Purification, characterization and ELISA of metallothionein from red scorponifish, Sebastiscus marmoratus

INTRODUCTIONATislowKWCys-rich(aboutonethird)metal-bindingprotein,lackofaromaticaminoacidresidues.AThasbeenfoundinkidneysandliversofmanymammals,andalsoininvertebrates,microorganismsandplants.ThepresenceofanAT-likeproteinhasbeenfoundinanumberofaquaticorganisms,includingtheplaice(Overnelletal.,1977),rock-fish(Olafsonetal.,1974),goldfish(Marafant,1976),andcrabandshrimp(Olafsonetal.,1979).AThasbecomethesubjectofgreatInterestinrecentyearsduetotheirwideexistenceandmetalaffinity.Scientistsha…     

Comparison of short-term toxicity of 14 common phycotoxins (alone and in combination) to the survival of brine shrimp Artemia salina

Toxic harmful algal blooms(HABs) can cause deleterious effects in marine organisms, threatening the stability of marine ecosystems. It is well known that different strains, natural populations and growth conditions of the same toxic algal species may lead to different amount of phycotoxin production and the ensuing toxicity. To fully assess the ecological risk of toxic HABs, it is of great importance to investigate the toxic effects of phycotoxins in marine organisms. In this study, the short-te...     

Three-dimensional mesoscale eddy identification and tracking algorithm based on pressure anomalies

Journal of Oceanology and Limnology - The Kuroshio Extension (KE) is one of the most eddy-energetic regions in the global ocean. However, most mesoscale eddy studies in the region are focused on...     

Purification and characterization of novel antioxidant peptides of different molecular weights from mackerel Pneumatophorus japonicus protein hydrolysate

Mackerel(Pneumatophorus japonicus) proteins were hydrolyzed by five proteases:trypsin,papain,neutrase,acid protease,and flavourzyme.The hydrolysate treated by neutrase exhibited the highest antioxidant activity.Response surface methodology(RSM) was employed to optimize the hydrolysis conditions in an effort to obtain a mackerel protein hydrolysate(MPH) with the highest DPPH radical scavenging activity.The MPH was fractioned using a series of ultrafiltration membranes and five fractions,namely,MPH-Ⅰ(10kDa),MPH-Ⅱ(10-2.5 kDa),MPH-Ⅲ(1-2.5 kDa),MPH-Ⅳ(0.4-1 kDa),and MPH-V(below 0.4 kDa),were obtained.DPPH radical scavenging activity,reducing power,hydroxyl radical scavenging activity,and the lipid peroxidation inhibition capability of these fractions were evaluated.The fractions in molecular weights 2.5 kDa(MPH-Ⅲ,MPH-Ⅳ,and MPH-Ⅴ),which occupied 93.4%of the total fractions,showed the strongest antioxidant activity;and the antioxidant activities of the three fractions are similar to each other.Using SP Sephadex C-25 and Sephadex G-25 columns,eight fractions were obtained from the MPH(2.5 kDa).The isolated peptide I(1 664 kDa) displayed the highest DPPH radical scavenging activity.Therefore,MPH is a potential source of antioxidant peptides.     

福建强震台网数据记录系统简介

本文概要介绍了强震仪主要技术参数、强震台网计算机系统及强震记录情况。     

用三通道合成彩色图像进行云的分类解释判读

为更好地利用FY-3A气象卫星上中分辨率光谱成像仪 (MERSI) 资料高空间分辨率及多光谱的优势,细致分析云系在宏、微观方面的多重特征,首先利用平面平行辐射传输模式 (SBDART) 证明了MERSI的0.65,1.6 μm和11.25 μm通道能够分别反映云光学厚度、云粒子大小、云顶高度的信息,然后采用三通道合成彩色图像的方法,对FY-3A气象卫星云图进行云的分类解释判读。该技术可直观区分有云区、无云区,显示海陆分界,并且使不同云类在云图上体现为不同颜色。同时,FY-3A气象卫星高达1000 m甚至250 m的空间分辨率也使云的细致结构更为清晰,两项优势的叠加,大大提升了云的分类解释判读的准确程度和精细化水平。文中还尝试寻找典型云系的三通道特征值,以期为模糊C均值聚类 (FCM) 方法中聚类中心的选定提供经验值参考。     

Analysis and comparison of the tidal gravity observations obtained with LCR-ET20 spring gravimeter

Introduction The tidal variation signals of the solid Earth recorded with the modern measuring techniques can be used to provide effectively the means to cognize the Earths motion, deformation and structure character, especially to monitor the material motion in the Earths interior. In the recent 20 years, the distribution character of the global gravity field has made great progress since the continuous and deep theoretical studies in geophysics and geodesy domains. The successful manufactur…     

甘肃巴谢剖面黄土-古土壤的光释光测年研究

光释光（OSL）测年是测定第四纪沉积物年龄的一种新技术。它通过单色光束激发使晶体中储存的电离辐射能释放出来。与热释光（TL）测年相比，OSL测年可以不考虑残留的OSL信号，而且测量具有简便、迅速且准确等优点。本文用细颗粒光释光技术对甘肃巴谢剖面的黄土-古土壤进行了测定，其测年结果与14C测年结果相吻合。这表明，光释光是一项极潜力的年代学技术。     

Characteristics and variations of the East Asian monsoon system and its impacts on climate disasters in China

Recent advances in studies of the structural characteristics and temporal-spatial variations of the East Asian monsoon （EAM） system and the impact of this system on severe climate disasters in China are reviewed. Previous studies have improved our understanding of the basic characteristics of horizontal and vertical structures and the annual cycle of the EAM system and the water vapor transports in the EAM region. Many studies have shown that the EAM system is a relatively independent subsystem of the Asian- Australian monsoon system, and that there exists an obvious quasi-biennial oscillation with a meridional tripole pattern distribution in the interannual variations of the EAM system. Further analyses of the basic physical processes, both internal and external, that influence the variability of the EAM system indicate that the EAM system may be viewed as an atmosphere-ocean-land coupled system, referred to the EAM climate system in this paper. Further, the paper discusses how the interaction and relationships among various components of this system can be described through the East Asia Pacific （EAP） teleconnection pattern and the teleconnection pattern of meridional upper-tropospheric wind anomalies along the westerly jet over East Asia. Such reasoning suggests that the occurrence of severe floods in the Yangtze and Hualhe River valleys and prolonged droughts in North China are linked, respectively~ to the background interannual and interdecadal variability of the EAM climate system. Besides, outstanding scientific issues related to the EAM system and its impact on climate disasters in China are also discussed.     

Numerical estimation of REV and permeability tensor for fractured rock masses by composite element method

The Monte Carlo method is used to generate parent stochastic discrete fracture network, from which a series of fractured rock samples of different sizes and orientations are extracted. The fracture network combined with a regular grid forms composite element mesh of the fractured rock sample, in which each composite element is composed of sub‐elements incised by fracture segments. The composite element method (CEM) for the seepage is implemented to obtain the nodal hydraulic potential as well as the seepage flow rates through the fractured rock samples. The application of CEM enables a large quantity of stochastic tests for the fractured rock samples because the pre‐process is facilitated greatly. By changing the sizes and orientations of the samples, the analysis of the seepage characteristics is realized to evaluate the variation of the permeability components, the existence of the permeability tensor and the representative element volume. The feasibility and effectiveness are illustrated in a numerical example. Copyright © 2008 John Wiley & Sons, Ltd.