion took place during the periods 1973–1979, 1979–1984, 1990–1999 and 2005–2011, whereas in the east, erosion occurred during 1973–1979, 1990–1999 and 1999–2005. Coastal sediment accumulation occurred in the east during 1979–1984, 1984–1990, 2005–2011 and 2011–2017, whereas gains in the west occurred during 1984–1990, 1999–2005 and 2011–2017. The study revealed substantial net erosion of 1 228.1 ha in the region as a whole, over the full period. Sediment accumulation accompanying the coastal erosion appears to be linked to longshore drift. Erosion between 1973 and 2011 was probably attributable to climate change (storms and tidal conditions), longshore drift, the inflow and outflow of water at Lagos Harbour, coastal morphology and, possibly, human impacts. However, the coastal changes between 2011 and 2017 were more obviously associated with human activities, such as development of the Eko Atlantic construction project. Coastal surveillance, together with the use of environmentally sensitive protective measures, could possibly help to reduce coastal erosion in the region. Careful coastal management practices, including artificial nourishing and the installation of resilient structures (e.g. seawalls), should be undertaken to protect human settlements that are already at risk from sea-level rise.     

Pacific decadal oscillation and European climatic anomalies

By using the NCEP/NCAR reanalysis data sets for 1951–2001, we study the characteristics of Pacific cyclones. It is shown that the northeast-southwest direction is predominant in the displacements of cyclones in the North Pacific. We study the variability of the field of surface atmospheric pressure in different phases of the Pacific decadal oscillation characterizing the temperature anomalies on the surface of the ocean in the region bounded by 20 and 60°N. It is shown that the decadal variations of the North Atlantic Oscillation supported by the large-scale anomalies of the Pacific decadal oscillation is the most important cause of natural decadal oscillations in the European region. We study and evaluate the regional response to the Pacific decadal oscillation by using, as an example, the analysis of variations of the discharge of European rivers. __________ Translated from Morskoi Gidrofizicheskii Zhurnal, No. 4, pp. 13–23, July–August, 2007.     

The Ectoparasitic Gastropod Boonea (= Odostomia) impressa: Population Ecology and the Influence of Parasitism on Oyster Growth Rates

Abstract. Boonea (= Odostomia) impressa is a common ectoparasite of oysters. In the laboratory, small oysters (Crassostrea virginica) parasitized by natural densities of B. impressa produced 75 % less new shell than unparasitized oysters. Shell deposition rates of previously parasitized oysters increased significantly after all B. impressa were removed. Thus, the decrease in growth rate, although significant, apparently was not permanent. B. impressa preferentially parasitized small, living oysters (≤2.5cm) in the field, even though a higher percentage of large, living oysters (>2.5cm) was available. The snails maintained an aggregated distribution on the oyster reef. The number of B. impressa per oyster clump was positively correlated with the number of living oysters per clump, however some clumps with few or no living oysters had many B. impressa. Thus, food availability only partially explained the pattern of distribution. B. impressa was very mobile. About 50 % of the population moved in one week. Reproduction occurred throughout the year with a peak period in May. Recruitment was greatest in July, however new recruits were observed throughout the year. The reduction in growth rate of parasitized oysters, the snaiľs propensity towards parasitizing small oysters and the snail's tendency to be contagiously distributed suggests that B. impressa potentially exerts a significant influence on the population structure and health of oyster populations.     

Concentrations of suspended matter and particulate cadmium,copper, lead and zinc in the Indian sector of the antarctic ocean

The concentrations of suspended matter and particulate Cd, Cu, Pb and Zn were determined for 36 samples collected at 6 stations in the Antarctic Ocean during December, 1970 and January, 1971 using membrane filters. The concentration of suspended matter was determined gravimetrically and trace metal levels were determined using anodic stripping voltammetry. For waters deeper than 100 m the concentration of suspended matter was < 100 μg l^?1. Concentrations up to 542 μg l^?1 were recorded between surface and 100 m. Individual concentrations of the metals were scattered with depth. Average concentrations of particulate metals were: Cd, 3.5 ng l^?1; Cu, 100 ng l^?1; Pb, 35 ng l^?1; and Zn, 230 ng ;l^?1 These measurements represent non-steady state conditions of early Antarctic summer as the ice pack disintegrates and biological activity increases.     

Genetic Diversity in Marine Molluscs: A Test of the Niche-Width Variation Hypothesis

Abstract. A test of the niche width variation hypothesis was conducted in the three Mediterranean littoral species of the family Trochidae: Monodonta turbinata, M. turbiformis and Gibbula richardi. Allozymic variation encoded by 26 gene loci was analyzed, and the levels of polymorphism (P), heterozygosity (H) and allele diversity per locus (A) were estimated and appear to support the niche-width variation hypothesis. The broad-niche species, M. turbiformis and G. richardi are more polymorphic and heterozygous than the relatively narrow-niche M. turbinata (P = 0.571; 0.731 and 0.417; H = 0.108; 0.116 and 0.078; A = 2.025; 2.489 and 1.965, respectively).     

Impact of sediment organic matter quality on the fate and effects of fluoranthene in the infaunal brittle star Amphiura filiformis

Hydrophobic contaminants, such as polycyclic aromatic hydrocarbons (PAHs) readily adsorb to organic matter. The aim of this study was to determine the importance of the quality of sedimentary organic matter for the uptake, biotransformation and toxicity of the PAH, fluoranthene (Flu), in the infaunal brittle star Amphiura filiformis. Brittle stars were exposed to a base sediment covered by a 2 cm Flu-spiked top layer (30 microg Flu/g dry wt. sed.), enriched to the same total organic carbon content with either refractory or labile organic matter. The labile carbon source was concentrated green flagellate: Tetraselmis spp. The refractory carbon source was lignin from a paper mill. Tissue concentrations of Flu both in disk and arm-fractions were determined as total Flu, parent Flu (i.e. untransformed), aqueous Flu-metabolites, polar Flu-metabolites and tissue residue Flu (i.e. unextractable). Our results showed that sediment particle ingestion is a pathway by which Flu can enter benthic food webs. Flu toxicity (measured as arm-regeneration), but not net accumulation, was dependent on the nutritional quality of the ingested sediment particles. Flu bioaccumulation could not be attributed solely to equilibrium partitioning between organism lipid content and organic content of the sediment. Biotransformation of Flu by brittle stars was very limited and unaffected by organic matter quality. A. filiformis contributed to the downward transport of Flu from the surface sediment to the burrow lining. The limited breakdown of parent Flu by brittle stars and/or microorganisms was relatively higher in burrows compared to surface sediment, and highest in the presence of labile organic matter. Tissue concentrations were higher in disk than in arms, but the proportion of metabolic products relative to parent Flu was higher in arms than in the disk fraction. We estimate that the yearly mobilization of sediment-associated Flu by arm-regeneration in A. filiformis is in the range of 3.8-29.4 microg total Flu eq. m(-2) year(-1) at a sediment concentration of 30 microg Flu/g dry wt. sed.